Capillary Electrophoresis With a Chemiluminescence Detector Using the Two Reactions of Luminol and Peroxyoxalate

Abstract

& We investigated capillary electrophoresis (CE) with a chemiluminescence (CL) detector using both CL systems of luminol and peroxyoxalate. Flow-and batch-type CL detection cells were used to analyze model samples, such as isoluminol isothiocyanate (ILITC), fluorescein isothiocyanate (FITC), and proteins labeled with these reagents. CE with the flow-type cell allowed analysis of hemoglobin, FITC, and FITC-labeled hemoglobin. Hemoglobin and FITC were detected with the luminol CL system and peroxyoxalate CL system, respectively, in the CE apparatus. On the other hand, FITC-labeled hemoglobin showed elevated CL with both luminol and peroxyoxalate CL systems. The detection limit of FITC-labeled hemoglobin was ca. 0.6 lM. The CE apparatus with batch-type cell was used to analyze ILITC, FITC, and ILITC-, FITC-, and ILITC=FITC-labeled human serum albumin (HSA). ILITC and ILITC-labeled HSA as well as FITC and FITC-labeled HSA were detected with the luminol CL system and with the peroxyoxalate system, respectively. ILITC=FITC-labeled HSA showed elevated CL with both CL systems.

Recent advances in chemiluminescence detection coupled with capillary electrophoresis and microchip capillary electrophoresis

“…The separation and analysis of proteins are important in the area of bioscience. CE–CL system has been applied for hemoprotein, enzyme, and tumor marker protein analysis .…”