Capillary Isotachophoresis Determination of Trace Oxidized Glutathione in Blood

Bodor, Róbert; Nečasová, Andrea; Pechová, Alena; Masár, Marián

doi:10.1515/hjic-2018-0004

Cited by 5 publications

(3 citation statements)

References 17 publications

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“…This may be the cause of the higher values in comparison to ours. Bodor et al (2018) used two different methods for the evaluation of glutathione in the whole blood of dairy cows. The enzymatic spectrophotometric method and isotachophoresis were applied to compare the concentrations of GSH and GSSG.…”

Section: Discussionmentioning

confidence: 99%

“…Mostly, spectrophotometric-and high-performance liquid chromatography (HPLC) based methods are used. Isotachophoresis (ITP) has proven to be a quick and cheap method for the detection of both GSH and GSSG (Bodor et al 2018). No standard method exists nowadays, therefore, comparing the results was difficult due to the different types of samples, the techniques used in the sample collection, preparation and measurements.…”

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2021

Veterinarni Medicina

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The objective of this study was to evaluate the concentrations of the total (T-GSH), oxidised (GSSG) and reduced (GSH) glutathione in the blood of dairy cows, assess the relationships of the phase and number of lactation, milk production, body condition score (BCS) and selected biochemical parameters on its concentrations. We analysed 79 samples of whole blood from dairy Holstein cows. The concentration of glutathione was assessed by the spectrophotometric enzymatic method. The whole dataset shows the mean concentration of T-GSH, GSSG and GSH of 803 ± 22 µmol/l, 23 ± 2.5 µmol/l and 757 ± 27 µmol/l, respectively. The GSH/GSSG ratio was 95 ± 17. The phase of the lactation had a significant impact on the levels of T-GSH and GSH, but the number of lactation and BCS had no effect. Dry cows had higher levels of T-GSH (938 ± 44 µmol/l) than the fresh (713 ± 46 µmol/l) and peak lactation (785 ± 45 µmol/l) cows. The fresh cows had significantly lower concentrations of GSH (618 ± 44 µmol/l) than the peak lactation (719 ± 46 µmol/l) and dry cows (827 ± 43 µmol/l). On the basis of a regression analysis, blood glutathione was affected mainly by the liver function and energy metabolism. Glutathione as the marker of oxidation stress seems to be a promising tool in monitoring the health and welfare of the herd, yet intensive research in this field remains necessary.

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2021

Veterinarni Medicina

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“…Electrochemical separation by isotachophoresis (ITP) seems to be another suitable method. It enables specific determination of both GSH and GSSG in the same sample at the same time (Bodor et al 2018). Nevertheless, no standard method of glutathione assessment exists and even discrepancies in sample preparation and units of GSH and GSSG levels aggravate the comparison of results among individual experiments.…”

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The evaluation of glutathione concentration in whole blood of Holstein dairy calves

et al. 2019

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The aim of this study was to evaluate the concentrations of total (T-GSH), oxidized (GSSG) and reduced glutathione (GSH) in blood of clinically healthy calves, to assess the effect of sex and age together with the type of feeding on its concentrations, and to assess the relationships between glutathione and metabolic indices. A total of 117 Holstein calves at the age of 1 day to 6 months were divided according to age and together with the type of feeding into 4 groups: colostrum and transition milk (n = 20), native milk (n = 39), milk replacer (n = 29) and total mixed ration (n = 29). Blood serum was used for the assessment of the metabolic profile of calves. The concentrations of individual forms of glutathione (mean ± SEM) were as follows: T-GSH: 789.1 ± 19.4 µmol/l, GSSG: 54.2 ± 4.3 µmol/l, GSH: 681.6 ± 15.3 µmol/l, and GSH/GSSG: 41.1 ± 5.9, with the coefficients of variability of 26.6%, 85.3%, 24.3% and 157.4%, respectively. Age and the type of feeding affected GSSG (P ≤ 0.01) and GSH/GSSG (P ≤ 0.01). The relationships between glutathione and biochemical parameters were mostly weak. T-GSH correlated with creatinine (P ≤ 0.05) and chlorides (P ≤ 0.05); GSSG correlated with albumin (P ≤ 0.01), creatinine (P ≤ 0.01), cholesterol (P ≤ 0.05), aspartate aminotransferase (P ≤ 0.01), alanine aminotransferase (P ≤ 0.01), gamma-glutamyltransferase (P ≤ 0.05) and calcium (P ≤ 0.05); GSH/GSSG ratio correlated with creatinine (P ≤ 0.01), cholesterol (P ≤ 0.05), aspartate aminotransferase (P ≤ 0.05), gamma-glutamyltransferase (P ≤ 0.01) and calcium (P ≤ 0.05). The evaluation of individual forms of glutathione helps in estimation of redox status in healthy animals and their monitoring can detect the stress extent.

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Recent developments in capillary and microchip electroseparations of peptides (2017–mid 2019)

Kašička

2019

Electrophoresis

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The review presents a comprehensive survey of recent developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) for analysis, micropreparation, and physicochemical and biochemical characterization of peptides since 2017 up to about the middle of 2019. Progress in the study of electromigration properties of peptides and in the methodology of their analysis (sample preseparation, preconcentration and derivatization, adsorption suppression, EOF control, and detection) are described. Advances in CE and CEC methods are demonstrated and their applications in the following areas are presented: qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence and chiral analysis, and peptide mapping of proteins. In addition, micropreparative separations and determinations of important physicochemical characteristics of peptides by CE and CEC methods are reported.

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Capillary Isotachophoresis Determination of Trace Oxidized Glutathione in Blood

Cited by 5 publications

References 17 publications

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The evaluation of glutathione concentration in whole blood of Holstein dairy calves

Recent developments in capillary and microchip electroseparations of peptides (2017–mid 2019)

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