Capillary electrophoresis of bone marrow stromal cells with uptake of heparin‐functionalized poly(lactide‐co‐glycolide) nanoparticles during differentiation towards neurons

Abstract

This study analyzes the varying electrophoretic mobility and zeta potential of bone marrow stromal cells (BMSCs) during their differentiation towards neurons. Electrophoresis of primary BMSCs and neuron growth factor (NGF)-induced neuron-like cells with the uptake of heparin-functionalized poly(lactide-co-glycolide) (PLGA) nanoparticles (NPs) are also investigated. Immunofluorescent images revealed that a high concentration of NGF accelerated the differentiation of BMSCs into neurons. When the concentration of NGF increased, the absolute values of electrophoretic mobility and zeta potential of the differentiating BMSCs increased. In addition, a longer inductive period yielded higher charge of the differentiating BMSCs and a smaller uptake amount of heparin-functionalized PLGA NPs. However, an increase in the loading efficiency of heparin on PLGA NPs enhanced the uptake and reduced the electrical characteristics of the primary and differentiating BMSCs. Hence, a general rule is drawn that an increase in the uptake of heparin-functionalized PLGA NPs decreased the electrophoretic mobility and zeta potential of BMSCs during differentiation towards neurons.

Nanosuspensions as delivery system for gambogenic acid: characterization and in vitro/in vivo evaluation

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A novel glyceryl monoolein-bearing cubosomes for gambogenic acid: Preparation, cytotoxicity and intracellular uptake

“…The nanoparticles had the higher Zeta potential value, indicating the repulsion force prevents aggregation of the particles and the better stability of this colloidal system (Teeranachaideekul et al, 2007). In this study, Zeta potential was about (À26.60 AE 0.53) mV, testifyng that GNA-Cubs could are stable dispersed owing to electrostatic repulsion between nanoparticles (Mehnert and Mader, 2001;Kuo et al, 2010). Additionally, the steric hindrance from the stabilizer would have an accessional impact on increasing GNA-Cubs stability.…”

Physicochemical properties of nevirapine-loaded solid lipid nanoparticles and nanostructured lipid carriers

“…A high zeta potential represented a stable suspension of the lipid particles. This resulted from the electrostatic repulsion, which could prevent the biocolloids from flocculation [31][32][33][34]. In this study, zeta potential was about 17.5 mV, which was not high as compared with the thermal potential of 25.7 mV.…”