Capillary Electrophoretic Determination of Resveratrol in Wines

Gu, Xuelin; Creasy, Le; Kester, and April; Zeece, Michael G.

doi:10.1021/jf981211e

Cited by 106 publications

(65 citation statements)

References 25 publications

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“…Resveratrol was well-tolerated and no severe adverse reactions were reported in the two studies. In addition, the present study investigated the doses of resveratrol used in various in vivo and in vitro studies, as well as the concentration required to elicit noteworthy pharmacological activities (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)22,23). In vitro data showed that a minimum of 5 µmol/l resveratrol is required for the chemopreventive effects to be elicited (15).…”

Section: Discussionmentioning

confidence: 99%

“…The trans form is able to undergo isomerization into the cis form upon exposure to ultraviolet irradiation (9). Although the two isomers often exist in combination, the trans form is more biologically active and more frequently investigated (8,9,17,18). Red wine is known to contain a high concentration of trans-resveratrol.…”

Section: Introductionmentioning

confidence: 99%

“…Red wine is known to contain a high concentration of trans-resveratrol. The levels of trans-and cis-resveratrol in 26 wines were evaluated by Gu et al in 1999 (17), and trans-resveratrol levels ranged between 0.987 and 25.4 µmol/l, while cis-resveratrol levels were considerably lower.…”

Section: Introductionmentioning

confidence: 99%

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Bioavailability and safety study of resveratrol 500 mg tablets in healthy male and female volunteers

Sergides

Chirila²,

Silvestro³

et al. 2015

Experimental and Therapeutic Medicine

164

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Abstract.Over the past few decades, trans-resveratrol has received widespread attention as a preventive agent for numerous diseases. Several studies have demonstrated that it has significant biological and pharmacological properties. Trans-resveratrol has been reported to possess anti-oxidant, anti-inflammatory, anticarcinogenic, antidiabetic, anti-aging, cardioprotective and neuroprotective properties, which can be relevant in chronic diseases and longevity in humans. The aim of the present study was to investigate the rate and extend of absorption, and also the safety of resveratrol following a single 500 mg oral dose. This was an open label, single dose, one period, bioavailability study in 15 healthy volunteers under fasting conditions. Blood samples were collected at predefined time points up to 24 h after resveratrol administration, and plasma concentrations of resveratrol and its conjugated (glucuronated and sulphated) metabolites were determined using a validated high performance liquid chromatography/tandem mass spectrometry method. Pharmacokinetic parameters, including C max , AUC 0-t , AUC 0-inf , T max , T 1/2 and MRT, were determined from plasma concentration-time profiles and found to be in good agreement with previously reported data. C max and AUC 0-inf were lower for resveratrol when compared with the values for its glucuronated and sulphated metabolites. C max for resveratrol, glucuronated resveratrol and sulphated resveratrol were 71.2±42.4 ng/ml, 4,083.9±1,704.4 ng/ml and 1,516.0±639.0 ng/ml, respectively, while the AUC 0-inf values were 179.1±79.1 ng/ml, 39,732.4±16,145.6 ng/ml and 14,441.7±7,593.2 ng/ml, respectively. No adverse reactions associated with resveratrol were reported during the study. The plasma concentrations of resveratrol (free and conjugated) were in agreement with those mentioned in the literature, and were adequate to promote the pharmacological activities of resveratrol. In conclusion, resveratrol 500 mg tablets were well-tolerated by all participants of the study.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bioavailability and safety study of resveratrol 500 mg tablets in healthy male and female volunteers

Sergides

Chirila²,

Silvestro³

et al. 2015

Experimental and Therapeutic Medicine

164

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“…A key polyphenol in red wine is resveratrol, trans-3,5,4Ј-trihydroxystilbene, from grape skin. Red wine contains 1-75 mg of transresveratrol/liter (3). Studies in male rats demonstrated that an alcohol-free red wine extract and resveratrol protect the heart from ischemia reperfusion injury (4).…”

mentioning

confidence: 99%

Resveratrol and Estradiol Rapidly Activate MAPK Signaling through Estrogen Receptors α and β in Endothelial Cells

Klinge

Blankenship

Risinger

et al. 2005

Journal of Biological Chemistry

277

204

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Vascular endothelial cells (EC)are an important target of estrogen action through both the classical genomic (i.e. nuclear-initiated) activities of estrogen receptors ␣ and ␤ (ER␣ and ER␤) and the rapid "nongenomic" (i.e. membrane-initiated) activation of ER that stimulates intracellular phosphorylation pathways. We tested the hypothesis that the red wine polyphenol trans-resveratrol activates MAPK signaling via rapid ER activation in bovine aortic EC, human umbilical vein EC, and human microvascular EC. We report that bovine aortic EC, human umbilical vein EC, and human microvascular EC express ER␣ and ER␤. We demonstrate that resveratrol and estradiol (E 2 ) rapidly activated MAPK in a MEK-1, Src, matrix metalloproteinase, and epidermal growth factor receptor-dependent manner. Importantly, resveratrol activated MAPK and endothelial nitric-oxide synthase (eNOS) at nM concentrations (i.e. an order of magnitude less than that required for ER genomic activity) and concentrations possibly achieved transiently in serum following oral red wine consumption. Co-treatment with ER antagonists ICI 182,780 or 4-hydroxytamoxifen blocked resveratrol-or E 2 -induced MAPK and eNOS activation, indicating ER dependence. We demonstrate for the first time that ER␣-and ER␤-selective agonists propylpyrazole triol and diarylpropionitrile, respectively, stimulate MAPK and eNOS activity. A red but not a white wine extract also activated MAPK, and activity was directly correlated with the resveratrol concentration. These data suggest that ER may play a role in the rapid effects of resveratrol in EC and that some of the atheroprotective effects of resveratrol may be mediated through rapid activation of ER signaling in EC.Epidemiological studies have indicated that the consumption of red wine reduces the incidence of mortality from coronary heart disease (CHD) 1 (1, 2). The cardioprotective effect has been attributed to the polyphenol fraction of red wine (1). A key polyphenol in red wine is resveratrol, trans-3,5,4Ј-trihydroxystilbene, from grape skin. Red wine contains 1-75 mg of transresveratrol/liter (3). Studies in male rats demonstrated that an alcohol-free red wine extract and resveratrol protect the heart from ischemia reperfusion injury (4). Rodent studies showed that orally administered resveratrol is absorbed in the gut, has high affinity for heart and liver (5, 6), and is metabolized to glucuronides that have a t1 ⁄2 of ϳ1.5 h (7). A recurrent question is whether resveratrol, at concentrations present in red wine, is effective in vivo. The oral absorption of 25 mg of trans-

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“…These results were later improved by the use of a solid phase extraction step previous to the determination by MEKC [71]. In this case, limits of detection of 0.1 and 0.15 μM for trans and cis resveratrol, respectively, were reached.…”

Section: Resveratrolmentioning

confidence: 99%

Analysis of natural antioxidants by capillary electromigration methods

Herrero

Ibáñez

2005

J of Separation Science

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In this work, an exhaustive survey of capillary electromigration methods used to analyze natural antioxidants is presented together with some discussion of the use of these substances use as functional foods. This review provides an updated and exhaustive overview of the separation and identification by capillary electrophoresis of natural compounds with antioxidant activity found in natural matrices and/or foods. The compounds concerned are catechins, isoflavones, anthocyanins, phenolic acids, vitamins, as well as other less common natural substances that have shown antioxidant activity.

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Capillary Electrophoretic Determination of Resveratrol in Wines

Cited by 106 publications

References 25 publications

Bioavailability and safety study of resveratrol 500 mg tablets in healthy male and female volunteers

Bioavailability and safety study of resveratrol 500 mg tablets in healthy male and female volunteers

Resveratrol and Estradiol Rapidly Activate MAPK Signaling through Estrogen Receptors α and β in Endothelial Cells

Analysis of natural antioxidants by capillary electromigration methods

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