2008
DOI: 10.1038/bjp.2008.211
|View full text |Cite
|
Sign up to set email alerts
|

Cannabinoids reduce granuloma‐associated angiogenesis in rats by controlling transcription and expression of mast cell protease‐5

Abstract: Background and purpose: Chronic inflammatory conditions, such as granulomas, are associated with angiogenesis. Mast cells represent the main cell type orchestrating angiogenesis, through the release of their granule content. Therefore, compounds able to modulate mast cell behaviour may be considered as a new pharmacological approach to treat angiogenesis-dependent events. Here, we tested the effect of selective cannabinoid (CB) receptor agonists in a model of angiogenesis-dependent granuloma formation induced … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

0
26
0

Year Published

2009
2009
2024
2024

Publication Types

Select...
8
1

Relationship

0
9

Authors

Journals

citations
Cited by 33 publications
(26 citation statements)
references
References 39 publications
0
26
0
Order By: Relevance
“…Pascale’ of Naples. Homogenization of tissues, was performed as previously reported44. Total RNA was extracted once from the tissue samples by using an ultrapure TRIzol reagent (GibcoBRL, Carlsbad, CA, USA) and used in three independent experiments.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Pascale’ of Naples. Homogenization of tissues, was performed as previously reported44. Total RNA was extracted once from the tissue samples by using an ultrapure TRIzol reagent (GibcoBRL, Carlsbad, CA, USA) and used in three independent experiments.…”
Section: Methodsmentioning
confidence: 99%
“…RNA was first retrotranscribed as previously reported44 and then realtime PCR was carried out using TaqManH SYBR Green Master Mix (Applied Biosystems). The primers for human CBS (PPH13484B-200) and β actin (PPH00073G-200 ACTB) were from QIAGEN.…”
Section: Methodsmentioning
confidence: 99%
“…41 The membranes were challenged with anti-rpL3 and anti-rpL7a (Primm), anti-p21 (BD Pharmingen), antirpS19, anti-p70S6k, anti-anti-vinculin, anti-b-actin and anti-HA-Tag (Santa Cruz Biotechnology), anti pERK 1/2 and anti-ERK 1/2 (Cell Signaling Technologies). Anti-MDM2 antibodies were kindly provided by Fabiola Moretti (CNR-Santa Lucia).…”
Section: Western Blottingmentioning
confidence: 99%
“…Specifically, PEA reduces mast cell infiltration [14], inhibits mast cell degranulation [15], and suppresses the expression and release of mast cell mediators, including histamine, prostaglandin D(2), tumor necrosis factor(TNF)-α, chymase, tryptase, nerve growth factor, and serotonin [14,16]. Correlative data suggests that PEA is effective in several mast-cell mediated experimental models of inflammation [8], and PEA has been suggested as a novel pharmacological approach to treat mast cell regulated disorders [8,17]. …”
Section: Introductionmentioning
confidence: 99%