Capillary Electrophoresis with Laser-Induced Fluorescence Detection for the Diagnosis of Phenylketonuria: A Versatile Wet or Dry Laboratory Experiment in Upper-Level Undergraduate Analytical Chemistry

Seaton, Kenneth Marshall; Stockton, Amanda M.; O’Mahony, Christy Charlton

doi:10.1021/acs.jchemed.0c01342

Cited by 2 publications

(1 citation statement)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…6 A recent survey also confirms that electrophoresis is prevalent in the biochemistry teaching laboratory. 7 Indeed, electrophoresis experiments are described for upper-level courses in analytical chemistry 8 , 9 and biochemistry 10 laboratories. Efforts to enhance the role of laboratory instruction in chemical education have drawn new attention.…”

Section: Introductionmentioning

confidence: 99%

A Cost-Effective Microfluidic Device to Teach the Principles of Electrophoresis and Electroosmosis

Shaffer,

Herrada,

Walker

et al. 2023

J. Chem. Educ.

View full text Add to dashboard Cite

Electrophoresis is integral to analytical and biochemistry experiences in undergraduate education; however, fundamental principles of the method are often taught in upper-level laboratories through hands-on experiences. A laboratory activity is reported that teaches the concepts of electrophoretic mobility and electroosmotic flow. A single reuseable instrument, called a mini-E, costs 37 USD and consists of a DC power supply, a voltmeter, platinum electrodes, and a chip cast in polydimethylsiloxane. This activity uses common reagents costing only 0.02 USD per student. Experiments are devised that allow students to investigate the properties of electrophoretic flow and electroosmotic flow by separating the two commonly used food dyeing agents Brilliant Blue FCF and Allura Red AC in vinegar and in a solution of ammonium hydroxide. A dark-purple mixture of these dyes is separated into red and blue bands that are easily visualized. The migration order of the dyes differs when the separation is performed under conditions of reversed polarity and suppressed electroosmotic flow (vinegar) compared to conditions of normal polarity and active electroosmotic flow (ammonium hydroxide). When delivered to chemistry majors, students had a significant gain in their ability to apply the concepts of electroosmosis and electrophoresis to predict analyte migration. Although this activity targets upper-level chemistry content, it can also be adapted for other laboratory experiences.

show abstract

Section: Introductionmentioning

confidence: 99%

A Cost-Effective Microfluidic Device to Teach the Principles of Electrophoresis and Electroosmosis

Shaffer,

Herrada,

Walker

et al. 2023

J. Chem. Educ.

View full text Add to dashboard Cite

show abstract

Teaching and learning innovations for distance learning in the digital era: a literature review

Li¹,

Wong²,

Chan³

2023

Front. Educ.

View full text Add to dashboard Cite

This paper presents a review study on teaching and learning innovations (TLIs) for distance learning in higher education, which involves substantial use of technology in its practice. The study covers 247 publications that were published from 2017 to 2022 and collected from Scopus to analyze the patterns and trends of TLIs for distance learning in higher education. The results show four main types of TLIs: educational technologies, teaching and learning approaches and activities, teaching and learning programs, and assessment approaches and activities. They also reveal seven major pedagogical patterns in the TLIs, covering the learning of science through online laboratories, virtual and augmented reality, multimedia, gaming, collaboration, tasks/projects, and blended/hybrid/flipped learning. These findings suggest implications for distance learning, covering the use of online laboratories in science courses, promotion of virtual and augmented-based distance learning, encouragement of development, implementation, and the study of pedagogical approaches to distance learning, as well as the increase in interactivity in multimedia-based distance learning.

show abstract

Capillary Electrophoresis with Laser-Induced Fluorescence Detection for the Diagnosis of Phenylketonuria: A Versatile Wet or Dry Laboratory Experiment in Upper-Level Undergraduate Analytical Chemistry

Cited by 2 publications

References 41 publications

A Cost-Effective Microfluidic Device to Teach the Principles of Electrophoresis and Electroosmosis

A Cost-Effective Microfluidic Device to Teach the Principles of Electrophoresis and Electroosmosis

Teaching and learning innovations for distance learning in the digital era: a literature review

Contact Info

Product

Resources

About