capCLIP: a new tool to probe translational control in human cells through capture and identification of the eIF4E–mRNA interactome

Abstract

Translation of eukaryotic mRNAs begins with binding of their m7G cap to eIF4E, followed by recruitment of other translation initiation factor proteins. We describe capCLIP, a novel method to comprehensively capture and quantify the eIF4E (eukaryotic initiation factor 4E) ‘cap-ome’ and apply it to examine the biological consequences of eIF4E–cap binding in distinct cellular contexts. First, we use capCLIP to identify the eIF4E cap-omes in human cells with/without the mTORC1 (mechanistic target of rapamycin, complex 1) inhibitor rapamycin, there being an emerging consensus that rapamycin inhibits translation of TOP (terminal oligopyrimidine) mRNAs by displacing eIF4E from their caps. capCLIP reveals that the representation of TOP mRNAs in the cap-ome is indeed systematically reduced by rapamycin, thus validating our new methodology. capCLIP also refines the requirements for a functional TOP sequence. Second, we apply capCLIP to probe the consequences of phosphorylation of eIF4E. We show eIF4E phosphorylation reduces overall eIF4E–mRNA association and, strikingly, causes preferential dissociation of mRNAs with short 5′-UTRs. capCLIP is a valuable new tool to probe the function of eIF4E and of other cap-binding proteins such as eIF4E2/eIF4E3.

MNK2 deficiency potentiates β-cell regeneration via translational regulation

“…10e,f ), a feature underlying 5′ UTR structures. Therefore, both CID661578 and cercosporamide modulate mRNA translation in a selective fashion where the hypertranslated mRNAs had 5′ UTRs with low GC content and the hypotranslated mRNAs had 5′ UTRs with high GC content, consistent with previous studies on eIF4E-regulated translation 23 , 24 .…”

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