Capillary electrophoresis separation of vinpocetine and related compounds: Prediction of electrophoretic mobilities in partly aqueous media

Abstract

Offord's equation, a relationship between electrophoretic mobility and charge, size and shape of peptides, has been extended to quantitate the electrophoretic mobility of vinca alkaloids. Partly aqueous protonation constants and the derived theoretical mobilities have been proven to be able to predict experimental electrophoretic mobilities. In practice, seven vincamine derivatives of very low water-solubility were separated by capillary electrophoresis. Buffer total concentration, apparent pH and methanol content, the three most important parameters of the running buffer, were used in triangular resolution mapping to characterize separation. Even though electrophoresis is well known to slow down in partly aqueous media, under our optimized circumstances a baseline separation was achieved within 8 min in each case.

Produção de metabólitos secundários em cultura de células e tecidos de plantas: o exemplo dos gêneros Tabernaemontana e Aspidosperma

“…A eletroforese capilar é aplicável na determinação de uma grande variedade de amostras, incluindo vitaminas hidro-lipossolúveis, aminoácidos, íons inorgânicos, ácidos orgânicos, fármacos, catecolaminas, substâncias quirais, proteínas, peptídeos e muitos outros (Guttman al., 2004;Mazak et al, 2000). Uma característica que difere a EC das outras técnicas é a sua capacidade única para separar macromoléculas carregadas eletricamente de interesse tanto em indústrias de biotecnologia quanto em pesquisas biológicas.…”

“…Uma característica que difere a EC das outras técnicas é a sua capacidade única para separar macromoléculas carregadas eletricamente de interesse tanto em indústrias de biotecnologia quanto em pesquisas biológicas. Outro aspecto importante é a possibilidade de acoplamento da EC a espectrometria de massas utilizando-se técnicas de ionização suaves como eletrospray, termospray e ionização a pressão atmosférica (Guttman et al, 2004, Mazak et al, 2000.…”

Separation of vinca alkaloid enantiomers by capillary electrophoresis applying cyclodextrin derivatives and characterization of cyclodextrin complexes by nuclear magnetic resonance spectroscopy

“…Capillary electrophoresis is a powerful technique for the analysis of chiral compounds, still, few studies are known concerning the CE analysis of vinca alkaloids [7,8]. In contrast with CE, numerous chromatographic methods have been developed for their analysis [9][10][11].…”

Combinatorial Natural Products: From Cloning to Analysis

“…Both normal and malignant cells exposed to these alkaloids undergo changes characteristic of apoptosis. Vinca rosea cell culture gene expression analysis has been extensively tested using transgenes [9], and various chromatographic [10] and electrophoretic [11] techniques as reported in the literature.…”