Capillary electrophoretic analysis of proteins and peptides of biomedical and pharmacological interest

Righetti, Pier Giorgio

doi:10.1002/bdd.276

Cited by 58 publications

(42 citation statements)

References 101 publications

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“…A number of CE modes have been developed for the separation and analysis of proteins: isotachophoresis (ITP) [112], isoelectric focusing (IEF) [103,104,106,113], electrokinetic chromatography (EKC) [114][115][116][117][118], affinity electrophoresis [119,120], and immunosubstraction CE (IS-CE) [121]. CE has been used for the diagnosis of such disorders as adenylosuccinase deficiency, 5-oxoprolinuria, Bence-Jones proteinuria, and nephrotic syndrome, and has been gaining importance for use in routine clinical analysis [122][123][124].…”

Section: Proteins Glycans and Glycoproteinsmentioning

confidence: 99%

Recent developments in the determination of urinary cancer biomarkers by capillary electrophoresis

Liu

et al. 2004

Electrophoresis

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Investigation of effective biomarkers for cancers is currently a popular area of study in clinical and cancer researches, because it can potentially lead to pre-cancer screening or pre-cancer diagnosis and may provide useful information on cancer type and the disease's stage of progression. More and more biochemical or chemical fluid components of the human body such as urine, blood, and cerebrospinal fluid have been considered to contain biomarkers, which are useful in cancer researches, pre-cancer diagnosis, and cancer follow-ups during or after cancer treatment. Several modern analytical techniques, such as gas chromatography (GC), high-performance liquid chromatography (HPLC), capillary electrophoresis (CE), and other separation techniques as well as hyphenated techniques, have been extensively used in study of cancer biomarkers. Among these techniques, CE is considered to be a highly efficient and practical analytical technique because of the small sample volume requirement and its wide separation versatility, ranging from small inorganic molecules to large biomolecules. This review discusses the latest developments involving biomarkers and their analysis by CE, including a discussion of instrumental conditions, method developments, and data analysis.

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Section: Proteins Glycans and Glycoproteinsmentioning

confidence: 99%

Recent developments in the determination of urinary cancer biomarkers by capillary electrophoresis

Liu

et al. 2004

Electrophoresis

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“…Capillary zone electrophoresis (CZE) has developed into a fast, high-efficient, and high-sensitive separation technique which is now frequently used for analysis, micropreparation, and physicochemical characterization of peptides [1][2][3][4][5][6][7]. CZE can provide not only the information on qualitative and quantitative composition of peptide samples but also important physicochemical characteristics of separated peptides such as effective and absolute mobilities, charges, and parameters of molecular size and conformation.…”

Section: General Aspectsmentioning

confidence: 99%

Separation and investigation of structure‐mobility relationships of insect oostatic peptides by capillary zone electrophoresis

et al. 2004

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Capillary zone electrophoresis (CZE) has been applied to qualitative analysis, separation, and physicochemical characterization of synthetic insect oostatic peptides (IOPs) and their derivatives and fragments. Series of homologous IOPs were separated in three acidic background electrolytes (BGEs; pH 2.25, 2.30, 2.40) and an alkaline BGE (pH 8.1). Best separation was achieved in acid BGE composed of 100 mM H3PO4, 50 mM Tris, pH 2.25. The effective electrophoretic mobilities, mu(ep), of all IOPs in four BGEs were determined and several semiempirical models correlating effective mobility with charge-to-size ratio (mu(ep) versus q/Mr k) were tested to describe the migration behavior of IOP in CZE. None of models was found to be unambiguously applicable for the whole set of 20 IOPs differing in size (dipeptide - decapeptide) and charge (-2 to +0.77 elementary charges). However, a high coefficient of correlation, 0.9993, was found for the subset of homologous series of IOPs with decreasing number of proline residues at C-terminus, H-Tyr-Asp-Pro-Ala-Prox-OH, x = 6 - 0, for the dependence of mu(ep) on q/Mr k with k = 0.5 for IOPs as anions in alkaline BGE and with k = 2/3 for IOPs as cations in optimized acidic Tris-phosphate BGE. From these dependences the probable structure of IOPs in solution could be predicted.

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“…Moreover, sample pretreatment (such as solid-phase extraction) is often necessary prior to analysis. CE offers several advantages over chromatographic techniques for the analysis of biological samples [6][7][8]. The main advantages of CE over chromatographic methods are higher separation efficiency, higher selectivity, the smaller sample volume required, and minimal sample processing before injection.…”

Section: Introductionmentioning

confidence: 99%

Separation and determination of carnosine-related peptides using capillary electrophoresis with laser-induced fluorescence detection

et al. 2005

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A capillary electrophoresis (CE) method with laser-induced fluorescence (LIF) detection was developed for the separation and detection of carnosine-related peptides (carnosine, anserine, and homocarnosine). A sensitive and fluorogenic regent, 3-(4-carboxybenzoyl) quinoline-2-carboxaldehyde (CBQCA) was selected as a precapillary labeling reagent for imidazole dipeptides to form isoindole derivatives. The optimized molar ratio between CBQCA and peptide was found to be 75:1, and 50 mmol/L borate buffer (pH 9.2) was used for the derivatization in order to achieve good efficiency. Three imidazole dipeptides were baseline-separated within 20 min by using 112 mmol/L sodium borate (pH 10.4-10.8) as running buffer. Concentration detection limits (signal-to-noise ratios) for carnosine, anserine, and homocarnosine were 4.73, 4.37, and 3.94 nmol/L, respectively. This method has been applied to the analysis of human cerebrospinal fluid (CSF) and meat dry powder of pig and sheep. Recoveries were in the range of 82.9-104.8% for homocarnosine in CSF. For carnosine and anserine, the recoveries are 98.3% and 80.2% in meat dry powder of pig and 111.2% and 112.8% in meat dry powder of sheep, respectively.

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Capillary electrophoretic analysis of proteins and peptides of biomedical and pharmacological interest

Cited by 58 publications

References 101 publications

Recent developments in the determination of urinary cancer biomarkers by capillary electrophoresis

Recent developments in the determination of urinary cancer biomarkers by capillary electrophoresis

Separation and investigation of structure‐mobility relationships of insect oostatic peptides by capillary zone electrophoresis

Separation and determination of carnosine-related peptides using capillary electrophoresis with laser-induced fluorescence detection

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