Capillary electrophoretic resolution of the 2′-, 3′- and 5′-isomers of nucleotide monophosphates via the zinc complexes

Cahours, Xavier; Morin, Pascal; Dreux, M.

doi:10.1007/bf02490816

Cited by 6 publications

(8 citation statements)

References 11 publications

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“…Cahours et al [6][7][8] reported the addition of inorganic cations to the buffer could improve the resolution of nucleotide mono-, di-and triphosphates in CZE separation [6][7][8]. In this work, either magnesium chloride or zinc acetate was tested to see whether the resolution of UMP isomer would be improved.…”

Section: Interaction Of Nucleotides With Metal Ionsmentioning

confidence: 89%

“…Cahours et al [8] reported that the phosphate chain of the nucleotide isomers studied has the same apparent negative charge in the weak acidic medium and migration of these nucleosides monophosphate depends upon the nature of the base on the nucleotide. Therefore, the separation of nucleotides is difficult to predict.…”

Section: Effect Of Phmentioning

confidence: 98%

“…The method appears to be very effective to resolve all 12 nucleotide isomers in less than 15 min. Cahours et al [6][7][8] have studied the complexation equilibrium between nucleotides and inorganic cations (Mg 21 , Ca 21 , Cd 21 , and Zn 21 ) by CE. These studies would be useful for optimization of the separation of nucleoside mono-, di-and triphosphates by CE according to their different affinity for inorganic cations added to the buffer.…”

Section: Introductionmentioning

confidence: 99%

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An insight into the phenomena involved in a multiple‐function stationary phase for the capillary electrochromatographic separation of 2'‐, 3'‐, and 5'‐monophosphorylated nucleoside isomers

Lin

Liu

2003

Electrophoresis

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The electrochromatographic separations of 2'-, 3'- and 5'-monophosphates of adenosine, guanosine, cytidine, and uridine were carried out with an open-tubular capillary column which was wall-coated with a highly selective reagent, 28-membered macrocyclic polyamine, 4, 8, 12, 18, 22, 26-hexaaza-1,15-dioxacyclooctaeicosane ([28]ane-N6O2). The effects of pH, composition and concentration of background electrolyte (BGE), applied voltage, column length, and the additive of the BGE, such as metal ions, borate, beta-cyclodextrin and organic solvent on the separation of these monophosphorylated nucleotide isomers were investigated. The results suggested that the interactions between analytes and the bonded groups on the wall predominantly comprise anion coordination and anion exchange in addition to the electrophoresis. A well-resolved electrochromatogram was obtained with the capillary column of 100 cm (75 cm effective length) x 75 microm inside diameter (ID), citrate buffer (20 mM, pH 3.99), applied voltage of -22 kV and detection at 254 nm. Column efficiency was found with the average theoretical plate numbers of 119,500/m and a low detection limit of 0.01 microM level could be achieved for the separation of these isomers.

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Section: Interaction Of Nucleotides With Metal Ionsmentioning

confidence: 89%

Section: Effect Of Phmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

An insight into the phenomena involved in a multiple‐function stationary phase for the capillary electrochromatographic separation of 2'‐, 3'‐, and 5'‐monophosphorylated nucleoside isomers

Lin

Liu

2003

Electrophoresis

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“…So, using negative-end detection mode, AMPs take a longer time to reach the detection window than that in neutrally aqueous BGE. To reduce the migration time of AMP and improve the resolutions of AMPs, boric acid was usually employed, because borate can interact with the vicinal diols of nucleotides to form anionic complexes [34][35][36][37][38] in basic pH condition. In addition, Tris, an organic cation, was usually used as a buffering group in CE [32].…”

Section: Choice Of Buffer System In Nacementioning

confidence: 99%

Development of a NACE method for simultaneous measurement of three adenosine monophosphate isomers in biomimicking prebiotic synthesis without sample pretreatment

et al. 2006

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A practical NACE method was developed for simultaneous determination of three adenosine monophosphate (AMP) isomers. Separation of three AMP isomers was achieved using 200 mM Tris/H(3)BO(3) in acetontrile/water (2:1 v/v) at pH* 10.0 as the running buffer and +25 kV as the applied voltage over a bare fused-silica capillary of 50 microm id x 375 microm od x 54.5 cm (46 cm to the detector window). At 260 nm, the calibration curves were linear in the range of 1-100 microg/mL. The detection limits were less than 0.70 microg/mL. The recovery ranged from 94.5 to 106.4%. The intraday RSDs of the migration times were between 2.1 and 3.0%. The developed NACE method has been successfully applied for the determination of three AMP isomers in the real samples of biomimicking prebiotic synthesis reaction between N-(O,O-diisopropyl) phosphoryl amino acid and adenosine.

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“…Some workers used MEKC to separate nucleotides [19,[23][24][25]. Other approaches derived benefit from the complexation of nucleotides with transient metals for better resolution [26][27][28][29][30].…”

Section: Introductionmentioning

confidence: 98%

Capillary electrophoretic method for nucleotide analysis in cells: Application on inherited metabolic disorders

et al. 2007

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Purine and pyrimidine nucleotides influence many metabolic pathways and their analogs have been widely used in medicine. A capillary electrophoretic method was developed for measuring intracellular nucleotides. The final BGE consisted of 40 mM citric acid with addition of 0.8 mM CTAB titrated by gamma-aminobutyric acid to pH 4.4. The electrophoretic separations were carried out in an uncoated silica capillary (id/od - 75/375 microm; effective/total length - 90/97 cm). The method allows a complete separation of 21 nucleotides and deoxynucleotides within 15 min with separation efficiencies up to 400,000 theoretical plates per meter. Due to the use of an acidic separation medium, the method offers a high selectivity toward the studied analytes versus possible interferences from matrices. Sample preparation was optimized in order to shorten work-time and prevent analyte degradation. The method was applied for analyzing nucleotides in human erythrocytes and Chinese hamster ovary cells. Diagnostic potential for inherited metabolic disorders of nucleotide metabolism is presented.

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Capillary electrophoretic resolution of the 2′-, 3′- and 5′-isomers of nucleotide monophosphates via the zinc complexes

Cited by 6 publications

References 11 publications

An insight into the phenomena involved in a multiple‐function stationary phase for the capillary electrochromatographic separation of 2'‐, 3'‐, and 5'‐monophosphorylated nucleoside isomers

An insight into the phenomena involved in a multiple‐function stationary phase for the capillary electrochromatographic separation of 2'‐, 3'‐, and 5'‐monophosphorylated nucleoside isomers

Development of a NACE method for simultaneous measurement of three adenosine monophosphate isomers in biomimicking prebiotic synthesis without sample pretreatment

Capillary electrophoretic method for nucleotide analysis in cells: Application on inherited metabolic disorders

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