Capillary Electrophoresis with Laser-induced Fluorescence Detection for Application in Intracellular Investigation of Anthracyclines and Multidrug Resistance Proteins

Mbuna, Julius; Kaneta, Takashi

doi:10.2116/analsci.31.1121

Cited by 9 publications

(3 citation statements)

References 72 publications

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“…Out of the many analytical techniques available to study fluorescence, few are as amenable as fluorescence spectroscopy and microscopy. Fluorescence is already adopted by a large portion of electrophoresis users, becuase of LIF detection . Capillary, microchip, and gel electrophoresis all use fluorescence detection as a sensitive means to quantify analyte, often taking advantage of reactive probes or other selective labeling strategies to provide another level of information to the analysis.…”

Section: Introductionmentioning

confidence: 99%

A fluorescence toolbox: A review of investigation of electrophoretic separations, process, and interfaces

Thompson

Pappas

2018

Electrophoresis

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This review focuses on fluorescence spectroscopy techniques for the investigation of electrophoretic separations. Fluorescence has been used as a sensitive detector for capillary, gel, and microchip electrophoresis for decades. However, advanced fluorescence methods can be used to study transport, interfacial phenomena, intermolecular and affinity interactions, and other processes that occur during separation. This so-called spectroscopic toolkit can be implemented to understand fundamental behavior in electrophoresis and electrokinetic chromatography. Techniques such as fluorescence recovery after photobleaching, fluorescence correlation spectroscopy, and fluorescence anisotropy are discussed in relation to electrophoretic separations. Newer methods such as super-resolution microscope are also introduced.

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Section: Introductionmentioning

confidence: 99%

A fluorescence toolbox: A review of investigation of electrophoretic separations, process, and interfaces

Thompson

Pappas

2018

Electrophoresis

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“…15 Takayanagi et al reported on the electrophoretic behavior of graphene, demonstrating that the dispersion of graphene in an aqueous solution can be monitored and that surfactant on the graphene surface is replaced competitively by water-soluble nonionic polymers. 16 CE and MCE can be connected with various detection methods [17][18][19][20] and analysis methods, such as mass spectrometry. This merit affords fruitful information about molecular species in a sample.…”

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confidence: 99%

“…On the other hand, the disadvantage of CE and MCE is low sensitivity. To overcome this problem, highly sensitive detection methods, such as laser-induced fluorescence, 20 preconcentration with a solid-phase extractant, 21 and on-line concentration [22][23][24] have been adopted.…”

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confidence: 99%

Analytical Chemistry by Electrophoresis

Okamoto

2020

ANAL. SCI.

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Laser‐Induced Fluorometry for Capillary Electrophoresis

Kaneta

2018

The Chemical Record

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Laser-induced fluorometry (LIF) has achieved the detection of single molecules, which ranks it among the most sensitive of detection techniques, whereas capillary electrophoresis (CE) is known as a powerful separation method with resolution that is beyond the reach of many other types of chromatography. Therefore, a coupling of LIF with CE has established an unrivaled analytical technique in terms of sensitivity and resolution. CE-LIF has demonstrated excellent performance in bioanalytical chemistry for the high-resolution separation and highly sensitive detection of DNAs, proteins, and small bioactive molecules. This review describes the CE-LIF methods developed by the author's group that include indirect and direct detection using diode lasers, post-column derivatization, and Hadamard transformation, as well as applications to the binding assays of specific DNA immunoassays of proteins and to the determination of anticancer drugs.

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Capillary Electrophoresis with Laser-induced Fluorescence Detection for Application in Intracellular Investigation of Anthracyclines and Multidrug Resistance Proteins

Cited by 9 publications

References 72 publications

A fluorescence toolbox: A review of investigation of electrophoretic separations, process, and interfaces

A fluorescence toolbox: A review of investigation of electrophoretic separations, process, and interfaces

Analytical Chemistry by Electrophoresis

Laser‐Induced Fluorometry for Capillary Electrophoresis

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