CaO nanoparticle enhances the seedling growth of Onobrycis viciifolia under drought stress via mannitol use

Ertuş, Mehmet Macit; Yazıcılar, Büşra

doi:10.1007/s11756-023-01313-w

Cited by 4 publications

(1 citation statement)

References 27 publications

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“…3, 4, and 5. A similar observation of Onobrychis viciifolia regeneration capacity was noted exclusively under CaO NPs treatment during drought stress (Ertuş and Yazıcılar, 2023). Positive effects of CaO NPs on chickpea (Cicer arietinum L.) growth were also reported (Gandhi et al 2021).…”

Section: Evaluation Of Altered Mtr-mir159 and Mtr-mir393 Gene Express...supporting

confidence: 52%

Mitigation of Drought Stress Effects on Alfalfa (Medicago sativa L.) Callus through CaO Nanoparticles and Graphene Oxide in Tissue Culture Conditions

Yazıcılar,

Nadaroğlu,

Alaylı

et al. 2024

Preprint

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Drought stress poses a significant threat to fertile soils worldwide, triggering profound physiological, biochemical, and molecular changes in plants that adversely impact agricultural productivity. This study explores the potential of nanotechnology, specifically Calcium Oxide Nanoparticles (CaO NPs) and Graphene Oxide (GO), to ameliorate the negative effects of drought stress on two distinct alfalfa ecotypes. Seeds from Erzurum and Konya regions were regenerated in the Murashige and Skoog (MS) medium, and ensuing callus formation was induced through 2,4-D and Kinetin. The callus samples underwent a one-month treatment with varying concentrations of mannitol (50 and 100 mM), CaO NPs, and GO (0.5 and 1.5 ppm). Results revealed a decrease in dry/wet weight with increasing mannitol concentration, contrasting with an increase in weight under CaO NPs and GO treatment. Proline, DNSA, MDA, and H2O2 exhibited proportional increases under drought stress, while CaO NPs and GO treatments mitigated these effects. Physiological and biochemical analyses identified optimal conditions for Erzurum as 50 mM mannitol/2 CaO NPs/0.5 ppm GO, and for Konya as 50 mM mannitol/0.5 ppm GO. Gene expression analysis indicated up-regulation of mtr-miR159 and mtr-miR393 with heightened drought stress, with down-regulation observed in CaO NPs and GO treatments. Scanning Electron Microscopy (SEM) and Confocal Laser Scanning Microscopy (CLSM) confirmed Ca2+ accumulation in alfalfa tissues. In conclusion, CaO NPs and GO treatments exhibited a significant reduction in the adverse effects of drought stress on alfalfa callus under tissue culture conditions. This research sheds light on the potential of nanotechnological interventions to alleviate the impact of environmental stressors on crop plants, opening avenues for sustainable agriculture in the face of changing climatic conditions. Further investigations are warranted to elucidate the underlying mechanisms and scalability of these findings for field applications.

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Section: Evaluation Of Altered Mtr-mir159 and Mtr-mir393 Gene Express...supporting

confidence: 52%

Mitigation of Drought Stress Effects on Alfalfa (Medicago sativa L.) Callus through CaO Nanoparticles and Graphene Oxide in Tissue Culture Conditions

Yazıcılar,

Nadaroğlu,

Alaylı

et al. 2024

Preprint

Self Cite

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Nano‐enabled stress‐smart agriculture: Can nanotechnology deliver drought and salinity‐smart crops?

Raza

Charagh

Salehi

et al. 2023

J of Sust Agri & Env

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Salinity and drought stress substantially decrease crop yield and superiority, directly threatening the food supply needed to meet the rising food needs of the growing total population. Nanotechnology is a step towards improving agricultural output and stress tolerance by improving the efficacy of inputs in agriculture via targeted delivery, controlled release, and enhanced solubility and adhesion while also reducing significant damage. The direct application of nanoparticles (NPs)/nanomaterials can boost the performance and effectiveness of physio‐biochemical and molecular mechanisms in plants under stress conditions, leading to advanced stress tolerance. Therefore, we presented the effects and plant responses to stress conditions, and also explored the potential of nanomaterials for improving agricultural systems, and discussed the advantages of applying NPs at various developmental stages to alleviate the negative effects of salinity and drought stress. Moreover, we feature the recent innovations in state‐of‐the‐art nanobiotechnology, specifically NP‐mediated genome editing via CRISPR/Cas system, to develop stress‐smart crops. However, further investigations are needed to unravel the role of nanobiotechnology in addressing climate change challenges in modern agricultural systems. We propose that combining nanobiotechnology, genome editing and speed breeding techniques could enable the designing of climate‐smart cultivars (particularly bred or genetically modified plant varieties) to meet the food security needs of the rising world population.

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Mitigation of drought stress effects on alfalfa (Medicago sativa L.) callus through CaO nanoparticles and graphene oxide in tissue culture conditions

YAZICILAR,

NADAROĞLU,

ALAYLI

et al. 2024

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Drought stress poses a significant threat to fertile soils worldwide, triggering profound physiological, biochemical, and molecular changes in plants that adversely impact agricultural productivity. This study explores the potential of nanotechnology, specifically Calcium Oxide Nanoparticles (CaO NPs) and Graphene Oxide (GO), to ameliorate the negative effects of drought stress on two distinct alfalfa ecotypes. Seeds from Erzurum and Konya regions were regenerated in the Murashige and Skoog (MS) medium, and ensuing callus formation was induced through 1 mg L−1 2,4-D and 1 mg L−1 kinetin MS medium. The callus samples underwent a one-month treatment with varying concentrations of mannitol (50 and 100 mM), CaO NPs, and GO (0.5 and 1.5 ppm). Results revealed a decrease in dry/wet weight with increasing mannitol concentration, contrasting with an increase in weight under CaO NPs and GO treatment. Proline, DNSA, MDA, and H2O2 exhibited proportional increases under drought stress, while CaO NPs and GO treatments mitigated these effects. Physiological and biochemical analyses identified optimal conditions for Erzurum as 50 mM mannitol/2 CaO NPs/0.5 ppm GO, and for Konya as 50 mM mannitol/0.5 ppm GO. Gene expression analysis indicated up-regulation of mtr-miR159 and mtr-miR393 with heightened drought stress, with down-regulation observed in CaO NPs and GO treatments. Scanning Electron Microscopy (SEM) and Confocal Laser Scanning Microscopy (CLSM) confirmed Ca2+ accumulation in alfalfa tissues. In conclusion, CaO NPs and GO treatments exhibited a significant reduction in the adverse effects of drought stress on alfalfa callus under tissue culture conditions. This research sheds light on the potential of nanotechnological interventions to alleviate the impact of environmental stressors on crop plants, opening avenues for sustainable agriculture in the face of changing climatic conditions. Further investigations are warranted to elucidate the underlying mechanisms and scalability of these findings for field applications.

show abstract

CaO nanoparticle enhances the seedling growth of Onobrycis viciifolia under drought stress via mannitol use

Cited by 4 publications

References 27 publications

Mitigation of Drought Stress Effects on Alfalfa (Medicago sativa L.) Callus through CaO Nanoparticles and Graphene Oxide in Tissue Culture Conditions

Mitigation of Drought Stress Effects on Alfalfa (Medicago sativa L.) Callus through CaO Nanoparticles and Graphene Oxide in Tissue Culture Conditions

Nano‐enabled stress‐smart agriculture: Can nanotechnology deliver drought and salinity‐smart crops?

Mitigation of drought stress effects on alfalfa (Medicago sativa L.) callus through CaO nanoparticles and graphene oxide in tissue culture conditions

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