Capillary isoelectric focusing

Abstract

Capillary isoelectric focusing (CIEF) provides excellent resolution of proteins with the advantage that separations are carried out in a capillary format with on-tube detection and automated analysis. Recent advances in the technique provide improved resolution, reproducibility and reliability. This review summarizes improvements in sample preparation, capillary selection, and focusing and zone mobilization procedures which have helped CIEF become a more robust analytical method. New applications are reviewed, in particular the use of CIEF in analysis of biopharmaceutical products.

Fatty Acyl Structures of Mycobacterium tuberculosis Sulfoglycolipid Govern T Cell Response

“…The mobilization step was completed after 30 min at 21 kV by applying 0.7 psi pressure at the anode. During separation, the capillary temperature was maintained at 20°C, and CD1b and CD1b:lipid complexes were detected at 280 nm (24,25).…”

Recent developments in the determination of urinary cancer biomarkers by capillary electrophoresis

“…CE is the most promising analytical technique for protein investigations due to its unique advantages over the conventional protein separation techniques like GE and HPLC [28,[103][104][105][106][107][108][109][110][111]. A number of CE modes have been developed for the separation and analysis of proteins: isotachophoresis (ITP) [112], isoelectric focusing (IEF) [103,104,106,113], electrokinetic chromatography (EKC) [114][115][116][117][118], affinity electrophoresis [119,120], and immunosubstraction CE (IS-CE) [121].…”

Simultaneous analysis of endogenous neurotransmitters and neuropeptides in brain tissue using capillary electrophoresis — microelectrospray-tandem mass spectrometry

“…High sensitivity is crucial when analyzing endogenous compounds since the expected concentrations are low; for instance, leucineenkephalin in brain extracts is present in the 18±900 pmol/g (10±500 ng/g) level [6]. Several attempts have been made to use different preconcentration techniques combined with CE and CE-MS, using membranes (mPC) or solid phases [7±10], or electrophoretic techniques such as stacking [11±13], isoelectric focusing (CIEF) [14,15] or isotachophoresis (ITP) [16±18]. The purpose of these techniques is to increase the injection volumes in CE to achieve better sensitivity.…”