Capillary Gel Electrophoresis of Proteins: Historical overview and recent advances

Hajba, László; Jeong, Sunkyung; Chung, Doo Soo; Guttman, András

doi:10.1016/j.trac.2023.117024

Cited by 17 publications

(8 citation statements)

References 89 publications

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“…That is, the SDS‐PAGE profiles provided information on the composition and molecular weight distribution of these proteins, which can be attributed to a lower amount of allergen present. This means that above 5 mJ/cm 2 , it will be more likely to decrease in protein bands caused by hydrolysis, producing fragments and aggregates in the SDS‐PAGE wells (Alhendi, 2020; Hajba et al, 2023; Tammineedi et al, 2013). Furthermore, the disappearance after reduction of these proteins (Figure 3a,c,d) can be attributed to aggregates of disulfide bonds (Jiang et al, 2023).…”

Section: Resultsmentioning

confidence: 99%

“…On the other hand, Yang et al (2010) reported that PUV generated a decrease in soy allergen levels (glycinin and conglycinin), which was analyzed by SDS‐PAGE. This method has been applied in a variety of industries for the analysis of viral vaccine proteins, monoclonal antibodies, allergens of all kinds, molecular mass evaluation, and recombinant protein purity evaluation, among others (Hajba et al, 2023). Overall, the SDS‐PAGE profiles of the protein samples treated with UV irradiation revealed changes in protein composition, immunoreactivity, and molecular weight distribution, highlighting the impact of UV light on the protein characteristics of wheat gliadin in the flour samples (Alhendi & Alobaidy, 2023).…”

Section: Resultsmentioning

confidence: 99%

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Effect of pulsed ultraviolet light on the degree of antigenicity and hydrolysis in cow's milk proteins

Salazar,

Pizarro‐Oteíza,

Kasahara

et al. 2024

J Food Process Engineering

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In order to reduce the quantity of allergens that are causing harm to consumer, it is imperative that the proteins found in cow's milk undergo controlled hydrolysis. Investigated were the effects of different pulsed UV light fluences (0.1–10 J/cm2) on samples of commercial cow's milk proteins (CCMP: casein (CN), α‐lactalbumin (α‐LA), and β‐lactoglobulin (β‐LG)) and reconstituted cow's milk proteins (RCMP). The degree of hydrolysis (DH) and sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) profiles in RCMP and CCMP, as well as the degree of antigenicity in RCMP, were assessed for this purpose. The DH increased in CCMP and RCMP samples at the highest fluence applied. SDS PAGE profiles showed a decrease in the intensity of the bands of the CCMP samples for fluences of 5 and 10 J/cm2; however, this was not the case for α‐LA. Finally, the degrees of antigenicity measured with Elisa assay in CN and β‐LG were reduced by 24% and 47%, respectively, at a fluence of 10 J/cm2. Therefore, this nonthermal technology could be an alternative to conventional treatments to reduce the allergenic potential of cow's milk proteins; however, future clinical trials to confirm an anti‐allergenic effect on consumer are needed.Practical ApplicationsPulsed UV light is a nonthermal technology of great relevance in the dairy industry. The aim of this article was to generate knowledge of the effect of pulsed UV light on allergenic dairy proteins. Therefore, this article aimed to demonstrate that pulsed UV light can be a novelty compared with conventional methods of inactivation of commercial and reconstituted dairy allergenic proteins.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Effect of pulsed ultraviolet light on the degree of antigenicity and hydrolysis in cow's milk proteins

Salazar,

Pizarro‐Oteíza,

Kasahara

et al. 2024

J Food Process Engineering

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“…Electrophoresis is one of the most important and widely used techniques in biochemistry and molecular biology [1][2][3][4]. For example, isoelectric focusing (IEF) was applied in high-resolution protein separation [5,6], array capillary electrophoresis (CE) was the key tool for high throughput DNA and RNA sequencing [7], and gel electrophoresis was commonly used in protein research [8].…”

Section: Introductionmentioning

confidence: 99%

A facile double moving redox boundary model for visual electrophoresis titration of ascorbic acid

Liu,

Chen,

et al. 2024

Electrophoresis

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In this work, we proposed a double moving redox boundary (MROB) model to realize the colorless analyte electrophoresis titration (ET) by the two steps of the redox reaction. Single MROB has been proposed for the development of ET sensing (Analyst, 2013, 138, 1137. ACS Sensor, 2019, 4, 126.), and faces great challenges in detecting the analyte without color change during redox reaction. Herein, a novel model of double‐MROB electrophoresis, including its mechanisms, equations, and procedures, was developed for titration by using ascorbic acid as a model analyte. The first MROB was created with ferric iron (Fe3+) and iodide ion (I−) in which Fe3+ was reduced as Fe2+ and I− was oxidized as molecular iodine (I2) used as an indicator of visible MROB due to blue starch–iodine complex. The second boundary was then formed between the molecular iodine and model analyte of ascorbic acid. Under given conditions, there was a quantitative relationship between velocity of MROB (VMROB(ii)) and ascorbic acid concentration (CVit C) in the double‐MROB system (1/VMROB(ii) = 0.6502CVit C + 4.5165, and R = 0.9939). The relevant relative standard deviation values of intraday and inter‐day were less than ∼5.55% and ∼6.64%, respectively. Finally, the titration of ascorbic acid in chewable vitamin C tablets was performed by the developed method, the titration results agreed with those via the classic iodometric titration. All the results briefly demonstrated the validity of the double MROB model, in which Vit C was used as a model analyte. The developed method had potential use in quantitative analysis of redox‐active species in biomedical samples.

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“…Over the past decade, high-performance liquid chromatography and capillary electrophoresis have been routinely used to analyze therapeutic proteins. Sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) in combination with UV or laser-induced fluorescence (LIF) detection is a frequently applied method for rapid protein characterization with high sensitivity . While the innate fluorescence of several amino acid building blocks like tyrosine and tryptophan can be utilized in fluorescence detection, the achievable detection limit is not always satisfactory .…”

Section: Introductionmentioning

confidence: 99%

In Migratio Noncovalent Fluorophore Labeling of Proteins by Propidium Iodide in Sodium Dodecyl Sulfate Capillary Gel Electrophoresis

Auer,

Guttman

2024

Anal. Chem.

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Capillary Gel Electrophoresis of Proteins: Historical overview and recent advances

Cited by 17 publications

References 89 publications

Effect of pulsed ultraviolet light on the degree of antigenicity and hydrolysis in cow's milk proteins

Effect of pulsed ultraviolet light on the degree of antigenicity and hydrolysis in cow's milk proteins

A facile double moving redox boundary model for visual electrophoresis titration of ascorbic acid

In Migratio Noncovalent Fluorophore Labeling of Proteins by Propidium Iodide in Sodium Dodecyl Sulfate Capillary Gel Electrophoresis

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