Capillary electrophoresis with electrochemiluminescence detection for the simultaneous determination of cisatracurium besylate and its degradation products in pharmaceutical preparations

Abstract

Capillary electrophoresis with electrochemiluminescence detection for the simultaneous analysis of cisatracurium besylate and its degradation products (laudanosine, quaternary monoacrylate) in pharmaceutical preparation was developed and fully validated. The significant parameters that influence capillary electrophoresis separation and electrochemiluminescence detection were optimized. The total analysis time of the analytes was 15 min. The linearities of the method were 0.1∼40.0 μg/mL for cisatracurium besylate and 0.04∼8.00 μg/mL for laudanosine, with correlation coefficients (r) of 0.999 and 0.998, respectively. The detection limits (S/N = 3) were 83.0 ng/mL for cisatracurium besylate and 32.0 ng/mL for laudanosine. The intraday relative standard deviations of the analytes were <3.0%, and the interday relative standard deviations were <8.0%. The developed method was cost-effective, sensitive, fast, and resource-saving, which was suitable for the ingredient analysis in pharmaceutical preparation.

Uniform experimental design in chemometrics

“…Ji, Alaert, and Xu et al proposed sequential UDs to seek the best extracts through the capillary electrophoresis fingerprint of G. biloba . There were other works that used UD to optimize the experimental conditions for good separation to analyze chemical components or to obtain high experimental yields …”

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

“…Other detection schemes are less suitable and much less often utilized for peptide detection in CE and CEC than the above UV‐absorption, fluorescence, and MS detectors. For that reason only some recent reviews are cited here, which are dealing with general developments and applications of these detectors in separation methods, such as contact or contacless capacitively coupled conductivity detection (C 4 D) , electrochemical , chemiluminiscence , electrochemiluminiscence , infrared spectroscopy, and NMR . In these reviews, few applications of these detectors for CE and CEC separations of peptides as model analytes can be found.…”

Cistracurium Besylate 10 mg/mL Solution Compounded in a Hospital Pharmacy to Prevent Drug Shortages: A Stability Study Involving Four Degradation Products

“…Cisatracurium is a non-depolarizing, intermediate-acting, neuromuscular blocker from the benzylisoquinoline family of curares [ 1 , 2 , 3 , 4 , 5 ]. In Europe, cisatracurium is marketed as a besylate salt solution with a concentration ranging from 2 to 5 mg/mL of cisatracurium base [ 1 , 5 , 6 ].…”

“…The drug is used to induce the relaxation of striated muscles during general anesthesia procedures or sedation in the intensive care unit (ICU) [ 1 , 3 , 4 , 5 , 7 , 8 ]. Cisatracurium is the only curare recommended for prolonged infusion in patients with acute respiratory distress syndromes and in the ICU because it is associated with a lower risk of histamine release [ 2 , 8 , 9 ]. Due to the massive number of patients requiring invasive ventilation during the coronavirus disease 2019 (COVID-19) pandemic, the demand for neuromuscular blockers increased dramatically worldwide and led to drug shortages [ 10 , 11 , 12 , 13 , 14 ].…”