Capillary ion electrophoresis of inorganic anions and uric acid in human saliva using a polyvinyl alcohol coated capillary column and hexamethonium chloride as additive of background electrolyte

Mori, Miwako; Yamamoto, Tsukasa; Kaseda, Maki; Yamada, Sachiko; Itabashi, Hideyuki

doi:10.1016/j.jchromb.2011.12.027

Cited by 17 publications

(10 citation statements)

References 33 publications

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“…The LOD can be further improved if on‐line CE‐MS technique is applied . Recently, Mori et al reported that LOD for uric acid separated using silane‐coupled PVA covalent coatings is 3.42–6.87 μM, which is much higher than ours.…”

Section: Resultscontrasting

confidence: 49%

Novel covalently coated diazoresin/polyvinyl alcohol capillary column for the analysis of proteins by capillary electrophoresis

Liu

Cong

et al. 2012

Electrophoresis

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A novel method for the preparation of covalently linked capillary coatings of PVA was demonstrated using photosensitive diazoresin (DR) as coupling agents. Layer-by-layer self-assembly film of DR and PVA based on hydrogen bonding was first fabricated on the inner wall of capillary, then the hydrogen bonding was converted into covalent bonding after treatment with UV light through the unique photochemistry reaction of DR. The covalently bonded coatings suppressed basic protein adsorption on the inner surface of capillary, and thus a baseline separation of lysozyme, cytochrome c and BSA was achieved using CE. Compared with bare capillary or noncovalently bonded DR/PVA coatings, the covalently linked DR/PVA capillary coatings not only improved the CE separation performance for proteins, but also exhibited good stability and repeatability. Due to the replacement of highly toxic and moisture-sensitive silane coupling agent by DR in the covalent coating preparation, this method may provide a green and easy way to make the covalently coated capillaries for CE.

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Section: Resultscontrasting

confidence: 49%

Novel covalently coated diazoresin/polyvinyl alcohol capillary column for the analysis of proteins by capillary electrophoresis

Liu

Cong

et al. 2012

Electrophoresis

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“…Centrifugation was carried out at the acceleration (Relative Centrifugal Force) of 8610 g. 3. The capillary electrophoresis technique is widely described in the literature (Demkowska, Polkowska, & Namieśnik, 2008;Guo et al, 2016;Mori et al, 2016;Mori, Yamamoto, Kaseda, Yamada S, & Itabashi, 2012); this article provides an indepth description of conditions for analysis and preparation of the samples (Bel'skaya, 2017). With regard to the pH measurement, saliva was collected into test tubes with tightly screwing caps, and pH was measured immediately after the opening of the tubes.…”

mentioning

confidence: 99%

Diurnal rhythm and salivary electrolyte

Proctor

2018

Archives of Oral Biology

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“…[6][7][8][9][10][11][12] They are involved in several physiological functions, and, depending on the concentration, they can also be associated with adverse effects. [6][7][8][9][10][11][12] Among them, thyroid gland diseases still remain a common global problem and affect around 4% of the world's population. 10,12 Other physiological problems such as hematologic and hormonal diseases, growth retardation, and insomnia can also be mentioned.…”

Section: Introductionmentioning

confidence: 99%

“…[6][7][8][9][10][11][12] Even now, the action mechanisms of bromine and iodine in humans are not well elucidated, and the scientific community related to this demand is currently mobilized. [6][7][8][9][10][11][12] The mobility assessment of bromine and iodine in humans through the analysis of biological samples may help to clarify some doubts related to their respective metabolic routes. Biological samples obtained by non-invasive collections such as hair, saliva, urine, and nail are being slowly proposed as biomarkers by the scientific community.…”

Section: Introductionmentioning

confidence: 99%

Feasibility of microwave‐induced combustion combined with inductively coupled plasma mass spectrometry for bromine and iodine determination in human nail

Novo

Henn

Flores

et al. 2020

Rapid Comm Mass Spectrometry

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Rationale Bromine and iodine have important physiological functions; however, in inadequate concentration, they can also cause several physiological problems. Their mobility assessment in human organisms through biological sampling may help clarify some doubts related to metabolic routes, which are still not well elucidated. In this context, a suitable analytical method for this purpose should be developed. Methods An analytical method for determining ultratrace levels of bromine and iodine in human nail samples was developed. Inductively coupled plasma mass spectrometry (ICP‐MS) using a conventional nebulization system was immediately chosen as the determination tool because of its powerful sensitivity and selectivity. Sample preparation methods including microwave‐induced combustion (MIC), microwave‐assisted extraction, and microwave‐assisted digestion were evaluated. The compatibility of the final solutions with ICP‐MS analysis was considered while the method was developed. Results MIC was chosen as the most suitable method for the sample preparation for determining the levels of bromine and iodine in human nail samples using ICP‐MS. Unlike other sample preparation methods, this one fully eliminated interferences related to the carbon content and memory effects. Sample masses up to 100 mg were efficiently digested, and the analytes were quantitatively absorbed using only 50 mmol L−1 NH4OH solution. Recoveries ranged from 93% to 102%, and the relative standard deviation was < 8%. Conclusions The proposed analytical method presents important characteristics for routine analysis. It allows ultratrace determination even when low sample masses are used because of the low blank values, reduced volume of reagents, and powerful detectability using ICP‐MS.

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Capillary ion electrophoresis of inorganic anions and uric acid in human saliva using a polyvinyl alcohol coated capillary column and hexamethonium chloride as additive of background electrolyte

Cited by 17 publications

References 33 publications

Novel covalently coated diazoresin/polyvinyl alcohol capillary column for the analysis of proteins by capillary electrophoresis

Novel covalently coated diazoresin/polyvinyl alcohol capillary column for the analysis of proteins by capillary electrophoresis

Diurnal rhythm and salivary electrolyte

Feasibility of microwave‐induced combustion combined with inductively coupled plasma mass spectrometry for bromine and iodine determination in human nail

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