CAPACIDAD BIOCONTROLADORA DE AISLAMIENTOS NATIVOS DE Trichoderma sp. CONTRA EL HONGO FITOPATÓGENO Alternaria alternata, AISLADO DE YERBA MATE (Ilex paraguariensis Saint Hil.)

Castrillo, María Lorena; Bich, Gustavo Ángel; Sioli, Gastón; Zapata, Pedro Darío; Villalba, Laura Lidia

doi:10.29393/chjaas37-26cbml50026

Cited by 6 publications

(3 citation statements)

References 21 publications

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“…Species of this genus are economically important, in part because of their mycoparasitic ability, but also as they can stimulate plant resistance, and plant growth and development which increase crop production. Among these uses, T. koningiopsis was reported as a fungal species that can be used in biocontrol and other biotechnological applications ( Samuels et al, 2006 ; Castrillo et al, 2015 ; Amerio et al, 2020 ; Castrillo et al, 2021a ).…”

Section: Introductionmentioning

confidence: 99%

Trichoderma koningiopsis (Hypocreaceae) has the smallest mitogenome of the genus Trichoderma

Castrillo,

Bich,

Amerio

et al. 2023

Front. Microbiol.

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IntroductionFungal mitogenomes exhibit remarkable variation in conformation, size, gene content, arrangement and expression, including their intergenic spacers and introns.MethodsThe complete mitochondrial genome sequence of the mycoparasitic fungus Trichoderma koningiopsis was determined using the Illumina next-generation sequencing technology. We used data from our recent Illumina NGS-based project of T. koningiopsis genome sequencing to study its mitochondrial genome. The mitogenome was assembled, annotated, and compared with other fungal mitogenomes.ResultsT. koningiopsis strain POS7 mitogenome is a circular molecule of 27,560 bp long with a GC content of 27.80%. It harbors the whole complement of the 14 conserved mitochondrial protein-coding genes (PCG) such as atp6, atp8, atp9, cox1, cox2, cox3, cob, nad1, nad2, nad3, nad4, nad4L, nad5, and nad6, also found in the same gene order to other Hypocreales. The mitogenome also contains 26 transfer RNA genes (tRNAs), 5 of them with more than one copy. Other genes also present in the assembled mitochondrial genome are a small rRNA subunit and a large rRNA subunit containing ribosomal protein S3 gene. Despite the small genome size, two introns were detected in the T. koningiopsis POS7 mitogenome, one of them in cox3 gene and the other in rnl gene, accounting 7.34% of this mitogenome with a total size of 2,024 bp. A phylogenetic analysis was done using the 14 PCGs genes of T. koningiopsis strain POS7 mitogenome to compare them with those from other fungi of the Subphyla Pezizomycotina and Saccharomycotina. T. koningiopsis strain POS7 was clustered together with other representatives of Trichoderma lineage, within the Hypocreales group, which is also supported by previous phylogenetic studies based on nuclear markers.DiscussionThe mitochondrial genome of T. koningiopsis POS7 will allow further investigations into the taxonomy, phylogenetics, conservation genetics, and evolutionary biology of this important genus as well as other closely related species.

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Section: Introductionmentioning

confidence: 99%

Trichoderma koningiopsis (Hypocreaceae) has the smallest mitogenome of the genus Trichoderma

Castrillo,

Bich,

Amerio

et al. 2023

Front. Microbiol.

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“…Alternaria alternata is characterized by causing an accelerated infection (Mata-Santoyo et al, 2018). With a temperature between 28 and 30 °C and relative humidity greater than 80%, the infection can begin or worsen in approximately 4 hours (Castrillo et al, 2021). The spores of A. alternata are dispersed mainly by wind or rain splash, and these spores can overwinter among crop residues until conditions are favorable for germination (Abbas et al, 2021).…”

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confidence: 99%

“…Plants synthesize phytochemicals, such as phytoanticipins and phytoalexins, that play an important role on plant growth and reproduction, esta entre 28-30 °C y la humedad relativa mayor a 80%, esta infección puede comenzar o agravarse en un tiempo aproximado de 4 h (Castrillo et al, 2021). La diseminación de las esporas de A. alternata ocurre principalmente por el viento o por salpicaduras de lluvia, y estas esporas pueden sobrevivir el invierno en residuos de cultivos, hasta que las condiciones son favorables para germinar (Abbas et al, 2021).…”

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Polyphenols extraction from creosote bush, tarbush, and soursop using ultrasound-microwave and their effect against Alternaria alternata and Fusarium solani

Castillo¹,

León-Juárez²,

Nery‐Flores³

et al. 2022

RMF

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Crops are affected by different pathogens that cause damage to the plant and production losses. Quarantine restrictions may also occur due to the presence of a pathogen. In most cases, synthetic chemical products are used to control phytopathogens. However, some of these products have been associated with risks to human health or the environment. Therefore, it is necessary to search for more sustainable alternatives to control these pathogens. This research was carried out under the following objectives: 1. To extract polyphenols from Creosote bush, Tarbush and Soursop leaves using: different mass/volume ratios (m/v), different concentrations of ethanol, simultaneous ultrasound-microwave and 2. To determine the effect of these polyphenols against Fusarium solani and Alternaria alternata. Polyphenols were obtained from Creosote bush, Tarbush and Soursop leaves by combining ultrasound-microwave, and using different massvolume ratios and ethanol percentages. The group of polyphenols (CD) obtained with a mass/volume (m/v) ratio of 1:8 and 70% ethanol from Creosote bush leaves and the group of polyphenols (CA) obtained with a m/v ratio 1:16 and 70% ethanol from Tarbush leaves were the most effective against Fusarium solani, inhibiting 90.4 and 60.67%, respectively. For Alternaria alternata, no differences were found in the mycelial inhibition by the different groups of evaluated polyphenols, with inhibition values between 50-60% in comparison to the negative control (without polyphenols). In the profile of CD polyphenols group obtained from Creosote bush, different polyphenolic compounds reported to have antimicrobial activity were found, among which the following stand out: epirosmanol, rosmadiol, apigenin, catechin, and procyanidin C1. Polyphenolic compounds isolated from Creosote bush, Tarbush, and Soursop leaves using environmentally friendly methods (ultrasoundmicrowave) and solvents (water and ethanol) showed antifungal activity against F. solani and A. alternata.

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Fungal Diseases in Yerba Mate: Status and Management Strategies

Vereschuk,

Alvarenga,

Zapata

2024

Curr Microbiol

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CAPACIDAD BIOCONTROLADORA DE AISLAMIENTOS NATIVOS DE Trichoderma sp. CONTRA EL HONGO FITOPATÓGENO Alternaria alternata, AISLADO DE YERBA MATE (Ilex paraguariensis Saint Hil.)

Cited by 6 publications

References 21 publications

Trichoderma koningiopsis (Hypocreaceae) has the smallest mitogenome of the genus Trichoderma

Trichoderma koningiopsis (Hypocreaceae) has the smallest mitogenome of the genus Trichoderma

Polyphenols extraction from creosote bush, tarbush, and soursop using ultrasound-microwave and their effect against Alternaria alternata and Fusarium solani

Fungal Diseases in Yerba Mate: Status and Management Strategies

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