Capillary electrophoresis of natural products: 2011–2012

Abstract

Bioactive natural products are major sources of lead compounds for drug discovery and pharmaceutical development, therefore, innovative and current separation and characterization techniques are important for these compounds. Here, CE methods applied for the analysis of natural products published during 2011-2012 are reviewed. This is an updated version of an earlier review paper in this journal, which highlighted developments during 2006-2010. The major method developments over the review period centered on derivatization, chiral analysis, modes of detection, stacking or on-line sample concentration, and sample preparation (predominantly using extraction methods). The samples analyzed were herbal products, foods, soil, and biological samples. Developments also occurred in the areas of quality control, toxicology assessment, and enzyme-inhibitor screening. A table that summarizes the areas, source of natural product, nature of the bioactive analyte, CE conditions, LODs, and corresponding reference is provided. A short description on the theory of CE and insights on future activities of CE on natural products are also presented.

The relevance of pharmacognosy in pharmacological research on herbal medicinal products

“…Different separation modes are available for the separation of plant constituents: capillary zone electrophoresis (CZE) separates compounds based on the electrophoretic mobilities of the ionic analytes and is, therefore, limited to charged species. In contrast, micellar electrokinetic chromatography (MEKC) allows the separation of both charged and neutral analytes, and it provides additional separation selectivity for charged species that are difficult to separate by CZE, as separation is based on a combination of electrophoretic and chromatographic mechanisms [184]. In recent years, nonaqueous CE (NACE), which is based on electrolyte solutions prepared from pure organic solvents, has become increasingly popular for phytochemical analyses because of the good solubility of many target analytes in the organic mobile phases, alternative selectivity characteristics, reduced electrophoretic currents, and a very good feasibility for coupling with MS detectors [173,185].…”

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

“…The application of CE to the analysis of biotoxins , herbicides , glycerophospho‐ and sphingolipids , antibiotics , amino acids , chiral compounds , biogenic amines , in different food products has been also reviewed. Also, several review papers have been published in the mentioned period focusing on the application of CE to the analysis of natural products and phytochemicals . It is also noteworthy the high number of review papers dealing with the application of CE in metabolomics , including the use of metabolomic approaches to study genetically modified (GM) crops and fermented and functional foods .…”

Cyclodextrin-modified MEKC method for quantification of selected acidic metabolites of catecholamines in the presence of various biogenic amines. Application to diagnosis of neuroblastoma

“…Therefore, there is a growing interest in testing the capacity of CE to BAs analyse in biological matrices [10,[17][18][19][20][21][22][23]. Moreover, CE could be coupled to laser induced fluorescence (LIF), which allows even the nanomolar limits of detection (LODs) to be achieved for most BAs in biological fluids [21][22][23].…”