Capillary Electrophoresis Method Validation for Organic Acids Assessment in Probiotics

Gatea, Florentina; Teodor, Eugenia Dumitra; Păun, Gabriela; Matei, Alina; Radu, Gabriel Lucian

doi:10.1007/s12161-014-0018-1

Cited by 12 publications

(9 citation statements)

References 20 publications

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“…The antioxidant potential registered after products addition was confirmed by previous studies and was primarily correlated with the number of phenolic compounds ( R 2 > 0.7) [ 14 ]. Considering one of the purposes of this study, it could be argued that the antioxidant potential demonstrated in vitro was similar to that expressed by the hydroalcoholic extracts from the fruiting body of the same species [ 15 ].…”

Section: Discussionsupporting

confidence: 79%

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In Vitro Ecological Response of the Human Gut Microbiome to Bioactive Extracts from Edible Wild Mushrooms

2018

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This study presents the effect of two new products based on atomized extracts from edible wild mushrooms (RoBioMush1, RoBioMush2) on the microbiota of three target groups: clinically healthy (NG) individuals, individuals with nutritional disorders (ND), and individuals with cardiovascular diseases (CVD). The microbiota fingerprints were determined by quantitative polymerase chain reaction (qPCR). Modulations in the simulated microbiome were established and correlated with the presence of phenolic compounds released in the in vitro environment (a three-stage culture system GIS2 simulator, ). The high metabolizing capacity of NG and CVD correlated positively with the rest of the biological activities expressed in vitro. ND microbiota consumed a wide spectrum of monosaccharides from the products. Xylose was present in large quantities in the descending segment (minimum: 175 μg/mL for ND). The primary conclusion was that the microbiological ecosystem was modulated, as proven by the presence of specific biomarkers (e.g., ammonium levels and fingerprints of short-chain fatty acids–SCFAs), which stimulate the organism’s health status and were correlated with the restoration of a normal microbiota fingerprint.

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Section: Discussionsupporting

confidence: 79%

“…An Agilent CE instrument (ChemStation software) with a diode array detector (DAD) was used to partition the analytes [ 14 ]. Separation was achieved on a standard bare fused silica capillary (Agilent Technologies, Santa Clara, CA, USA) with an internal diameter of 50 μm and an effective length of 72 cm.…”

Section: Methodsmentioning

confidence: 99%

In Vitro Ecological Response of the Human Gut Microbiome to Bioactive Extracts from Edible Wild Mushrooms

2018

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“…Besides, organic acids can be used as indicators of some food processes such as the bacterial activity in food fermentation. For example, a simple and fast CE method has been developed for the separation and quantification of nine aliphatic and three aromatic organic acids in probiotic products of lactic acid bacteria with LOD values from 0.001‐1.43 μg/mL and LOQ values from 0.004 to 4.72 μg/mL in a simple, rapid and reliable analysis . Eight representative organic acids from rice wine and beer, including oxalic, tartaric, formic, citric, malic, lactic, succinic and acetic acids, were analyzed and quantified using a direct injection of the sample with a simple dilution in a CE instrument with indirect UV detection.…”

Section: Small Organic and Inorganic Compoundsmentioning

confidence: 99%

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

et al. 2017

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Recent advances in the application of capillary electromigration methods for food analysis and FoodomicsThis review work presents and discusses the main applications of capillary electromigration methods in food analysis and Foodomics. Papers that were published during the period February 2015-February 2017 are included following the previous review by Acunha et al. (Electrophoresis 2016, 37, 111-141). The paper shows the large variety of food related molecules that have been analyzed by CE including amino acids, biogenic amines, carbohydrates, chiral compounds, contaminants, DNAs, food additives, heterocyclic amines, lipids, peptides, pesticides, phenols, pigments, polyphenols, proteins, residues, toxins, vitamins, small organic and inorganic compounds, as well as other minor compounds. This work describes the last results on food quality and safety, nutritional value, storage, bioactivity, as well as uses of CE for monitoring food interactions and food processing including recent microchips developments and new applications of CE in Foodomics.

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“…A simple CE method has also been partially validated for the simultaneous quantification of nine aliphatic (formic, oxalic, succinic, malic, tartaric, acetic, citric, lactic, and butyric) and three aromatic (benzoic, phenyllactic, and hydroxyphenyllactic) organic acids in 15 min in fermentation products of lactic acid bacteria . The optimum BGE composition was 0.5 M H 3 PO 4 and 0.5 mM CTAB (pH 6.24) with 15% v/v methanol.…”

Section: Small Organic and Inorganic Compoundsmentioning

confidence: 99%

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

et al. 2015

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This review work presents and discusses the main applications of capillary electromigration methods in food analysis and Foodomics. Papers that were published during the period February 2013-February 2015 are included following the previous review by Garcia-Cañas et al. (Electrophoresis, 2014, 35, 147-169). Analysis by CE of a large variety of food-related molecules with different chemical properties, including amino acids, hazardous amines, peptides, proteins, phenols, polyphenols, lipids, carbohydrates, DNAs, vitamins, toxins, contaminants, pesticides, residues, food additives, as well as small organic and inorganic compounds. This work includes recent results on food quality and safety, nutritional value, storage, bioactivity, as well as applications of CE for monitoring food processing. The use, among other CE developments, of microchips, CE-MS, and chiral CE in food analysis and Foodomics is also discussed.

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Capillary Electrophoresis Method Validation for Organic Acids Assessment in Probiotics

Cited by 12 publications

References 20 publications

In Vitro Ecological Response of the Human Gut Microbiome to Bioactive Extracts from Edible Wild Mushrooms

In Vitro Ecological Response of the Human Gut Microbiome to Bioactive Extracts from Edible Wild Mushrooms

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

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