Cannabis smoke condensate induces human gingival epithelial cell damage through apoptosis, autophagy, and oxidative stress

Tazi, Neftaha; Semlali, Abdelhabib; Loubaki, Lionel; Amri, Abdullah Al; Rouabhia, Mahmoud

doi:10.1016/j.archoralbio.2022.105498

Cited by 7 publications

(5 citation statements)

References 49 publications

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“…RT2 Profiler PCR arrays (PAHS-020ZD-6 and PAHS-012ZD-6, Qiagen) were used to screen for a comprehensive panel of relevant genes involved in cell cycle and apoptosis, as previously described by Loubaki et al 29 and Tazi et al 35 . Briefly, 3 × 10 5 OC cells were stimulated with 10 µM carnosol (IC 50 representative value) for 24 h. PCR was performed using PCR mixture components, in accordance with the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

Cellular mechanisms mediating the anti-cancer effects of carnosol on gingiva carcinoma

Gassib,

Issa,

Loubaki

et al. 2024

Sci Rep

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Carnosol, a rosemary polyphenol, displays anticancer properties and is suggested as a safer alternative to conventional surgery, radiotherapy, and chemotherapy. Given that its effects on gingiva carcinoma have not yet been investigated, the aim of this study was to explore its anti-tumor selectivity and to unravel its underlying mechanisms of action. Hence, oral tongue and gingiva carcinoma cell lines exposed to carnosol were analyzed to estimate cytotoxicity, cell viability, cell proliferation, and colony formation potential as compared with those of normal cells. Key cell cycle and apoptotic markers were also measured. Finally, cell migration, oxidative stress, and crucial cell signaling pathways were assessed. Selective anti-gingiva carcinoma activity was disclosed. Overall, carnosol mediated colony formation and proliferation suppression in addition to cytotoxicity induction. Cell cycle arrest was highlighted by the disruption of the c-myc oncogene/p53 tumor suppressor balance. Carnosol also increased apoptosis, oxidative stress, and antioxidant activity. On a larger scale, the alteration of cell cycle and apoptotic profiles was also demonstrated by QPCR array. This was most likely achieved by controlling the STAT5, ERK1/2, p38, and NF-ĸB signaling pathways. Lastly, carnosol reduced inflammation and invasion ability by modulating IL-6 and MMP9/TIMP-1 axes. This study establishes a robust foundation, urging extensive inquiry both in vivo and in clinical settings, to substantiate the efficacy of carnosol in managing gingiva carcinoma.

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Section: Methodsmentioning

confidence: 99%

Cellular mechanisms mediating the anti-cancer effects of carnosol on gingiva carcinoma

Gassib,

Issa,

Loubaki

et al. 2024

Sci Rep

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“…The MTS assay evaluates the metabolic activity and viability of cells by converting the yellow color MTS to a purple formazan in the presence of phenazine methosulfate. The absorbance reading at 490 nm of the resulting formazan solution is proportional to the number of cells 36 . Each experiment was repeated at least three times.…”

Section: Methodsmentioning

confidence: 99%

“…The absorbance reading at 490 nm of the resulting formazan solution is proportional to the number of cells. 36 Each experiment was repeated at least three times.…”

Section: Cell Adhesion Characterizationmentioning

confidence: 99%

A novel foaming technique to develop functional open‐cell polylactic acid scaffolds for bone tissue engineering

Osman

Virgilio

Rouabhia

et al. 2023

J of Applied Polymer Sci

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The aim of this study was to develop a solvent-free polylactic acid (PLA) opencell porous scaffold for bone tissue engineering using compression molding and a new chemical foaming compound, that we named CFCO. The latter is composed of a blend of PLA and azodicarbonamide (ADA) as a chemical foaming agent, with the addition of chitosan-grafted PLA (CS-g-PLA) copolymer at various concentrations. The novelty of this approach is that during the foaming, that is, during the decomposition of the ADA foaming agent, the CS-g-PLA copolymer is projected toward the surface of the pores and strongly adhere there, which was confirmed by scanning electron microscopy and confocal microscopy. The results showed that at 6.90 wt% of CS-g-PLA copolymer, the immobilization of the latter on the surface of the pores led to a 52% increase in cell proliferation compared to the pure PLA control sample and a 26% increase compared to scaffolds that had 10 wt% of CS-g-PLA copolymer dispersed throughout the entire PLA matrix. K E Y W O R D S chemical foaming compound (CFCO), chitosan-g-PLA copolymer, open-cell porous scaffold, osteoblast, osteoblast cell adhesion and proliferation

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“…To determine whether PAC modulates DNA repair pathways, real-time PCR was performed using RT 2 Profiler PCR Array Human DNA Repair (PAHS-042Z) from Qiagen according to manufacturer instructions [70,71]. In addition, the PCR Array is a 96-well plate containing primers for a set of 84 DNA repair-related genes, five housekeeping genes, and three controls for the various ones included that have been investigated.…”

Section: Screening With Rt 2 Profiler Pcr Arraymentioning

confidence: 99%

The Curcumin Analog PAC Is a Potential Solution for the Treatment of Triple-Negative Breast Cancer by Modulating the Gene Expression of DNA Repair Pathways

Almalki,

Al-Amri,

Alrashed

et al. 2023

IJMS

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Breast Cancer (BC) is one of the most common and challenging cancers among females worldwide. Conventional treatments for oral cancer rely on the use of radiology and surgery accompanied by chemotherapy. Chemotherapy presents many side effects, and the cells often develop resistance to this chemotherapy. It will be urgent to adopt alternative or complementary treatment strategies that are new and more effective without these negative effects to improve the well-being of patients. A substantial number of epidemiological and experimental studies reported that many compounds are derived from natural products such as curcumin and their analogs, which have a great deal of beneficial anti-BC activity by inducing apoptosis, inhibiting cell proliferation, migration, and metastasis, modulating cancer-related pathways, and sensitizing cells to radiotherapy and chemotherapy. In the present study, we investigated the effect of the curcumin-analog PAC on DNA repair pathways in MCF-7 and MDA-MB-231 human breast-cancer cell lines. These pathways are crucial for genome maintenance and cancer prevention. MCF-7 and MDA-MB-231 cells were exposed to PAC at 10 µM. MTT and LDH assays were conducted to evaluate the effects of PAC on cell proliferation and cytotoxicity. Apoptosis was assessed in breast cancer cell lines using flow cytometry with annexin/Pi assay. The expression of proapoptotic and antiapoptotic genes was determined by RT-PCR to see if PAC is active in programming cell death. Additionally, DNA repair signaling pathways were analyzed by PCR arrays focusing on genes being related and confirmed by quantitative PCR. PAC significantly inhibited breast-cancer cell proliferation in a time-dependent manner, more on MDA-MB-231 triple-negative breast cancer cells. The flow cytometry results showed an increase in apoptotic activity. These data have been established by the gene expression and indicate that PAC-induced apoptosis by an increased Bax and decreased Bcl-2 expression. Moreover, PAC affected multiple genes involved in the DNA repair pathways occurring in both cell lines (MCF-7 and MDA-MB231). In addition, our results suggest that PAC upregulated more than twice 16 genes (ERCC1, ERCC2, PNKP, POLL, MPG, NEIL2, NTHL1, SMUG1, RAD51D, RAD54L, RFC1, TOP3A, XRCC3, XRCC6BP1, FEN1, and TREX1) in MDA-MB-231, 6 genes (ERCC1, LIG1, PNKP, UNG, MPG, and RAD54L) in MCF-7, and 4 genes (ERCC1, PNKP, MPG, and RAD54L) in the two cell lines. In silico analysis of gene–gene interaction shows that there are common genes between MCF-7 and MDA-MB-321 having direct and indirect effects, among them via coexpression, genetic interactions, pathways, predicted and physical interactions, and shared protein domains with predicted associated genes indicating they are more likely to be functionally related. Our data show that PAC increases involvement of multiple genes in a DNA repair pathway, this certainly can open a new perspective in breast-cancer treatment.

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Cannabis smoke condensate induces human gingival epithelial cell damage through apoptosis, autophagy, and oxidative stress

Cited by 7 publications

References 49 publications

Cellular mechanisms mediating the anti-cancer effects of carnosol on gingiva carcinoma

Cellular mechanisms mediating the anti-cancer effects of carnosol on gingiva carcinoma

A novel foaming technique to develop functional open‐cell polylactic acid scaffolds for bone tissue engineering

The Curcumin Analog PAC Is a Potential Solution for the Treatment of Triple-Negative Breast Cancer by Modulating the Gene Expression of DNA Repair Pathways

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