Capillary electrochromatographic analysis of barbiturates in serum

Abstract

A capillary electrochromatographic method was developed for the separation of barbiturates. The separation was optimized in a 75 microm ID capillary, packed with 3-(1,8-naphthalimido)propyl-modified silyl silica gel (NAIP), studying the effect of buffer pH, buffer concentration, and mobile phase composition. Using an applied voltage of 20 kV and the short-end injection method (9 cm capillary effective length), the mobile phase of 1.0 mM citrate buffer (pH 5.0) containing 40% methanol provided the baseline separation of barbital, phenobarbital, secobarbital, and thiopental (internal standard) in less than 4.5 min. The method was successfully applied to the analysis of barbiturates in human serum. Under the optimal conditions, good repeatability and linearity were obtained in the range of 2.90-43.29 microg/mL for barbital, phenobarbital, and secobarbital.

In-line Solid-phase Extraction–Capillary Zone Electrophoresis for the Determination of Barbiturate Drugs in Human Urine

“…In the literature, different techniques, such as LC, GC and CE, have been used for the determination of barbiturate drugs in biological samples, [14][15][16][17][18][19][20][21][22]26,27 as summarized in Table 2. In these papers, samples such as urine, 14,16,19,21,26,27 plasma, 15 serum, 22 meat 17,18 and meconium 20 were analysed.…”

“…In these papers, samples such as urine, 14,16,19,21,26,27 plasma, 15 serum, 22 meat 17,18 and meconium 20 were analysed. As can be observed from the table, the LODs obtained in our study are generally lower than those reported in other papers that focused on the determination of barbiturate drugs in urine samples by CE, except for the case of Kadi et al 26 and Botello et al 27 who reported similar LODs.…”

“…The potential of CE for the determination of barbiturates in biological samples has also been demonstrated by some authors. [19][20][21][22][23][24][25][26][27] For example, Jiang et al 19 reported on the determination of barbiturates in urine by CE using a dynamically coated capillary with polycationic polymers. The LODs for standard samples were in the range of 870 to 3500 ng/mL.…”

Recent applications in capillary electrochromatography

“…At the 100 mM level the recovery of the drugs from human urine samples was better than 82%. A naphthyl-modified silica stationary phase (5 mm particles) was used by Ohyama et al [62] for the CEC separation of barbiturates. The structure of the stationary phase was similar to that prepared by the same group, shown in Fig.…”

Investigation of the novel mixed‐mode stationary phase for capillary electrochromatography. II. Separation of amino acids and peptides on sulfonated naphthalimido‐modified silyl silica gel

“…The modification ratio of 3-aminopropyl siliyl silica gel with 4-sulfo-1,8-naphthalic anhydride was 0.523 mmol/g which was estimated from the percentage of carbon by elemental analysis. CEC columns packed with SNAIP were prepared by a slurry-packing technique as reported previously [28,29]. All columns were 37 cm long with a packed length of 9 cm.…”