Capillary electrophoresis of proteins 1999-2001

Abstract

This review article with 223 references describes recent developments in capillary electrophoresis (CE) of proteins and covers papers published during last two years, from the previous review (V. Dolnik, Electrophoresis 1999, 20, 3106-3115) through Spring 2001. It describes the topics related to CE of proteins including modeling of the electrophoretic properties of proteins, sample pretreatment, wall coatings, improving selectivity, detection, special electrophoretic techniques, and applications.

Modification of Resolution in Capillary Electrophoresis for Protein Profiling in Identification of Genetic Modification in Foods

“…The additional problem to solve in separation of proteins is their common adsorption on capillary walls, which significantly deteriorates the efficiency of separation. In order to minimize this behavior various modifications of capillary walls 15,23 or additions of different surface active species to BGE 24 are used. One type of additives to BGE used to suppress adsorption of proteins are different polymers modifying the capillary walls and also altering electrophoretic properties of analytes.…”

“…26,28 Dendrimers can be also a group of materials employed for this purpose. 23 They are also known as unimolecular micelles and are highly symmetric three-dimensional structures, originally referred to as cascade molecules, and composed of initiator core, and repeating units with branching and terminal functional groups. 29 They exhibit numerous interesting properties, such as e.g.…”

Capillary electrophoresis of proteins 2003–2005

“…Previously, we have reviewed the use of CE for the analysis of proteins for the periods of 1992-1997 [1], 1997-1999 [2], 1999-2001 [3], and 2001-2003 [4]. This review particularly emphasizes practical applications of CE, but also includes sections on technique and theory, including modeling migration of proteins and CE systems, sample preparation, coatings, improving separation, detection, and use of various modes of CE.…”

Isoelectric Focusing Sample Injection for Capillary Zone Electrophoresis in a Fused Silica Capillary

“…1,2 However, conventional CE is limited to a relatively poor concentration detection limit due to the low volume loadability of the capillary; 3,4 also, sample preconcentration is always considered before CE separation. [5][6][7][8][9][10][11][12][13][14][15] Among various concentration methods, isoelectric focusing (IEF) has been demonstrated to be an effective approach for the concentration of proteins.…”