Capnocytophaga canimorsus

Gaastra, Wim; Lipman, L.J.A.

doi:10.1016/j.vetmic.2009.01.040

Cited by 101 publications

(119 citation statements)

References 83 publications

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“…Since the first case of human infection in 1976,2 approximately 200 cases of C canimorsus infection have been reported worldwide 3. The time to the onset of symptoms after animal contact varies widely from one to eight days 4 .…”

Section: Discussionmentioning

confidence: 99%

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Shock following a cat scratch

2013

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A 49-year-old man with fever, pain in both legs, purpuras and cyanosis was admitted to hospital. He was a heavy drinker, but did not have diabetes or other immunosuppressive disease. On admission, he was in shock, with haematological findings suggestive of disseminated intravascular coagulation, and liver and kidney failure. The presence of a scratch wound on his face caused by a cat, and linear, Gram-negative rods phagocytosed by polynuclear leucocytes on peripheral blood smear suggested Capnocytophaga canimorsus infection. On day 1, antibiotics (ampicillin/sulbactam) and catecholamines were initiated. The patient required haemodialysis three times per week for 3 weeks. His toes became necrotic but improved and amputation was not necessary. On day 52, he was discharged from hospital with only mild sensory impairment of the legs.

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Section: Discussionmentioning

confidence: 99%

“…The slow growth of the bacterium in regular culture and residual effects of previous antibiotic treatment often make it difficult to obtain an early definitive diagnosis. Aztreonam, polymyxins and aminoglycosides are reportedly ineffective, and penicillin combined with β-lactamase inhibitors is a first-line choice 3 9…”

Section: Discussionmentioning

confidence: 99%

Shock following a cat scratch

2013

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“…To test specificity, C. canimorsus was added to the mock communities. This strain is usually found in the microbiota of canines [72]. The one marginally positive result found with ParoCheck®Kit 20 may reflect an unspecific primer attachment due to the presence of C. canimorsus in the respective samples.…”

Section: Discussionmentioning

confidence: 99%

Accuracy of commercial kits and published primer pairs for the detection of periodontopathogens

et al. 2016

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ObjectivesDespite the input of microbiome research, a group of 20 bacteria continues to be the focus of periodontal diagnostics and therapy. The aim of this study was to compare three commercial kits and laboratory-developed primer pairs for effectiveness in detecting such periodontopathogens.Materials and methodsFourteen bacterial mock communities, consisting of 16 randomly assembled bacterial strains, were used as reference standard for testing kits and primers. Extracted DNA from mock communities was analyzed by PCR in-house with specific primers and forwarded for analysis to the manufacturer’s laboratory of each of the following kits: ParoCheck®Kit 20, micro-IDent®plus11, and Carpegen® Perio Diagnostik.ResultsThe kits accurately detected Fusobacterium nucleatum, Prevotella intermedia/Prevotella nigrescens, Parvimonas micra, Aggregatibacter actinomycetemcomitans, Campylobacter rectus/showae, Streptococcus mitis, Streptococcus mutans, and Veillonella parvula. The in-house primers for F.nucleatum were highly specific to subtypes of the respective periopathogen. Other primers repeatedly detected oral pathogens not present in the mock communities, indicating reduced specificity.ConclusionsThe commercial kits used in this study are reliable tools to support periodontal diagnostics. Whereas the detection profile of the kits is fixed at a general specificity level, the design of primers can be adjusted to differentiate between highly specific strains. In-house primers are more error-prone. Bacterial mock communities can be established as a reference standard for any similar testing.Clinical relevanceThe tested kits render good results with selected bacterial species. Primers appear to be less useful for routine clinical diagnostics and of limited applicability in research. Basic information about the periodontopathogens identified in this study supports clinical decision-making.

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“…While other Capnocytophaga species have been reported to produce beta-lactamase, this has not been found in C. canimorsus [4]. Active antibiotics for C. canimorsus include penicillin, ampicillin, beta-lactam/beta-lactamase combinations, third-generation cephalosporins, imipenem, clindamycin, linezolid, tetracyclines, chloramphenicol, and fluoroquinolones [5].…”

Section: Discussionmentioning

confidence: 99%