Capacitative Ca2+ entry (CCE) induced by luminal and basolateral ATP in polarised MDCK-C7 cells is restricted to the basolateral membrane

Stimulation of Cl− Secretion via Membrane-restricted Ca2+ Signaling Mediated by P2Y Receptors in Polarized Epithelia

“…It is interesting that similar results were also found in polarized MDCK-C7 cells in which the Ca 2ϩ influx, which could be activated by extracellular ATP or thapsigargin, was exclusively located in the basolateral membrane (39). Recently, it has been suggested that the localization of CCE in the basolateral membrane is a more general characteristic of all polarized epithelial tissues.…”

TRP Channels as Drug Targets

“…It has now been done by two di¡erent prominent groups, and they get completely opposite results (Paradiso et al 1995, Gordjani et al 1997. Paradiso et al (1995) got in£ux from both membranes and Gordjani et al (1997) have similar results to yours. The story might depend on culture conditions and so on.…”

“…Muallem: With the work from Gordjani et al (1997), no matter how they tried to deplete the stores they could never get Ca 2+ in£ux across the apical membrane.…”

Modulation of Cytosolic Ca 2+ Concentration in Airway Epithelial Cells by Pseudomonas aeruginosa

“…This release is followed by a sustained plateau, usually in the range 200 to 500 nM, resulting from Ca entry from the extracellular solution through Ca channels in the plasma membrane that open when the endoplasmic reticulum stores are depleted (store-operated Ca channels). Store-operated Ca channels are present in the basolateral membranes of some epithelial cells (22) and perhaps also the apical membrane (49); they are blocked by La 3ϩ (46,47). The slow and sustained P. aeruginosa-induced increase in Ca i was consistent with entry of Ca from outside the cell through store-operated Ca channels (46).…”