Capillary electrophoresis analysis of mangiferin extracted from Mangifera indica L. bark and Mangifera persiciformis C.Y. Wu et T.L. Ming leaves

Nong, Chaozan; He, Weisheng; Fleming, Debbi; Pan, Lili; Huang, Huayi

doi:10.1016/j.jchromb.2005.09.006

Cited by 25 publications

(18 citation statements)

References 37 publications

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“…The concentration was measured by CZEP capillary zone electrophoresis as described previously (Nong et al, 2005). Mangiferin was dissolved in dimethyl sulfoxide (Sigma, USA) and filtered through 0.45 μm syringe filters (Millipore, USA).…”

Section: Methodsmentioning

confidence: 99%

Mangiferin Induces Apoptosis by Regulating Bcl-2 and Bax Expression in the CNE2 Nasopharyngeal Carcinoma Cell Line

Pan¹,

Wang²,

Huang³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Section: Methodsmentioning

confidence: 99%

Mangiferin Induces Apoptosis by Regulating Bcl-2 and Bax Expression in the CNE2 Nasopharyngeal Carcinoma Cell Line

Pan¹,

Wang²,

Huang³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…[11][12][13] Several methods have been reported for determination of mangiferin, viz., high performance thin layer chromatography (HPTLC), high performance liquid chromatography (HPLC), ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS), capillary electrophoresis-mass spectroscopy (CE)-MS, and liquid chromatography-mass spectroscopy (LC)-MS from different sources. [14][15][16][17][18][19] Presence of mangiferin in S. chinensis has been reported earlier. [1,16,20] However, these studies do not elaborate the amount of mangiferin present and/or doesn't cover the multiple extraction techniques.…”

Section: Introductionmentioning

confidence: 97%

Optimization of Extraction Techniques and RP-HPLC Analysis of Antidiabetic and Anticancer Drug Mangiferin from Roots of ‘Saptarangi’ (Salacia chinensis L.)

Chavan¹,

Ghadage²,

Kshirsagar

et al. 2015

Journal of Liquid Chromatography & Related Technologies

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Saptarangi (Salacia chinensis L.) is a chief source of mangiferin, which has antidiabetic and anticancer properties. Extraction using continuous shaking extraction (CSE), ultrasonic extraction (UE), microwave assisted extraction (MAE), and steam bath assisted extraction (SBAE) were evaluated for extraction of mangiferin (MF) from roots of S. chinensis. The extracts were analyzed by reverse phase-high performance liquid chromatography (RP-HPLC) and Fourier transform-infra red spectroscopy (FT-IR) analysis. Operational parameters such as different solvents and exposure period were also evaluated. N,N-Dimethyl formamide (DMF 30%, v/v) extracted higher quantity of MF compared to other solvents. The results shows that the extraction of MF by SBAE was more efficient followed by MAE, CSE, and UE methods. Maximum quantity of MF (391.80 ppm) was obtained by SBAE technique and DMF (30%) as a solvent system. SBAE is the easiest and best method for screening of MF from S. chinensis. Furthermore, use of FT-IR validates the RP-HPLC technique for identification and quantification.

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“…Recent studies have found that the occurrence of leukemia is related to the dysregulation of the cell cycle (Nong et al, 2005). Due to p53 deletion, p53 mutations or G1/S check point deletion, most tumor cells are more and more dependent on the G2/M check point (Garrido et al, 2004;Muruganandan et al, 2005;Pardo-Andreu et al, 2008;Marquez et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Mangiferin, a naturally occurring flavonoid compound that can be extracted from bark and leaves of Mangifera indica L. (Nong et al, 2005) and other herbal species, has been demonstrated to possess antioxidative (Muruganandan et al, 2005;Pardo-Andreu et al, 2008;Marquez et al, 2012), anti-inflammatory (Garrido et al, 2004;Rivera et al, 2011), anti-bone resorption (Ang et al, 2011), and immunoregulatory effects with mild or non-existent side ef-fects (Chattopadhyay et al, 1987;Guha et al, 1996;Huang et al, 2001;Makare et al, 2001;Garcia et al, 2003). More interestingly, this flavonoid has been further shown to possess antiproliferative and antitumor growth properties, thus making it a potential anticancer agent in recent years (Huang et al, 2001;Rajendran et al, 2008;Garcia-Rivera et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Mangiferin induces cell cycle arrest at G2/M phase through ATR-Chk1 pathway in HL-60 leukemia cells

Peng¹,

Yao²,

Yang³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. This study aimed to determine the effect of mangiferin on the cell cycle in HL-60 leukemia cells and expression of the cell cycle-regulatory genes Wee1, Chk1 and CDC25C and to further investigate the molecular mechanisms of the antileukemic action of mangiferin. The inhibitory effect of mangiferin on HL-60 leukemia cell proliferation was determined by the MTT assay. The impact of mangiferin on the HL-60 cell cycle was evaluated by flow cytometry. After the cells were treated with different concentrations of mangiferin, the expression levels of Wee1, Chk1 and CDC25C mRNA were determined by RT-PCR, and Western blot was used to evaluate the expression levels of cdc25c, cyclin B1, and Akt proteins. The inhibition of HL-60 cell growth by mangiferin was dose-and time-dependent. After treatment for 24 h, cells in G2/M phase increased, and G2/M phase arrest appeared with increased mRNA expression of Wee1, Chk1 and CDC25C. Mangiferin inhibited Chk1 and cdc25c mRNA expression at high concentrations and induced Wee1 mRNA expression in a dose-dependent manner. It significantly inhibited ATR, Chk1, Wee1, Akt, and ERK1/2 phosphorylation but increased cdc2 and cyclin Z.G. Peng et al. 4990©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 4989-5002 (2015) B1 phosphorylation. Furthermore, mangiferin reduced cdc25c, cyclin B1, and Akt protein levels while inducing Wee1 protein expression. It also antagonized the phosphorylation effect of vanadate on ATR, and the phosphorylation effect of EGF on Wee1. These findings indicated that mangiferin inhibits cell cycle progression through the ATR-Chk1 stress response DNA damage pathway, leading to cell cycle arrest at G2/M phase in leukemia cells.

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Capillary electrophoresis analysis of mangiferin extracted from Mangifera indica L. bark and Mangifera persiciformis C.Y. Wu et T.L. Ming leaves

Cited by 25 publications

References 37 publications

Mangiferin Induces Apoptosis by Regulating Bcl-2 and Bax Expression in the CNE2 Nasopharyngeal Carcinoma Cell Line

Mangiferin Induces Apoptosis by Regulating Bcl-2 and Bax Expression in the CNE2 Nasopharyngeal Carcinoma Cell Line

Optimization of Extraction Techniques and RP-HPLC Analysis of Antidiabetic and Anticancer Drug Mangiferin from Roots of ‘Saptarangi’ (Salacia chinensis L.)

Mangiferin induces cell cycle arrest at G2/M phase through ATR-Chk1 pathway in HL-60 leukemia cells

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