Cantilever Spring-Constant Calibration in Atomic Force Microscopy

Cumpson, Peter J.; Clifford, Charles A.; Portolés, José F.; Johnstone, J. E.; Munz, Martin

doi:10.1007/978-3-540-74080-3_8

Cited by 14 publications

(12 citation statements)

References 73 publications

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“…According to equation (2), two contributions to the relative measurement error should be taken into account: calibration error and error in determining the slope ratio. It was shown in (16)(17)(18) that, on applying Sader's algorithm, the calibration error σ k C /k C may reach a value of 20%. The error in measuring the slope ratio, σ /S 0 , is substantially smaller.…”

Section: Samples and Experimental Proceduresmentioning

confidence: 98%

Nanocarbons-Induced Hardening of Ultrathin Polysiloxane Block Copolymer Films

Ankudinov

Nyapshaev

Voznyakovskii

2012

Fullerenes, Nanotubes and Carbon Nanostructures

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Ultrathin films of polysiloxane block copolymers and their composites with a C 60 modifying additive were studied by atomic force microscopy. Nanometer scale surface patterns independent of the additive concentration were revealed in the surface profiles of the films. These patterns were attributed to the spatial distribution of rigid block domains at the block copolymer surface. Reliable quantitative data on mechanical properties of the films were obtained in indentation tests with specially fabricated spherical probes with a calibrated submicrometer radius of curvature. It was found that the addition of C 60 in an amount on the order of 0.01% significantly improves the elasticity of the block copolymer surface layers. At this concentration, several fullerene molecules can be introduced into each nanodomain of rigid blocks.

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Section: Samples and Experimental Proceduresmentioning

confidence: 98%

Nanocarbons-Induced Hardening of Ultrathin Polysiloxane Block Copolymer Films

Ankudinov

Nyapshaev

Voznyakovskii

2012

Fullerenes, Nanotubes and Carbon Nanostructures

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“…[24] Apart from scanning, AFM opens up aw ider ange of experimental possibilities upon use as a" mechanical" machine;t his is technically known as force spectroscopy.I nt his case, forcedistance (or force-time) experiments are carried out. [35,36] Modification of the tip (or colloidal particle) throughw ell-defined chemistry leads to ap articulart ype of force spectroscopy (chemical force microscopy)a nd an ew form of imaging based on molecular recognition. Bending of the cantilever is determined as af unction of the displacement of the piezo scanner,w hereas the force sensed by the cantilever is calculated based on Hooke's law (the bending or deflection of the cantilever is multipliedb yt he spring constant).…”

Section: Topography Imaging and Mechanical Machinesmentioning

confidence: 99%

Atomic Force Microscopy Meets Biophysics, Bioengineering, Chemistry, and Materials Science

Toca-Herrera

2019

ChemSusChem

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Briefly, herein the use of atomic force microscopy (AFM) in the characterization of molecules and (bioengineered) materials related to chemistry, materials science, chemical engineering, and environmental science and biotechnology is reviewed. First, the basic operations of standard AFM, Kelvin probe force microscopy, electrochemical AFM, and tip‐enhanced Raman microscopy are described. Second, several applications of these techniques to the characterization of single molecules, polymers, biological membranes, films, cells, hydrogels, catalytic processes, and semiconductors are provided and discussed.

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“…The spring constant of the cantilever is generally determined by analysis of its thermal fluctuations, in much the same way as the optical tweezers use Brownian motion. Other methods exist, for a review see Cumpson et al 70 While typical cantilevers have spring constants far exceeding those of optical tweezers resulting in typical forces of order nN, softer −1 Programmed Ribosomal Frameshifting as a Force-Dependent Process cantilevers have become available allowing forces in the pN regime to be measured or applied. Furthermore, recent technical improvements reducing drift and increasing tip-sample stability to as small as 100 pm have the potential of bringing AFM to single-molecule unfolding experiments that would otherwise employ optical tweezers.…”

Section: Afmmentioning

confidence: 99%

−1 Programmed Ribosomal Frameshifting as a Force-Dependent Process

Visscher

2016

Progress in Molecular Biology and Translational Science

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-1 Programmed ribosomal frameshifting is a translational recoding event in which ribosomes slip backward along messenger RNA presumably due to increased tension disrupting the codon-anticodon interaction at the ribosome's coding site. Single-molecule physical methods and recent experiments characterizing the physical properties of mRNA's slippery sequence as well as the mechanical stability of downstream mRNA structure motifs that give rise to frameshifting are discussed. Progress in technology, experimental assays, and data analysis methods hold promise for accurate physical modeling and quantitative understanding of -1 programmed ribosomal frameshifting.

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Cantilever Spring-Constant Calibration in Atomic Force Microscopy

Cited by 14 publications

References 73 publications

Nanocarbons-Induced Hardening of Ultrathin Polysiloxane Block Copolymer Films

Nanocarbons-Induced Hardening of Ultrathin Polysiloxane Block Copolymer Films

Atomic Force Microscopy Meets Biophysics, Bioengineering, Chemistry, and Materials Science

−1 Programmed Ribosomal Frameshifting as a Force-Dependent Process

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