Capillary electrophoretic and mass spectrometric analysis of a polydisperse fluorosurfactant

Abstract

A fluorosurfactant has been studied using capillary electrophoresis and mass spectrometry. The fluorosurfactant, FC134, can be used as a buffer additive in capillary electrophoresis in order to decrease wall adsorption of proteins and in micellar electrokinetic chromatography. However, it has been discovered that this fluorosurfactant is polydisperse, thus containing substances with different lengths and structures. In this work, the fluorosurfactant sample components were separated by capillary electrophoresis. An uncoated as well as a poly(vinyl alcohol)-coated capillary were used with running electrolytes containing methanol and acetic acid. Following the capillary electrophoretic separation, fractions were collected for further analysis by MALDI-MS. Non-fractionated samples were also analyzed both by MALDI-MS and by ESI-MS.

Lack of Association between a Cationic Protein and a Cationic Fluorosurfactant

“…MALDI-MS analysis revealed significant polydispersity in the fluorocarbon part, with n ) 8 as the dominant component and n ) 6 as the major other fraction. 17 All experiments were done in 10 mM sodium phosphate buffer prepared from Na 2 HPO 4 and NaH 2 PO 4 solutions, mixed at the ratio that yielded pH ) 7.0 and pD ) 6.6 in water and heavy water, respectively. The FS solutions were prepared by dilution from a 1000 µM stock solution.…”

Bioanalytical Applications of Capillary Electrophoresis

“…However, an MECC method employing a dynamically coated capillary was used for the separation of the isomerization and hydrolysis product of acyl glucuronides (15). Coated as well as uncoated capillaries were investigated using MECC for the analysis of the polydispersed fluorosurfactant FC134, which can be used in CE runs to reduce wall adsorption of proteins (16). Fractions were collected after the migration of certain components and analyzed with matrix-assisted laser desorption/ionization (MALDI)-MS to determine how this surfactant interacts with different buffer systems and analytes.…”

“…A series of ionene polymers was used as a dynamic capillary coating for analysis of urinary and recombinant human erythropoietin (rhEPO) by CE and CE-ESI-MS (16). Synthetic ionenes are polycationic polymers that strongly absorb to the capillary surface.…”

Progress in capillary electrophoresis coupled to matrix‐assisted laser desorption/ionization – time of flight mass spectrometry

“…However, it was discovered that this fluorosurfactant is polydisperse, thus containing substances with different lengths and structures. Al-Jarah et al [32] developed a CE method with offline MALDI-TOF-MS for the analysis of fluorosurfactant sample components. An uncoated as well as a poly(vinyl alcohol)-coated capillary was used with running electrolytes containing methanol and acetic acid.…”