Capillary electrophoresis with on-line enrichment for the analysis of biological samples

Sentellas, Sònia; Puignou, L.; Galcerán, M.T.

doi:10.1002/1615-9314(20021101)25:15/17<975::aid-jssc975>3.0.co;2-y

Cited by 54 publications

(29 citation statements)

References 85 publications

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“…15 In order to reduce these deficiencies, two strategies could be used: (i) on-line sample preconcentration methods, which are cheap, and can improve the sensitivity in several orders of magnitude, 16 however these are arduous and diminish the analytical frequency. These disadvantages make this method inappropriate for large volumes; (ii) the use of the laser-induced fluorescence detection system would be another way to improve the detectability and sensitivity of the method.…”

Section: Resultsmentioning

confidence: 99%

Direct determination of plant-growth related metabolites by capillary electrophoresis with spectrophotometric UV detection

Assunção¹,

Arruda²,

Martinelli³

et al. 2009

J. Braz. Chem. Soc.

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A detecção de hormônios e reguladores de crescimento em plantas é de grande interesse em uma enorme variedade de estudos biológicos. Visando este propósito nós desenvolvemos um método simples baseado em eletroforese capilar (CE) para a separação de diferentes classes de reguladores de crescimento de plantas como as auxinas, citocinina, ácidos giberélico e abscíssico. Foi utilizada CE, com detecção na região de UV; as condições de separação foram: fosfato de potássio como tampão, em concentração de 25 mmol L -1 , pH 12 ou 2,5, tempo de injeção 5 s, elevando por sinfonamento o capilar, à altura de 10 cm e voltagem de separação de 22 kV. A absorbância foi fixada a 220 nm ou 270 nm, dependendo da classe do fitohormônio. Nestas condições os fitohormônios (IAA, GA 3 , ABA, picloram, zeatina e BAP) foram separados em um tempo de 3 a 5 min. O material oriundo de planta para verificar a possibilidade de detecção de hormônio/reguladores de crescimento de plantas foi a citro (Citrus sinensis L. Osbeck) callus no estágio de multiplicação. Na amostra de tecido de planta, a zeatina foi detectada com sucesso. Os resultados confirmaram a pontencialidade do uso de CE como um método eficiente, de baixo custo e alternativo para a detecção dessa classe de compostos em tecidos de plantas.The detection of plant hormones and growth regulators is of great interest for many biological studies especially in the determination of metabolites related to plan growth and differentiation. In this work, we propose a simple method based on capillary electrophoresis (CE) for the separation of different classes of plant growth regulators such as auxins, cytokinins, gibberelic acid and abscisic acid. CE with UV detection was used and the analytical conditions were as follows: phosphate buffer 25 mmol L -1 , for all the measurements and the separation conditions pH 12 or 2.5, by hydrodynamic injection 5 s at 10 cm and separation voltage of 22 kV. The absorbance detection was fixed at either 220 nm or 270 nm depending on a given phytohormone class. Under these conditions, phytohormones (Indole-3-acetic acid (IAA), Gibberellic acid (GA 3 ), Abscisic acid (ABA), picloram, zeatin and 6-Benzylaminopurine (BAP) were separated in approximately 3 to 5 min. The plant material used to verify the possibility of detection of hormone/plant growth regulators was citro (Citrus sinensis L. Osbeck) callus in the multiplication stage. In the plant tissue sample, zeatin was successfully detected. The results confirmed the potential use of CE as an efficient alternative and simple method to the classical procedures used for phytohormone detection in plant tissues.Keywords: plant metabolites, capillary electrophoresis, phytohormones, Citrus spp IntroductionThe determination and characterization of metabolites involved in plant growth play an important role in plant morphogenesis studies. Among the substances involved in this process are the plant hormones, which are traditionally classified in the following groups: auxins, cytokinins, gibberellins, abscisic acid, and et...

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Section: Resultsmentioning

confidence: 99%

Direct determination of plant-growth related metabolites by capillary electrophoresis with spectrophotometric UV detection

Assunção¹,

Arruda²,

Martinelli³

et al. 2009

J. Braz. Chem. Soc.

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“…In addition, various off-line and on-line concentration methods as well as electrophoretic stacking procedures have been developed. CE with on-line enrichment for the analysis of biological samples by chromatographic and electrophoretic preconcentration [103] as well as general sample stacking strategies [104,105] have been reviewed recently. Chiral CE in bioanalysis has also been summarized previously [12][13][14][15][16].…”

Section: Enantioseparation Of Drugs In Biological Matricesmentioning

confidence: 99%

Pharmaceutical and biomedical applications of chiral capillary electrophoresis and capillary electrochromatography: An update

Scriba¹

2003

Electrophoresis

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Pharmaceutical and biomedical applications of chiral capillary electrophoresis and capillary electrochromatography: An updateCapillary electrophoresis is often considered an ideal method for the chiral analysis of compounds due to the high separation power of the technique and has therefore found widespread acceptance for the analysis of drugs and pharmaceuticals. In contrast, capillary electrochromatography is still more or less in an infancy state searching for its place among the analytical separation techniques although interesting applications have been published. The present review summarizes recent developments and applications of chiral pharmaceutical analysis by electromigration techniques published in 2002 and early 2003.

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“…To circumvent this drawback, sample preconcentration techniques can be integrated, which can be further classified into offline [3,4] and online [5,6] approaches.…”

Section: Introductionmentioning

confidence: 99%

“…SPE-CE is efficient at concentrating hydrophobic compounds but it has some limitations such as loss of resolution, peak broadening, peak tailing and the increased backpressure that disturbs the electroosmotic flow (EOF) [5]. These limitations can be partially solved by mPC due to the limited volume of solvent required for elution that avoids the adverse effects of the large volumes of organic solvents on CE.…”

Section: Introductionmentioning

confidence: 99%

High‐sensitive protein analysis by FESI‐CE‐MALDI‐MS

2011

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Capillary zone electrophoresis (CZE) and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) are two techniques highly suitable for the separation and detection of intact proteins. Herein, based on the use of a recently introduced iontophoretic fraction collection interface for the coupling of CE and MALDI-MS, the potential of the combination of both techniques for the analysis of intact proteins is assessed. To further provide a bioanalytical platform with high-sensitivity capabilities, field-enhanced sample injection is integrated as on online preconcentration strategy upstream from the electrokinetic separation. Under optimized conditions, more than 3200-and 4800-fold improvement, respectively in terms of peak height and peak area, as well as LODs ranging from 5 to 10 nM, has been achieved.

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Capillary electrophoresis with on-line enrichment for the analysis of biological samples

Cited by 54 publications

References 85 publications

Direct determination of plant-growth related metabolites by capillary electrophoresis with spectrophotometric UV detection

Direct determination of plant-growth related metabolites by capillary electrophoresis with spectrophotometric UV detection

Pharmaceutical and biomedical applications of chiral capillary electrophoresis and capillary electrochromatography: An update

High‐sensitive protein analysis by FESI‐CE‐MALDI‐MS

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