Capillary gas chromatography of the terpenic fraction of Juniperus communis L. Black, Green, Berry, and Leaf Extracts

Abstract

A.U. 5c C I I I I 350 450 550 650 (nrnl Figure 2 PA spectra of pure picramidopropyl silica (sample 11) and the same sorbent with adsorbed anthracene.sample (I). One can conclude that Sample I contains species absorbing in the 450-750 nm region and that the reaction of NH2silica with picryl chloride is less selective than the other procedures.The increased absorption around 500 nm may be caused by a charge-transfer band arrising, e.g., from interactions of unreacted NH2-groups with bonded picramide ligands; a red complex formed from N-propyl picramide and n-propylamine was studied by us in dichloromethane. This interaction influences separation in charge-transfer HPLC of polycondensed aromatic hydrocarbons [4]. Also anthracene adsorbed on picramidopropyl silica causes an increase of absorption near 480 nm as shown in Figure 2. The slight color change of the original Sample II after adsorption of anthracene is in accordance with relative weakness of the chargetransfer interaction observed during chromatographic separations of polycondensed aromatic hydrocarbons on picramidopropyl silica [4].

Chemical Investigation of the Leaf Oil ofJuniperus communisL.

“…Oven temp: 45"-240°C (3"C/min); carrier gas: helium (pressure 200 Kpa); splitting ratio 1:40; scan time, 1 sec.RESULTS AND DISCUSSION:The distillation experiments were performed by the European Pharinacopoeia apparatus. The average yield of the oil was 0.85% v/w, approximately midpoint between the previous yield values (0.3-1.7%) reported in literature(2,4,(5)(6)(7).…”

“…It is our belief that this new direction for the research was to either increase our knowledge on the chemical composition of the leaf oils of various species (2)(3)(4)(5), or use the oils as genetic markers to better understand taxonomic interrelationships (6)(7)(8).…”

Investigation on the Supercritical CO2Extraction of the Volatile Constituents fromJuniperus communisObtained under Different Treatments of the ”Berries” (Cones)

“…The extraction runs were executed under combination of the above conditions. The CO 2 extractions were carried out for 1 hour, and the density of CO 2 used was in the vicinity of 0.290 g/ cm 3 , deemed to be most favorable from preliminary studies.…”

“…In the last few years a number of papers dealt with the composition of the volatile oil from Juniperus communis ªberriesº (cones) [2], [3], [4], [5], [6]. All studies involved the analysis of the com-position of the distilled oil.…”