Capillary gel electrophoresis of very high molecular weight glycoproteins. Commercial and tailor-made gels for analysis of human monomeric and secretory immunoglobulin A

Puerta, Angel de la; Garcia-Lopez, Daniel; Tejedor-Matellanes, Paula; Gomez-Ruiz, Laura; Frutos, Mercedes de

doi:10.1016/j.chroma.2022.463689

Cited by 3 publications

(1 citation statement)

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“…However, CE emerged as a mature and robust separation technique increasingly popular in peptidomic and proteomic analysis [13][14][15][16][17][18][19]. CE methods can be found in all major pharmacopeias and are successfully utilized in biopharmaceutical research and industry [20][21][22][23][24][25][26][27]. CE, as an electric fieldmediated separation technique first introduced in 1981 [28], offers different selectivity and therefore complementarity to LC techniques.…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis‐mass spectrometry for intact protein analysis: Pharmaceutical and biomedical applications (2018–March 2023)

Maráková

Opetová

Tomašovský

2023

J of Separation Science

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Capillary electrophoresis is recognized as a valued separation technique for its high separation efficiency, low sample consumption, good economic and ecological aspects, reproducibility, and complementarity to traditional liquid chromatography techniques. Capillary electrophoresis experiments are generally performed utilizing optical detection, such as ultraviolet or fluorescence detectors. However, in order to provide structural information, capillary electrophoresis hyphenated to highly sensitive and selective mass spectrometry has been developed to overcome the limitations of optical detections. Capillary electrophoresis‐mass spectrometry is increasingly popular in protein analysis, including biopharmaceutical and biomedical research. It is frequently applied for the determination of physicochemical and biochemical parameters of proteins, offers excellent performance for in‐depth characterizations of biopharmaceuticals at various levels of analysis, and has been also already proven as a promising tool in biomarker discovery. In this review, we focus on the possibilities and limitations of capillary electrophoresis‐mass spectrometry for protein analysis at their intact level. Various capillary electrophoresis modes and capillary electrophoresis‐mass spectrometry interfaces, as well as approaches to prevent protein adsorption and to enhance sample loading capacity, are discussed and the recent (2018–March 2023) developments and applications in the field of biopharmaceutical and biomedical analysis are summarized.

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