Capillary electrophoresis determination of antihistamines in serum and pharmaceuticals

Rambla-Alegre, Maria; Péris-Vicente, Juan; Esteve‐Romero, Josep; Capella‐Peiró, María‐Elisa; Bose, Devasish

doi:10.1016/j.aca.2010.03.041

Cited by 25 publications

(26 citation statements)

References 22 publications

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“…This modeling allows the prediction of the retention factor, efficiency, asymmetry and resolution, as well as the drawing of simulated chromatograms. The reliability of these simulations, checked for different groups of compounds by comparing experimental and predicted retention factors [23,24,38,40,43,45,46], is confirmed by their low errors (<5%). The optimal mobile phase was selected on the basis of maximum resolution/minimum analysis times.…”

Section: Composition Of the Mobile Phasementioning

confidence: 90%

Application of Micellar Liquid Chromatography for The Determination of Antitumoral and Antiretroviral Drugs in Plasma

Péris-Vicente

Casas-Breva²,

Roca-Genovés

et al. 2014

Bioanalysis

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In micellar liquid chromatography, the mobile phase is made of a surfactant and, eventually, an alcohol. This article describes several methods to measure the concentration of antitumoral and antiretroviral drugs in plasma, utilizing micellar liquid chromatography. Samples can be injected after dilution with a micellar solution and filtration, because proteins and other endogenous compounds are solubilized in micellar medium. We will discuss the following optimized parameters: dilution ratio, type of column, detection conditions and mobile phase composition. This article will also cover the validation performed following the International Conference on Harmonization guidelines and the results reported in the literature, indicating that the methods are useful for the routine analysis of plasma samples for clinical purposes.

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Section: Composition Of the Mobile Phasementioning

confidence: 90%

Application of Micellar Liquid Chromatography for The Determination of Antitumoral and Antiretroviral Drugs in Plasma

Péris-Vicente

Casas-Breva²,

Roca-Genovés

et al. 2014

Bioanalysis

Self Cite

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“…A large number of CE‐UV analysis have been performed in pharmaceutical and herbal product applications 165–195. Most of these were applied for the separation of bioactive diterpenoids, triterpenes, flavonoids, and phenolic compounds.…”

Section: Detection Strategiesmentioning

confidence: 99%

Capillary electrophoresis of natural products: Highlights of the last five years (2006–2010)

2011

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An extensive search of the published research and review articles indicated a growing number of works that employed electroseparation techniques. Capillary zone electrophoresis (CZE) and electrokinetic chromatography (EKC), in particular, were found to be popular for the analyses of natural products. This review outlines the important developments in this field over the years, especially during the 2006-2010 period. An overview of the natural product applications such as pharmaceuticals/herbal products, fingerprinting and quality control, food and food contaminants, and toxicological compounds of interest to forensics were presented. Important areas of detection strategies, microchips, sample preconcentration, and chiral separations were also discussed. Hence, quick information was provided on the researches already undertaken and the possibilities of unexplored areas. In addition, fundamental concepts for the understanding of CZE and EKC and their suitability for natural products analyses were briefly discussed.

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“…They are administered as decongestants for the treatment of coughs, colds, and allergies [1]. Structurally, antihistamines are substituted ethylamines [2,3]. The structure can be seen in Figure 1.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, a capillary electrophoresis method has been proposed to detect antihistamines in pharmaceuticals and blood [3]. This method proposes an easy sample preparation: the solubilization of pharmaceutical formulations and serum in a micellar solution.…”

Section: Introductionmentioning

confidence: 99%

Validation of a Serum Analysis Method to Analyze Antihistamines by Capillary Electrophoresis

Péris-Vicente

Carda‐Broch

Esteve‐Romero

2014

Journal of Applied Chemistry

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The validation of an electrophoresis-based analytical method to quantify 17 antihistamines in pharmaceutical formulations and serum is described. Then, whether the methodology provides true values with low uncertainty and is able to detect the concentration range level of analyte usually found in the matrix was evaluated. The analytical method was validated following the recommendations of an official guide to provide more reliability to the results. The ICH Harmonized Tripartite Guideline was selected because it was especially developed for analysis of drugs. The guide and the following required validation parameters, selectivity, calibration range, linearity, limit of detection, limit of quantification, inter- and intraday accuracy and precision, and robustness, were described. The method was inexpensive, fast, simple, environmentally friendly, and useful for routine analysis. The methodology was successfully validated and applied to commercial pharmaceutical formulations and spiked blank serum samples.

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Capillary electrophoresis determination of antihistamines in serum and pharmaceuticals

Cited by 25 publications

References 22 publications

Application of Micellar Liquid Chromatography for The Determination of Antitumoral and Antiretroviral Drugs in Plasma

Application of Micellar Liquid Chromatography for The Determination of Antitumoral and Antiretroviral Drugs in Plasma

Capillary electrophoresis of natural products: Highlights of the last five years (2006–2010)

Validation of a Serum Analysis Method to Analyze Antihistamines by Capillary Electrophoresis

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