Capillary electrophoresis for the screening and diagnosis of inherited hemoglobin disorders. Ready for prime time?

Lippi, Giuseppe; Plebani, Mario

doi:10.1515/cclm-2015-0545

Cited by 7 publications

(5 citation statements)

References 13 publications

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“…Capillary electrophoresis is an accurate instrument for screening and diagnosing hemoglobinopathies. Recently, the FDA recommended using capillary automated system even in routine laboratory diagnoses (Keren et al 2008;Lippi and Plebani 2016).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of hemolysis effect on hemoglobin measurement by capillary electrophoresis

Mokhtariye

Varasteh

Alaei³

et al. 2019

J Anal Sci Technol

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Background: Hemoglobin (Hb) is a hemeprotein with two linked pairs of globin chains. Each chain is connected to a heme residue in its center. Hemoglobinopathies are divided into quantitative and qualitative defects in globin synthesis. Hemolysis is a pre-analytical problem that reduces quality of sample for measurement of many analytes. Methods: Blood samples from 311 female and 189 male subjects with normal and abnormal Hb electrophoresis patterns were studied. Three milliliters of whole blood was obtained from all subjects and transferred into EDTA tubes. To analyze hemolysis, 1.5 ml of blood from each tube was aliquoted and frozen, and the remaining blood was stored at 4°C for 24 h. Hemoglobin was measured in both hemolyzed and non-hemolyzed samples by capillary electrophoresis. Results and discussion: Data was analyzed with linear regression. The results were linear for the lower limit of detection of 9, 0.5, and 0.1% up to at least 99.5, 6.6, and 99.4% for HbA, HbA 2 , and HbF, respectively. Method comparison demonstrated good agreement between non-hemolyzed and hemolyzed conditions for hemoglobin measurement. Conclusion: Use of hemolyzed samples had no effected on hemoglobin measurements.

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Section: Resultsmentioning

confidence: 99%

Evaluation of hemolysis effect on hemoglobin measurement by capillary electrophoresis

Mokhtariye

Varasteh

Alaei³

et al. 2019

J Anal Sci Technol

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“…However, there is no clearly defined consensus on the cut-off value of HbA2 for identifying BTT [ 23 ]. Many centers use an HbA2 value of ≥3.5% for the diagnosis of BTT, as recommended by a few authors and textbooks [ 4 , 23 ], while others have recommended a cut-off of ≥4% and documented a higher sensitivity for detecting cases of BTT at this value [ 1 , 6 , 14 , 23 , 24 ]. In the present study, we used a cut-off of 4% for HbA2 for diagnosing BTT.…”

Section: Discussionmentioning

confidence: 99%

“…These disorders occur worldwide, but there is a considerable variation in the prevalence between different regions of the world, and even between different areas within a country. The prevalence of these disorders is gradually showing an increasing trend in all parts of the world due to migratory populations [ 1 , 2 ]. β thalassemia is the most common thalassemia syndrome reported in India, along with some hemoglobinopathies being encountered primarily in certain regions/states of the country, like hemoglobin S (HbS) in Orissa, hemoglobin D (HbD) in Punjab, and hemoglobin E (HbE) in the north-eastern states [ 3 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…Thalassemia and hemoglobinopathies are one of the commonest inherited hematological disorders. According to the World Health Organization, almost 7% of the entire population of the world is a carrier for hemoglobin (Hb) disorders [1,2]. These disorders pose a major burden to the public healthcare system in many parts of the world, including India [1,[3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

“…According to the World Health Organization, almost 7% of the entire population of the world is a carrier for hemoglobin (Hb) disorders [1,2]. These disorders pose a major burden to the public healthcare system in many parts of the world, including India [1,[3][4][5][6]. The most effective approach to overcome this problem is the implementation of a robust screening program at the population level.…”

Section: Introductionmentioning

confidence: 99%

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Thalassemia and Hemoglobinopathy Screening in Women Attending Antenatal Clinic at a Tertiary Care Center in Uttarakhand, India: A Re-look at the Laboratory Parameters Mandating High-Performance Liquid Chromatography Workup

Singh¹,

Chowdhury²,

Bahadur³

et al. 2023

Cureus

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Introduction: Thalassemia and hemoglobinopathies are the most common inherited hematological disorders. Of these, β thalassemia is the commonest disorder reported in India, followed by certain hemoglobinopathies encountered in different regions of the country. The data pertaining to the incidence of these disorders in the Uttarakhand region of India are sparse. Aim and objectives: To ascertain the prevalence and spectrum of thalassemia/hemoglobinopathies amongst antenatal women in Uttarakhand. The study also aimed to analyze the ability of red cell indices in differentiating beta thalassemia trait (BTT) from mild iron deficiency anemia (IDA). Material and methods: A total of 460 pregnant women in the first trimester of pregnancy were screened by cation exchange high-performance liquid chromatography. Retention time and proportions of normal/abnormal hemoglobin peaks were documented in all cases. Hemoglobin A2 (HbA2) values of ≥4% were taken as a cut-off for diagnosing BTT. Blood samples were also collected for complete blood counts, reticulocyte counts, and serum ferritin. The ability of the various discriminatory indices to differentiate between IDA and BTT was also assessed. Results: The prevalence of BTT and hemoglobin D-Punjab trait amongst pregnant women was found to be 2.6% and 0.2%, respectively. RBC count, mean corpuscular volume (MCV), and mean corpuscular hemoglobin (MCH) were found to be moderately strong predictors of BTT, with an area under the curve of 0.860, 0.857, and 0.842, respectively, which were comparable to the discriminatory indices found to be most useful in this study. Conclusion: In view of the 2.6% prevalence of BTT in antenatal women in this region of Uttarakhand, a routine screening will be helpful in detecting carriers early in the antenatal period. Careful interpretation of red cell indices is crucial to the distinction between BTT and IDA. Discriminatory indices are reasonably accurate in differentiating BTT from mild iron deficiency, but for practical purposes, MCV and MCH provide equivalent information to identify cases that require further workup.

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Inherited disorders of hemoglobin: A review of old and new diagnostic methods

Franco

Karkoska

McGann

2024

Blood Cells, Molecules, and Diseases

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Capillary electrophoresis for the screening and diagnosis of inherited hemoglobin disorders. Ready for prime time?

Cited by 7 publications

References 13 publications

Evaluation of hemolysis effect on hemoglobin measurement by capillary electrophoresis

Evaluation of hemolysis effect on hemoglobin measurement by capillary electrophoresis

Thalassemia and Hemoglobinopathy Screening in Women Attending Antenatal Clinic at a Tertiary Care Center in Uttarakhand, India: A Re-look at the Laboratory Parameters Mandating High-Performance Liquid Chromatography Workup

Inherited disorders of hemoglobin: A review of old and new diagnostic methods

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