Sweat analysis is a non-invasive, simple, and convenient means of disease detection, which is extremely useful in human daily supervision. This work demonstrates the design of the nanosilica biosensor for simple, sensitive, and low-cost colorimetric sensing of glucose and pH in sweat. The nanosilica biosensing interface was prepared by chemical vapor deposition, surface modification, superwettable patterning, and sensing regions functionalization. The nanosilica substrate has high mechanical robustness and is stable enough as a biosensing platform. Colorimetric tests enable direct naked-eye observation monitoring of sweat indicators like pH and glucose without the need for electrical or optical apparatus. This will greatly enhance the convenience and reduce costs. The design of a biosensing platform will be made possible by the nanosilica biosensor, which also has significant promise for applications in clinical detection and multicomponent biosensing.