Capillary electrophoresis in phytochemical analysis (2014–2017)

Abstract

This updated review is focused on the applications of CE in phytochemical analysis during the period of 2014 to April 2018 (about 130 papers were included). The article mainly consists of three main sections, including sample pretreatment (extraction and on‐line pre‐concentration), method development (separation and detection) and applications in quality evaluation of medicinal plants, screening of active compounds (enzyme inhibitors) and studying interaction between phytochemicals and bio‐macromolecules.

Extraction Systems and Analytical Techniques for Food Phenolic Compounds: A Review

“…Moreover, for routine analysis and quantification, the wavelength 280 nm can be applied since most phenolic compounds show absorption at this wavelength to a greater or lesser extent [ 91 ]. Other usual absorption wavelengths are, e.g., 210 nm for phenolic compounds, 240 nm for secoiridoids, 310–320 nm for hydroxycinnamic acids, 350–370 nm for flavonols, 340–350 nm for flavones, and 520 nm for anthocyanins [ 91 , 99 , 100 , 101 ]. Nonetheless, this presents some drawbacks for identification.…”

Soluble Carbohydrates in Several Transylvanian Potato Cultivars

“…Some major drawbacks of RID such as the lack of sensitivity and the incompatibility with gradients can be compensated using other types of detection, such as evaporative light scattering detection [ 40 , 41 , 42 ], electrochemical detection [ 43 , 44 ] or mass spectrometry [ 29 , 45 , 46 ], but prices and systems’ complexity are higher. Using HPLC, non-derivatized carbohydrates are usually separated on silica-based amino columns [ 47 ]; ion chromatography can provide an alternative to such separations [ 29 , 48 , 49 , 50 ], while capillary electrophoresis offers a different approach [ 51 , 52 ]. In most cases, the soluble carbohydrate extraction was accomplished using ethanolic solutions, heating and mixing providing a higher efficiency for the extraction procedure.…”