Cannabinoid receptor intracellular signalling: The long journey from binding sites to biological effects

Blume, Lawrence C.; Eldeeb, Khalil; Howlett, Allyn C.

doi:10.1002/9781118451281.ch2

Cited by 1 publication

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“…In addition to direct stimulation of the receptor by agonists, signaling by CB 1 R can be further modified by accessory proteins, such as β-arrestin, G protein Associated Sorting Proteins (GASP), and CRIP1a [11,12,15]. CRIP1a was initially characterized for its ability to bind to a segment of the distal C-terminal of the CB 1 R [1].…”

Section: Discussionmentioning

confidence: 99%

“…To explore the emerging roles of CRIP1a in regulating CB 1 R, our laboratory developed CRIP1a gain and loss of function transgenic neuronal clones, and reported preliminary observations of alterations in agonist-promoted CB 1 R activation and internalization by CRIP1a [14,15]. In the present study, we determined the effect of CRIP1a on CB 1 R signaling and the associated downstream consequences on cellular function.…”

Section: Introductionmentioning

confidence: 97%

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Cannabinoid receptor interacting protein (CRIP1a) attenuates CB1R signaling in neuronal cells

Blume

Eldeeb

Bass

et al. 2015

Cellular Signalling

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CB1 cannabinoid receptors (CB1R) are one of the most abundantly expressed G protein coupled receptors (GPCR) in the CNS and regulate diverse neuronal functions. The identification of GPCR interacting proteins has provided additional insight into the fine-tuning and regulation of numerous GPCRs. The Cannabinoid Receptor Interacting Protein 1a (CRIP1a) binds to the distal carboxy terminus of CB1R, and has been shown to alter CB1R-mediated neuronal function [1]. The mechanisms by which CRIP1a regulates CB1R activity have not yet been identified; therefore the focus of this investigation is to examine the cellular effects of CRIP1a on CB1R signaling using neuronal N18TG2 cells stably transfected with CRIP1a over-expressing and CRIP1a knockdown constructs. Modulation of endogenous CRIP1a expression did not alter total levels of CB1R, ERK, or forskolin-activated adenylyl cyclase activity. When compared to WT cells, CRIP1a over-expression reduced basal phosphoERK levels, whereas depletion of CRIP1a augmented basal phosphoERK levels. Stimulation of phosphoERK by the CB1R agonists WIN55212-2, CP55940 or methanandamide was unaltered in CRIP1a over-expressing clones compared with WT. However, CRIP1a knockdown clones exhibited enhanced ERK phosphorylation efficacy in response to CP55940. In addition, CRIP1a knockdown clones displayed a leftward shift in CP55940-mediated inhibition of forskolin-stimulated cAMP accumulation. CB1R-mediated Gi3 and Go activation by CP99540 was attenuated by CRIP1a over-expression, but robustly enhanced in cells depleted of CRIP1a. Conversely, CP55940-mediated Gi1 and Gi2 activation was significant enhanced in cells over-expressing CRIP1a, but not in cells deficient of CRIP1a. These studies suggest a mechanism by which endogenous levels of CRIP1a modulate CB1R-mediated signal transduction by facilitating a Gi/o-protein subtype preference for Gi1 and Gi2, accompanied by an overall suppression of G-protein-mediated signaling in neuronal cells.

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