Capillary electrophoresis in the N-glycosylation analysis of biopharmaceuticals

Guttman, András

doi:10.1016/j.trac.2013.04.006

Cited by 28 publications

(15 citation statements)

References 47 publications

(23 reference statements)

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“…Separation is then commonly achieved by hydrophilic interaction liquid chromatography (HILIC)-HPLC or UPLC [16,17], or by C(G)E with fluorescence detection [18,19]. As presented in a multilaboratory study, fluorescent labeling is the critical step in profiling due to possible incomplete derivatization, resulting in greater laboratory variability than MS-based Nglycomic methods [20].…”

Section: Current Methods In Glycosylation Analysis Of Biopharmaceuticalsmentioning

confidence: 99%

Mass spectrometry for glycosylation analysis of biopharmaceuticals

Dotz

Haselberg

Shubhakar

et al. 2015

TrAC Trends in Analytical Chemistry

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Section: Current Methods In Glycosylation Analysis Of Biopharmaceuticalsmentioning

confidence: 99%

Mass spectrometry for glycosylation analysis of biopharmaceuticals

Dotz

Haselberg

Shubhakar

et al. 2015

TrAC Trends in Analytical Chemistry

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“…. Guttman discussed all important steps for N‐glycosylation analysis of biopharmaceuticals . He described carbohydrate sequencing by exoglycosidase arrays in conjunction with CE for the identification of linkage and positional isomers and outlined a possible automated workflow.…”

Section: Analytical Applicationsmentioning

confidence: 99%

“…. Guttman demonstrated the role of CE in the N‐glycosylation analysis of biopharmaceuticals . Analytical aspects of biosimilarity issues of protein drugs were discussed in Kalman‐Szekeres et al.…”

Section: Analytical Applicationsmentioning

confidence: 99%

Recent advances in combination of capillary electrophoresis with mass spectrometry: Methodology and theory

Klepárnı́k

2014

Electrophoresis

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This review focuses on the latest development of microseparation electromigration methods in capillaries and microfluidic devices with MS detection and identification. A wide selection of 183 relevant articles covers the literature published from June 2012 till May 2014 as a continuation of the review article on the same topic by Kleparnik [Electrophoresis 2013, 34, 70-86]. Special attention is paid to the new improvements in the theory of instrumentation and methodology of MS interfacing with capillary versions of zone electrophoresis, ITP, and IEF. Ionization methods in MS include ESI, MALDI, and ICP. Although the main attention is paid to the development of instrumentation and methodology, representative examples illustrate also applications in the proteomics, glycomics, metabolomics, biomarker research, forensics, pharmacology, food analysis, and single-cell analysis. The combinations of MS with capillary versions of electrochromatography and micellar electrokinetic chromatography are not included.

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“…For the analysis of biopharmaceuticals the so called CE-SDS, which refers to CGE replacing the classical SDS-PAGE, and cIEF have been used for protein characterization [1,2,9,[16][17][18]. CE-MS has become a routine method for small molecule analysis [19][20][21] and an integral part of glycan analysis of biopharmaceuticals [22][23][24][25][26][27] and natural glycoproteins [27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Recent advances in capillary electrophoretic migration techniques for pharmaceutical analysis (2013–2015)

et al. 2016

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This review updates and follows-up a previous review by highlighting recent advancements regarding capillary electromigration methodologies and applications in pharmaceutical analysis. General approaches such as quality by design as well as sample injection methods and detection sensitivity are discussed. The separation and analysis of drug-related substances, chiral CE, and chiral CE-MS in addition to the determination of physicochemical constants are addressed. The advantages of applying affinity capillary electrophoresis in studying receptor-ligand interactions are highlighted. Finally, current aspects related to the analysis of biopharmaceuticals are reviewed. The present review covers the literature between January 2013 and December 2015.

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Capillary electrophoresis in the N-glycosylation analysis of biopharmaceuticals

Cited by 28 publications

References 47 publications

Mass spectrometry for glycosylation analysis of biopharmaceuticals

Mass spectrometry for glycosylation analysis of biopharmaceuticals

Recent advances in combination of capillary electrophoresis with mass spectrometry: Methodology and theory

Recent advances in capillary electrophoretic migration techniques for pharmaceutical analysis (2013–2015)

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