Capillary zone electrophoresis determination of galanthamine in biological fluids and pharmaceutical preparatives: Experimental design and artificial neural network optimization

Pokorná, Lenka; Revilla, Alma L; Havel, Josef; Patočka, Jiří

doi:10.1002/(sici)1522-2683(19990701)20:10<1993::aid-elps1993>3.0.co;2-5

Cited by 24 publications

(7 citation statements)

References 14 publications

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“…The sample was spiked with rivastigmine in order to establish the ability of the method to separate the drug from other components that might exist in the blood sample. In this study, the blood sample was not directly injected into the capillary, because some components that exist in the sample can be absorbed to the capillary wall and deteriorate the performance of the column [34]. The blood sample was therefore diluted ten folds with the BGE [12.5 mM Na2HPO4 / 12.5 mM Na2B4O7 / 20 mM SDS (pH 10)], and it was then spiked with 25 μg/mL of rivastigmine.…”

Section: Methods Developmentmentioning

confidence: 99%

Method Development by Use of Capillary Electrophoresis and Applications in Pharmaceutical, Biological and Natural Samples

Kapnissi‐Christodoulou¹

2012

Electrophoresis

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In addition, hydrogen bonding, dipole-dipole, and dispersive interactions can contribute to the solute partitioning between the two phases [4][5][6].CE was originally considered as a powerful analytical tool for the analysis of biological macromolecules. It has though, over the years, been extensively used for the separation of other compounds, such as chiral drugs, food additives, pesticides, inorganic ions, organic acids, and others. In this chapter, the ability of CE, and particularly CZE and MEKC, to be used for the qualitative and quantitative determination of compounds in pharmaceutical, biological and natural samples is investigated. In each approach, a number of studies are reported and discussed. These studies involve establishment of optimum separation conditions, method validation, optimization of sample-preparation procedure and application for the determination of the analytes under study in real samples. The first part of this chapter involves the determination of polyphenolic compounds using CZE with UVVis detector in red and white wines, while the second part involves the determination of pharmaceutical compounds in biological samples, such as blood and urine, using the hyphenated technique CE-MS (mass spectrometry). The third and final part emphasizes the importance of MEKC in chiral analysis since it has been known that usually only one enantiomer is active, while the other may be less active, inactive or has adverse effects. Determination of polyphenolic compounds in natural samplesPolyphenolic compounds exist in a variety of natural products, such as fruits, vegetables, beverages (tea, wine and juices), honey, cacao and herbs. They attract a lot of interest due to their beneficial implication in human health. They have been widely studied due to their antioxidant capacity and their association with several pathological conditions, such as hypertension, cardiovascular disease, dementia, and even cancer [7][8][9]. Therefore, due to their health significance, numerous analytical methods have, over the last decades, been developed for their separation, identification and quantitation in natural products [10][11][12][13].According to literature, the simplest CE method, CZE, proved to be the best method for the determination of polyphenolic compounds in wine samples [14][15][16][17]. In such studies, and in each case, when the optimum CZE method was applied to different red and white wines, it was established that red wines have higher levels of polyphenolic compounds than white wines and that the polyphenolic composition varies among different wines.

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Section: Methods Developmentmentioning

confidence: 99%

Method Development by Use of Capillary Electrophoresis and Applications in Pharmaceutical, Biological and Natural Samples

Kapnissi‐Christodoulou¹

2012

Electrophoresis

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“…Literature search has revealed that donepezil has been determined in human plasma by liquid chromatography/tandem mass spectrometry [4], high performance liquid chromatography with fluorescence detection [5]. Capillary zone electrophoretic technique [6] and highperformance liquid chromatography-tanden mass spectrometry (HPLC-MS/MS) [7] have been employed to determine galanthamine in biological fluids. The determination of rivastigmine, a relatively new drug used for the treatment of Alzheimer's disease has been done using flurometric method [8], HPLC [9], liquid chromatography-tandem mass spectrometry (LC-MS-MS) [10].…”

Section: Editorialmentioning

confidence: 99%

Alzheimer’s Disease: The Role of Hyphenated Analytical Systems in the Analysis of Therapeutic Agents Effective against the Disease in Biological Fluids

John¹

2018

APCT

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“…The sample was spiked with rivastigmine in order to establish the ability of the method to separate the drug compound from all the others components. If blood sample is directly injected into the capillary, some components that exist in the sample can be absorbed to the capillary wall and deteriorate the performance of the column (13). Thus, the blood sample was initially diluted ten folds with the buffer solution [12.5 mM Na 2 HPO 4 /12.5 mM Na 2 B 4 O 7 /20 mM SDS (pH 10)] (Figure 6A).…”

Section: Applicationmentioning

confidence: 99%

“…The method developed could be used to assess distribution of the parent compounds and metabolites in body tissues and fluids following real-life exposure (12). Galanthamine has also been determined by CZE in biological fluids and pharmaceutical formulations (13). It was observed that the addition of magnesium chloride has permitted a better separation of galanthamine from the other serum components.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Nine Acetylcholinesterase Inhibitors Using Micellar Electrokinetic Chromatography

Nicolaou¹,

Kapnissi‐Christodoulou²

2011

Journal of Chromatographic Science

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MEKC was used for the separation of nine acetylcholinesterase inhibitors (AChEIs). AChEIs are an important group of drug compounds that are used medicinally to treat Alzheimer's disease and Myasthenia Gravis. At the time of the experiment, this is the first time that nine AChEIs are used simultaneously in a study. Several chromatographic parameters, such as buffer concentration, pH, surfactants and their concentration, background electrolyte composition, etc., were evaluated to optimize the separation. The optimum separation of the nine AChEIs was achieved in less than 15 min by using 12.5 mM Na(2)HPO(4), 12.5 mM Na(2)B(4)O(7) and 20 mM SDS at pH 10, an applied voltage of 30 kV and a temperature of 25 °C. The reproducibility of the method was also evaluated by computing the RSDs of the migration times and the areas of the nine analyte-peaks, and the migration time and the area of the peak that corresponds to rivastigmine added in the blood sample. The RSD values of the migration times and the peak areas were less than 2% and 6%, respectively, in most cases. The limits of detection and quantification were 0.5 μg/mL and 1.7 μg/mL, respectively. The MEKC method developed was applied to a real blood sample that was obtained from a patient who was not under any of this medication. The sample was spiked with rivastigmine in order to establish the ability of the method to separate the drug from other components that might exist in the blood sample.

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Capillary zone electrophoresis determination of galanthamine in biological fluids and pharmaceutical preparatives: Experimental design and artificial neural network optimization

Cited by 24 publications

References 14 publications

Method Development by Use of Capillary Electrophoresis and Applications in Pharmaceutical, Biological and Natural Samples

Method Development by Use of Capillary Electrophoresis and Applications in Pharmaceutical, Biological and Natural Samples

Alzheimer’s Disease: The Role of Hyphenated Analytical Systems in the Analysis of Therapeutic Agents Effective against the Disease in Biological Fluids

Simultaneous Determination of Nine Acetylcholinesterase Inhibitors Using Micellar Electrokinetic Chromatography

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