Capillary electrophoresis fingerprinting of 8-aminopyrene-1,3,6-trisulfonate derivatized nitrocellulose after partial acid depolymerization

Alinat, Elodie; Delaunay, Nathalie; Przybylski, Cédric; Daniel, Régis; Archer, Xavier; Gareil, Pierre

doi:10.1016/j.chroma.2015.01.092

Cited by 5 publications

(10 citation statements)

References 44 publications

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“…To this mixture were added the first six cellodextrin oligomers (DP2-DP7), which can be produced as intermediate residues, during the partial acidic depolymerization (and denitration) of nitrocellulose, according to the conditions followed, and prior to its completeness, leading to glucose [57]. Analysis of cellodextrins should also be of interest in the cellulose industry, and for the characterization of food and nonfood crops.…”

Section: Resultsmentioning

confidence: 99%

Analysis of underivatized cellodextrin oligosaccharides by capillary electrophoresis with direct photochemically induced UV‐detection

et al. 2015

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Analysis of underivatized cellodextrin oligosaccharides by capillary electrophoresis with direct photochemically induced UV-detectionThis work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples. Separation was achieved with a highly alkaline BGE containing sodium chloride and direct mid-UV-absorbance detection was performed after photo-oxidation in the detection window. EOF was reversed to speed up the analysis using a dynamic capillary coating by hexadimethrine bromide. A central composite design was carried out to determine the effects of BGE conductivity and sodium hydroxide concentration on resolutions between neighboring peaks, and analysis time. A desirability analysis on modeled responses was applied to maximize resolutions and to minimize analysis time. The simultaneous analysis in 20 min total runtime of the 15 carbohydrates plus internal reference (naphthalene sulfonate) was carried out at 25°C with a BGE composed of 77.4 mM NaOH and 183 mM NaCl to adjust the conductivity at the optimum value. Finally, the resolution robustness was checked. This new method should also be of interest to monitor food and nonfood crop products. Keywords:Cellodextrins / Capillary electrophoresis / Design of experiments / Direct UV detection / Oligosaccharide DOI 10.1002/elps.201400605Additional supporting information may be found in the online version of this article at the publisher's web-site IntroductionCarbohydrates are ubiquitous compounds in the living world, as natural and degradation products, metabolites, or conjugates. They are also widely used as food or beverage additives. This class of compounds of tremendous complexity actually encompasses a variety of molecules differing in size (mono-, oligo-, and polysaccharides), epimerization or isomerization, glycosidic linkage, branching, and functionality , and mixed modes [11,13] have all been commonly employed. Separations have been based on hydrogen bonding, size, complexing ability, and in some cases charge. As far as detection is concerned, great difficulties are encountered due to the lack of a chromophoric moiety. According to the sensitivity requirements and separation mode, different detection strategies have been implemented, namely, without-or with pre-or postcolumn

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Section: Resultsmentioning

confidence: 99%

Analysis of underivatized cellodextrin oligosaccharides by capillary electrophoresis with direct photochemically induced UV‐detection

et al. 2015

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“…The handling of minute samples is difficult, and thus this mode is applied when ample volumes of samples and reagents are available. Pre‐capillary mode was the most commonly applied procedure accounting for 2/3 or 29 of the 46 covered articles .…”

Section: Derivatisation Modesmentioning

confidence: 99%

Derivatisation for separation and detection in capillary electrophoresis (2015–2017)

Wuethrich

Quirino

2017

Electrophoresis

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Derivatisation is an integrated part of many analytical workflows to enable separation and detection of the analytes. In CE, derivatisation is adapted in the four modes of pre-capillary, in-line, in-capillary, and post-capillary derivatisation. In this review, we discuss the progress in derivatisation from February 2015 to May 2017 from multiple points of view including sections about the derivatisation modes, derivatisation to improve the analyte separation and analyte detection. The advancements in derivatisation procedures, novel reagents, and applications are covered. A table summarising the 46 reviewed articles with information about analyte, sample, derivatisation route, CE method and method sensitivity is provided.

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“…[25,26] The authors also employed the same CE methodology to detect anions and cations in consumer fireworks: after a physical characterization of the six types of consumer fireworks studied, which authors claimed to be essential for result interpretation, they were analysed and chemically characterized. [27] RSDtmig < 1.1 (n = 6) RSDArea < 3.5 (n = 6) [33] (continued on next page)…”

Section: Conventional Cementioning

confidence: 99%

“…[28,29] Finally, other studies have been carried out for the determination of intact pre-blast nitrostarch and nitrocellulose, a secondary explosive that may be present in some explosives of interest such as dynamites or home-made explosives. [30,[32][33][34][35] CE is a promising candidate for the analysis of cations and anions due to its ability to detect charged macromolecules at low concentrations, and thus can be used for collection post-blast residues at crime scene. However, matrix effects and contamination must be considered due to the prevalence of anions and cations in the environment.…”

Section: Conventional Cementioning

confidence: 99%

“…In this method, large polymeric chains were derivatized from NC, and the nitrogen content was quantified using an offline spectrometric technique (matrix-assisted laser desorption ionization time-of-flight mass spectrometry). [33] Finally, M. Calcerrada et al used the derivatization process previously described in conventional CE-LIF [30,32], but performed microstructured capillary electrophoresis (MCE) wherein the conventional separation capillary is replaced by a microstructured capillary (MSC). In the first work, two different MSCs were used in the CE equipment to carry out comparative studies with conventional capillaries of 25-and 50-μm internal diameter (id).…”

Section: Conventional Cementioning

confidence: 99%

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Recent advances in capillary electrophoresis instrumentation for the analysis of explosives

Calcerrada

González‐Herráez

García-Ruiz

2016

TrAC Trends in Analytical Chemistry

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Capillary electrophoresis fingerprinting of 8-aminopyrene-1,3,6-trisulfonate derivatized nitrocellulose after partial acid depolymerization

Cited by 5 publications

References 44 publications

Analysis of underivatized cellodextrin oligosaccharides by capillary electrophoresis with direct photochemically induced UV‐detection

Analysis of underivatized cellodextrin oligosaccharides by capillary electrophoresis with direct photochemically induced UV‐detection

Derivatisation for separation and detection in capillary electrophoresis (2015–2017)

Recent advances in capillary electrophoresis instrumentation for the analysis of explosives

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