Capillary Electrophoretic Separation of Pteridine Compounds

W., K.; Park, S.I.; Lee, Y.K.; Yim, Joon-Hyuk

doi:10.1515/pteridines.1993.4.4.210

Cited by 8 publications

(6 citation statements)

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“…Each type of tumor shows its own pattern in changes of pteridine concentrations, since different pteridine derivatives may play various roles in different tumor-related diseases [145,146]. Even though CE was used in the determination of pteridines in 1993, the detection limits with UV detection were not good enough for urine sample screening [147]. Han et al [148] have successfully employed CE with laser-induced fluorescence (LIF) detection to determine urinary pterdines (Fig.…”

Section: Some Other Small Biomolecules Cancer Markersmentioning

confidence: 99%

Recent developments in the determination of urinary cancer biomarkers by capillary electrophoresis

Liu

et al. 2004

Electrophoresis

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Investigation of effective biomarkers for cancers is currently a popular area of study in clinical and cancer researches, because it can potentially lead to pre-cancer screening or pre-cancer diagnosis and may provide useful information on cancer type and the disease's stage of progression. More and more biochemical or chemical fluid components of the human body such as urine, blood, and cerebrospinal fluid have been considered to contain biomarkers, which are useful in cancer researches, pre-cancer diagnosis, and cancer follow-ups during or after cancer treatment. Several modern analytical techniques, such as gas chromatography (GC), high-performance liquid chromatography (HPLC), capillary electrophoresis (CE), and other separation techniques as well as hyphenated techniques, have been extensively used in study of cancer biomarkers. Among these techniques, CE is considered to be a highly efficient and practical analytical technique because of the small sample volume requirement and its wide separation versatility, ranging from small inorganic molecules to large biomolecules. This review discusses the latest developments involving biomarkers and their analysis by CE, including a discussion of instrumental conditions, method developments, and data analysis.

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Section: Some Other Small Biomolecules Cancer Markersmentioning

confidence: 99%

Recent developments in the determination of urinary cancer biomarkers by capillary electrophoresis

Liu

et al. 2004

Electrophoresis

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“…3 To overcome the problem of time consumption, high-performance capillary electrophoresis (HPCE) coupled with UV spectrophotometric detection has been used. 4 Recently, a more sensitive detection approach, i.e. laser induced fluorescence detection, was used in the HPCE detection of pterines, and a high detection sensitivity was obtained.…”

Section: Introductionmentioning

confidence: 99%

Surface‐enhanced Raman scattering of xanthopterin adsorbed on colloidal silver

Feng

Liang

et al. 2001

J Raman Spectroscopy

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Surface-enhanced Raman scattering (SERS) of xanthopterin adsorbed on colloidal silver was measured and the Raman spectrum calculated by the density functional theory method was also obtained. Xanthopterin can be detected down to 5 × 10 −9 M and the enhancement of the scattering intensity is at least 10 5 -fold. Xanthopterin molecules are adsorbed flatly on the surface of the Ag particles. This study shows that SERS could be another prospective method for the detection of pterines.

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“…HPCE is a very good alternative for HPLC due to its high efficiency, high speed, and small sample size requirements. Cha and coauthors 13 used HPCE coupled with UV spectrophotometric detection as an alternative to separate different kinds of pteridines. But their work only focused on standard samples that were not of physiological significance.…”

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confidence: 99%

Pteridine Analysis in Urine by Capillary Electrophoresis Using Laser-Induced Fluorescence Detection

et al. 1999

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Pteridines are a class of compounds excreted in urine, the levels of which are found to elevate significantly in tumor-related diseases. For the first time, we have developed a method, based on high-performance capillary electrophoresis (HPCE) and laser-induced fluorescence (LIF) detection, to monitor the pteridine levels in urine. HPCE provides better separation than high-performance liquid chromatography and the LIF detector enables us to detect minute amounts of pteridines in body fluid. Eight different pteridine derivatives were well separated in 0.1 M Tris-0.1 M borate-2 mM EDTA buffer (pH 8.75) using a 60-cm fused-silica capillary (50-micron i.d., 35-cm effective length), six of which were detected and characterized in urine samples from normal persons and different cancer patients. The detection limits of these pteridines are under 1 x 10(-10) M. The levels of neopterin, pterine, xanthopterin, and pterin-6-carboxylic acid were found to be significantly elevated in urine excreted by cancer patents, while the level of isoxanthopterin dropped in these patients. No significant change of biopterin level was found between healthy individuals and cancer patients. This method can be used in clinical laboratories either for cancer monitoring or for precancer screening.

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Capillary Electrophoretic Separation of Pteridine Compounds

Cited by 8 publications

References 0 publications

Recent developments in the determination of urinary cancer biomarkers by capillary electrophoresis

Recent developments in the determination of urinary cancer biomarkers by capillary electrophoresis

Surface‐enhanced Raman scattering of xanthopterin adsorbed on colloidal silver

Pteridine Analysis in Urine by Capillary Electrophoresis Using Laser-Induced Fluorescence Detection

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