Cannabis and Pain Control After Total Hip and Knee Arthroplasty

Kayani, Babar; Howard, L.C.; Neufeld, Michael E.; Garbuz, Donald S.; Masri, Bassam A.

doi:10.1016/j.ocl.2023.04.002

Cited by 2 publications

(1 citation statement)

References 46 publications

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“…However, the pathogenesis of KOA is unclear, and because this disease is closely related to age, the prevalence of KOA is increasing annually as the population ages, and it has become the most prevalent musculoskeletal disease worldwide [2]. Currently, joint replacement is often used to improve symptoms in the treatment of end-stage KOA, but the high price of joint replacement surgery and the long duration of rehabilitation and analgesia after surgery should not be ignored [3,4,5]. The timely use of effective and conservative treatments relieves not only the clinical symptoms of KOA but also the side effects of arthroplasty; therefore, the development of new and more effective treatment modalities is a priority in the study of KOA[6,7].…”

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confidence: 99%

Adipose mesenchymal stem cells derived exosomes ameliorates KOA cartilage damage and inflammation by activation of PINK1-mediated mitochondrial autophagy

Kang,

Jie,

et al. 2024

Preprint

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Knee osteoarthritis (KOA) is characterized by degenerative destruction of knee cartilage. Adipose tissue-derived mesenchymal stem cells (MSCs) have been widely used in the clinic to treat joint diseases, and the exosomes secreted by adipose tissue-derived MSCs (ADSC-Exos) are more stable and easier to store than stem cell therapy alone. The aim of this study was to investigate whether ADSC-Exos could reduce KOA chondrocyte damage and inflammation by activating mitochondrial autophagy. In vitro, we induced a KOA chondrocyte model with lipopolysaccharide (LPS), and after treatment with ADSC-Exos, we assessed chondrocyte damage and inflammation by using HE, Senna O solid green, and Alcian blue staining and IL-1β immunofluorescence analysis. We also labeled chondrocytes and assessed their intracellular levels of reactive oxygen species (ROS) using the DCFH-DA probe, assessed the mitochondrial membrane potential of chondrocytes using a mitochondrial membrane potential detection kit (JC-1). In vivo, we constructed a KOA rat model by anterior cruciate ligament tenotomy (ACLT) surgery, treated the knee joint with a local injection of ADSC-Exos, reconstructed the knee joint in three dimensions using micro-CT, and evaluated the pathological changes in cartilage tissues by using HE, Senna O solid green and alcian blue staining. The in vivo and in vitro results showed that ADSC-Exos upregulated the expression of PINK1/Parkin pathway components, promoted mitochondrial autophagy in chondrocytes, increased the mitochondrial membrane potential, protected mitochondrial function in chondrocytes, and ameliorated the degradation of the cartilage matrix and inflammation during KOA.

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confidence: 99%