Capacitive Field‐Effect Biosensor Applied for the Detection of Acetoin in Alcoholic Beverages and Fermentation Broths

Jablonski, Melanie; Münstermann, Felix; Nork, Jasmina; Molinnus, Denise; Muschallik, Lukas; Bongaerts, Johannes; Wagner, Torsten; Keusgen, Michael; Siegert, Petra; Schöning, Michael J.

doi:10.1002/pssa.202000765

Cited by 9 publications

(6 citation statements)

References 38 publications

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“…A further interesting approach for such actuator-sensing platform lies in the field of enantioselective enzymes which catalyse multiple reactions (e.g., acetoin reductase for acetoin and diacetyl determination [34,35]). Here, local pH variations triggered by the LAE could shift the pH optima corresponding to the respective substrate molecule of interest.…”

Section: Discussionmentioning

confidence: 99%

Light-Addressable Actuator-Sensor Platform for Monitoring and Manipulation of pH Gradients in Microfluidics: A Case Study with the Enzyme Penicillinase

et al. 2021

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The feasibility of light-addressed detection and manipulation of pH gradients inside an electrochemical microfluidic cell was studied. Local pH changes, induced by a light-addressable electrode (LAE), were detected using a light-addressable potentiometric sensor (LAPS) with different measurement modes representing an actuator-sensor system. Biosensor functionality was examined depending on locally induced pH gradients with the help of the model enzyme penicillinase, which had been immobilized in the microfluidic channel. The surface morphology of the LAE and enzyme-functionalized LAPS was studied by scanning electron microscopy. Furthermore, the penicillin sensitivity of the LAPS inside the microfluidic channel was determined with regard to the analyte’s pH influence on the enzymatic reaction rate. In a final experiment, the LAE-controlled pH inhibition of the enzyme activity was monitored by the LAPS.

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Section: Discussionmentioning

confidence: 99%

Light-Addressable Actuator-Sensor Platform for Monitoring and Manipulation of pH Gradients in Microfluidics: A Case Study with the Enzyme Penicillinase

et al. 2021

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“…Between the fourth and ninth measurement (at day 10 and day 72), the TMV-assisted acetoin EISCAPs showed a slightly fluctuating remaining acetoin sensitivity of 46-58%. This long-term stability of at least 72 days represents a substantial improvement over acetoin biosensors where the enzyme was immobilized via crosslinking, which showed a long-term stability of 5 days [13] and 21 days [14], respectively.…”

Section: Acetoin Detection With Tmv-assisted Eiscapsmentioning

confidence: 96%

“…The application of a biosensor for the on-site detection of acetoin and diacetyl with a fast response time could circumvent these challenges. Recently, we introduced a capacitive field-effect biosensor for the detection of acetoin [13] and characterized this biosensor using real samples [14]. Here, the enzyme acetoin reductase (AR), known as (R,R)-butane-2,3-diol dehydrogenase, from Alkalihalobacillus clausii DSM 8716 T [15] was immobilized on the transducer surface of a capacitive electrolyte-insulator-semiconductor (EISCAP) sensor by means of cross-linking.…”

Section: Introductionmentioning

confidence: 99%

Detection of Acetoin and Diacetyl by a Tobacco Mosaic Virus-Assisted Field-Effect Biosensor

Welden

Severins

Poghossian³

et al. 2022

Chemosensors

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Acetoin and diacetyl have a major impact on the flavor of alcoholic beverages such as wine or beer. Therefore, their measurement is important during the fermentation process. Until now, gas chromatographic techniques have typically been applied; however, these require expensive laboratory equipment and trained staff, and do not allow for online monitoring. In this work, a capacitive electrolyte–insulator–semiconductor sensor modified with tobacco mosaic virus (TMV) particles as enzyme nanocarriers for the detection of acetoin and diacetyl is presented. The enzyme acetoin reductase from Alkalihalobacillus clausii DSM 8716T is immobilized via biotin–streptavidin affinity, binding to the surface of the TMV particles. The TMV-assisted biosensor is electrochemically characterized by means of leakage–current, capacitance–voltage, and constant capacitance measurements. In this paper, the novel biosensor is studied regarding its sensitivity and long-term stability in buffer solution. Moreover, the TMV-assisted capacitive field-effect sensor is applied for the detection of diacetyl for the first time. The measurement of acetoin and diacetyl with the same sensor setup is demonstrated. Finally, the successive detection of acetoin and diacetyl in buffer and in diluted beer is studied by tuning the sensitivity of the biosensor using the pH value of the measurement solution.

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“…They have been implemented for detecting various biochemical species such as ions [ 11 , 12 , 13 , 14 ], charged molecules (DNA (deoxyribonucleic acid) [ 15 , 16 , 17 ], protein biomarkers [ 18 , 19 , 20 , 21 , 22 ], polyelectrolytes [ 23 , 24 ]), virus-like particles [ 25 , 26 ], ligand-stabilized nanoparticles [ 27 , 28 ], etc. In addition, numerous enzyme-modified EISCAP biosensors were constructed for the detection of various analytes such as glucose [ 29 , 30 , 31 ], urea [ 30 , 31 , 32 ], creatinine [ 33 ], penicillin [ 31 , 34 ], formaldehyde [ 35 ], triglycerides [ 36 ], and acetoin [ 37 ]. The operation mechanism of these biosensors is based on the detection of local pH changes resulting from the catalytic reaction of the immobilized enzyme on the sensor surface with its specific substrate [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Towards Multi-Analyte Detection with Field-Effect Capacitors Modified with Tobacco Mosaic Virus Bioparticles as Enzyme Nanocarriers

Welden

Poghossian²,

Vahidpour

et al. 2022

Biosensors

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Utilizing an appropriate enzyme immobilization strategy is crucial for designing enzyme-based biosensors. Plant virus-like particles represent ideal nanoscaffolds for an extremely dense and precise immobilization of enzymes, due to their regular shape, high surface-to-volume ratio and high density of surface binding sites. In the present work, tobacco mosaic virus (TMV) particles were applied for the co-immobilization of penicillinase and urease onto the gate surface of a field-effect electrolyte-insulator-semiconductor capacitor (EISCAP) with a p-Si-SiO2-Ta2O5 layer structure for the sequential detection of penicillin and urea. The TMV-assisted bi-enzyme EISCAP biosensor exhibited a high urea and penicillin sensitivity of 54 and 85 mV/dec, respectively, in the concentration range of 0.1–3 mM. For comparison, the characteristics of single-enzyme EISCAP biosensors modified with TMV particles immobilized with either penicillinase or urease were also investigated. The surface morphology of the TMV-modified Ta2O5-gate was analyzed by scanning electron microscopy. Additionally, the bi-enzyme EISCAP was applied to mimic an XOR (Exclusive OR) enzyme logic gate.

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Capacitive Field‐Effect Biosensor Applied for the Detection of Acetoin in Alcoholic Beverages and Fermentation Broths

Cited by 9 publications

References 38 publications

Light-Addressable Actuator-Sensor Platform for Monitoring and Manipulation of pH Gradients in Microfluidics: A Case Study with the Enzyme Penicillinase

Light-Addressable Actuator-Sensor Platform for Monitoring and Manipulation of pH Gradients in Microfluidics: A Case Study with the Enzyme Penicillinase

Detection of Acetoin and Diacetyl by a Tobacco Mosaic Virus-Assisted Field-Effect Biosensor

Towards Multi-Analyte Detection with Field-Effect Capacitors Modified with Tobacco Mosaic Virus Bioparticles as Enzyme Nanocarriers

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