Capillary electrophoresis for the investigation of two novel aminoalkanol derivatives of 1,7‐diethyl‐8,9‐diphenyl‐4‐azatricyclo[5.2.1.02,6] dec‐8‐ene‐3,5,10‐trione as potential anticancer drugs in water solution and serum

Abstract

A simple, rapid, capillary zone electrophoresis method was developed and validated for the analysis of two novel aminoalkanol derivatives (I) and (II) of 1,7-diethyl-8,9diphenyl-4-azatricyclo[5.2.1.0 2,6 ]dec-8-ene-3,5,10-trione, which were found in earlier studies as potential anticancer drugs. Samples were analyzed to demonstrate the specificity and stability indicating ability of the developed method. The samples were extracted using n-hexane-ethyl acetate mixture in the ratio of 90:10. Electrophoretic separation was performed on a eCAP fused silica capillary (37 cm length, 50 μm inside diameter) with a 50 mM tetraborate buffer as a background electrolyte adjusted to pH = 2.5. The separation time of (I) and (II) was achieved within 7 min. In addition, analysis of the two compounds in the serum was conducted. Limits of detection of (I) and (II) by UV absorbance at 200 nm were achieved in the range of 87.4-92.1 ng/mL.The sufficient recovery was observed in the range of 90.3-99.8%. The quantification limits for the compounds (I) and (II) were in the range of 279.71-291.03 ng/mL, respectively. The method has been successfully applied to the analysis of compounds (I) and (II) in serum samples. K E Y W O R D Santicancer drugs, capillary electrophoresis, drug monitoring 648

Kinetic Studies of Newly Patented Aminoalkanol Derivatives with Potential Anticancer Activity as Competitive Inhibitors of Prostate Acid Phosphatase

“…The synthesis, chemical characterization, and anticancer activity of the aforementioned compounds (I) and (II) were described previously in a patent application [36]. The present work is a continuation of our studies on aminoalkanol derivatives of dicarboximides [37][38][39][40][41]. In one of our previous studies, we developed a capillary electrophoresis (CE) method for analyzing the inhibition of tissue-non-specific alkaline phosphatase (TNAP) by the aminoalkanol derivatives of dimethyldicarboximide [38].…”

Catalase Inhibition by Aminoalkanol Derivatives with Potential Anti-Cancer Activity—In Vitro and In Silico Studies Using Capillary Electrophoresis Method

“…In our previous studies, we have shown that these derivatives (I, II, III, IV), apart from their primary antitumor activity, also inhibited the enzymatic activity of alkaline phosphatase [30], acid phosphatase [31] and acetylcholinesterase [32]. These derivatives were also previously investigated for their determination in biological material [33][34][35][36] and for their cytotoxicity on cancer cell lines such as K562 and HeLa cells [29]. In general, compounds with anti-cancer properties are of great interest in medicine, and their action may additionally affect the activity of many enzymes that protect against cancer.…”

In Vitro and In Silico Kinetic Studies of Patented 1,7-diEthyl and 1,7-diMethyl Aminoalkanol Derivatives as New Inhibitors of Acetylcholinesterase

“…These compounds were also investigated in our previous works on the possibility of their determination [ 38 , 39 , 40 , 41 ] and their inhibitory effect on alkaline phosphatase [ 42 ] and prostate acid phosphatase [ 43 ]. Interesting results described in our previous works [ 42 , 43 ] prompted us to conduct research on the influence of the mentioned aminoalkanol derivatives with potential anticancer activity on the enzymatic activity of acetylcholinesterase—an enzyme extremely important in our body.…”