Capillary electrophoretic study of the complexation of nucleotides with magnesium and calcium ions

Cahours, Xavier; Morin, Pascal; Dreux, M.

doi:10.1007/bf02467641

Cited by 18 publications

(21 citation statements)

References 32 publications

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“…Cahours et al [6][7][8] reported the addition of inorganic cations to the buffer could improve the resolution of nucleotide mono-, di-and triphosphates in CZE separation [6][7][8]. In this work, either magnesium chloride or zinc acetate was tested to see whether the resolution of UMP isomer would be improved.…”

Section: Interaction Of Nucleotides With Metal Ionsmentioning

confidence: 94%

“…The method appears to be very effective to resolve all 12 nucleotide isomers in less than 15 min. Cahours et al [6][7][8] have studied the complexation equilibrium between nucleotides and inorganic cations (Mg 21 , Ca 21 , Cd 21 , and Zn 21 ) by CE. These studies would be useful for optimization of the separation of nucleoside mono-, di-and triphosphates by CE according to their different affinity for inorganic cations added to the buffer.…”

Section: Introductionmentioning

confidence: 99%

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An insight into the phenomena involved in a multiple‐function stationary phase for the capillary electrochromatographic separation of 2'‐, 3'‐, and 5'‐monophosphorylated nucleoside isomers

Lin

Liu

2003

Electrophoresis

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The electrochromatographic separations of 2'-, 3'- and 5'-monophosphates of adenosine, guanosine, cytidine, and uridine were carried out with an open-tubular capillary column which was wall-coated with a highly selective reagent, 28-membered macrocyclic polyamine, 4, 8, 12, 18, 22, 26-hexaaza-1,15-dioxacyclooctaeicosane ([28]ane-N6O2). The effects of pH, composition and concentration of background electrolyte (BGE), applied voltage, column length, and the additive of the BGE, such as metal ions, borate, beta-cyclodextrin and organic solvent on the separation of these monophosphorylated nucleotide isomers were investigated. The results suggested that the interactions between analytes and the bonded groups on the wall predominantly comprise anion coordination and anion exchange in addition to the electrophoresis. A well-resolved electrochromatogram was obtained with the capillary column of 100 cm (75 cm effective length) x 75 microm inside diameter (ID), citrate buffer (20 mM, pH 3.99), applied voltage of -22 kV and detection at 254 nm. Column efficiency was found with the average theoretical plate numbers of 119,500/m and a low detection limit of 0.01 microM level could be achieved for the separation of these isomers.

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Section: Interaction Of Nucleotides With Metal Ionsmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

An insight into the phenomena involved in a multiple‐function stationary phase for the capillary electrochromatographic separation of 2'‐, 3'‐, and 5'‐monophosphorylated nucleoside isomers

Lin

Liu

2003

Electrophoresis

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“…Indeed, it has been reported several times in the literature [25][26][27][28][29] as Mg 2+, Ca 2+, Zn 2+, Mn 2+, Cd2+...) and nucleotides or nucleic acids and reported an affinity scale of metal ions versus phosphate groups and bases of nucleotides.…”

Section: Addition Of a Complexing To The Buffer (Cadmium Ion)mentioning

confidence: 99%

Separation of nucleoside mono-, di- and triphosphates by capillary electrophoresis via cadmium complexation

1999

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SummaryThe CE separation of twelve nucleotides (5'-mono-, di-, triphosphates of adenosine, guanosine, cytidine and uridine) was improved by adding cadmium ion to the ammonium citrate/citric acid buffer (pH 5, ionic strength 100 mM). Cadmium ion acts as a complexing agent for some nucleotides (ATP, CTP, GTP, UTP, GDP). In order to accelerate the separation, the electroosmotic flow was reversed by flushing the fused-silica capillary with 0.2 % aqueous solution of the polycationic surfactant hexadimethrine bromide. A good separation of the twelve nucleotides studied was then achieved on a dynamically coated capillary in less than 5 min by using an ammonium citrate/citric acid buffer (pH 5, ionic strength 100 mM) to which 2 mM cadmium ion has been added. High peak efficiencies were obtained (210 000 theoretical plates) and the resolution between two adjacent peaks was always greater than 1.5.

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“…Some workers used MEKC to separate nucleotides [19,[23][24][25]. Other approaches derived benefit from the complexation of nucleotides with transient metals for better resolution [26][27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoretic method for nucleotide analysis in cells: Application on inherited metabolic disorders

et al. 2007

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Purine and pyrimidine nucleotides influence many metabolic pathways and their analogs have been widely used in medicine. A capillary electrophoretic method was developed for measuring intracellular nucleotides. The final BGE consisted of 40 mM citric acid with addition of 0.8 mM CTAB titrated by gamma-aminobutyric acid to pH 4.4. The electrophoretic separations were carried out in an uncoated silica capillary (id/od - 75/375 microm; effective/total length - 90/97 cm). The method allows a complete separation of 21 nucleotides and deoxynucleotides within 15 min with separation efficiencies up to 400,000 theoretical plates per meter. Due to the use of an acidic separation medium, the method offers a high selectivity toward the studied analytes versus possible interferences from matrices. Sample preparation was optimized in order to shorten work-time and prevent analyte degradation. The method was applied for analyzing nucleotides in human erythrocytes and Chinese hamster ovary cells. Diagnostic potential for inherited metabolic disorders of nucleotide metabolism is presented.

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Capillary electrophoretic study of the complexation of nucleotides with magnesium and calcium ions

Cited by 18 publications

References 32 publications

An insight into the phenomena involved in a multiple‐function stationary phase for the capillary electrochromatographic separation of 2'‐, 3'‐, and 5'‐monophosphorylated nucleoside isomers

An insight into the phenomena involved in a multiple‐function stationary phase for the capillary electrochromatographic separation of 2'‐, 3'‐, and 5'‐monophosphorylated nucleoside isomers

Separation of nucleoside mono-, di- and triphosphates by capillary electrophoresis via cadmium complexation

Capillary electrophoretic method for nucleotide analysis in cells: Application on inherited metabolic disorders

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