1993
DOI: 10.1021/ac00071a025
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Capillary electroseparations of some di-, tri-, and tetrapeptides and enkephalin-related peptides

Abstract: Electrophoretic mobilities and capacity factors for short peptides were determined in micellar electrokinetic systems (MEKC) using UV detection at 210 nm. On the basis of determined capacity ratios, taurodeoxycholic acid was found to be a selective micellar agent for peptides. Structural features other than hydrophobicity were found to be decisive for the distribution of the peptides to the micellar phase. Basic peptides of enkephalin-type containing arginine were more highly distributed to the micelles than o… Show more

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Cited by 21 publications
(9 citation statements)
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“…18,[74][75][76][77][78][79][80][81] expected to give the best shield of silanol groups present on bare silica. Usually a neutral, hydrophilic polymer is attached to the wall through siloxane bonds.…”
Section: Capillary Zone Electrophoresis Of Proteins and Peptidesmentioning
confidence: 99%
See 1 more Smart Citation
“…18,[74][75][76][77][78][79][80][81] expected to give the best shield of silanol groups present on bare silica. Usually a neutral, hydrophilic polymer is attached to the wall through siloxane bonds.…”
Section: Capillary Zone Electrophoresis Of Proteins and Peptidesmentioning
confidence: 99%
“…While the method was originally invented to separate neutral species, it can also be applied to charged molecules, such as small peptides. Both electrostatic and hydrophobic forces are decisive for the distribution of the peptides to the micellar phase [76]. Various detergents (with different hydrophobicity or charge) and/or organic modifiers (e.g., acetonitrile, methanol, etc.)…”
Section: Micellar Electrokinetic Chromatography Of Proteins and Peptidesmentioning
confidence: 99%
“…Seven enkephalin-related peptides containing three to six amino acids were completely separated by CZE using phosphate as the running buffer (pH 3.1) in an amino-silylated fused capillary [15], and the mixtures of the same analytes have been also completely separated by MEKC [14].…”
Section: Introductionmentioning
confidence: 99%
“…Thus, separation of closely related peptides would be difficult under this condition. Incorporation of pseudophases such as micelles and cyclodextrins (CDs) would provide additional selectivity through introduction of hydrophobic interaction or molecular inclusion complexation (especially for separation of enantiomers) [5][6][7][8][9][10][11][12]. Other works have reported the use of single and double chain surfactants for dynamic coating of capillaries [13,14] and covalent coating with aminopropylsilane [15].…”
Section: Introductionmentioning
confidence: 99%