Capoeta capoeta (Guldensteadt 1773)’larda Kurşun (II) Asetat Toksisitesine Karşı L-Karnitinin Koruyucu Etkileri

Abstract

In this study, the protective effects of L-carnitine (LC) against Lead (II) acetate (Pb(C2H3O2)2) toxicity in Capoeta capoeta were investigated by means of histopathologically, via electrophoretic and biochemical methods. Fish caught from Kars creek were divided into four groups, which include 10 fish each in 500 L tanks. Lead and liquid LC were added to water. Fish in the first group were adjusted as control. Fish in the II. group were applied 1 mg/L lead (as Lead (II) acetate) for 10 days. Fish in the III. group were administered 100 mg/L LC. Fish in the IV. group were administered 1 mg/L lead and 100 mg/L LC. Degenerations in liver, gill, intestine and kidney tissues were observed to reduce LC administration against the toxic effects of lead acetate. Electrophoretically, inhibitions of some protein bands in the group, which was applied lead acetate, were caused, and increases in protein expression in the group, which was applied L-carnitine, have occurred. While high a level of total protein in the group that was administered lead was found; in the group that was treated lead + LC, it was found to be lower (P<0.05). Levels of globulin in the group that was administered LC + lead were observed to be significantly lower (P<0.05). Total oxidant capacity (TOC) in lead treatment group were higher than the control group, TOC levels in lead + LC treatment group were determined to be between the control and lead group. LC was concluded to show a protective effect on Capoeta capoeta that were exposed to lead. Keywords: Lead (II) acetate, L-carnitine, Capoeta capoeta, Electrophoresis, TAC, TOC, Histopathology Capoeta capoeta (Guldensteadt 1773)'larda Kurşun (II) Asetat Toksisitesine Karşı L-Karnitinin Koruyucu EtkileriÖzet Çalışmada, Capoeta capoeta'da Kurşun (II) asetat (Pb(C2H3O2)2) toksisitesine karşı L-karnitinin (LK) koruyucu etkileri histopatolojik, elektroforetik ve biyokimyasal yöntemlerle incelendi. Kars Çayı'ndan yakalanan balıklar 500 L'lik tanklarda her grupta 10'ar adet balık bulunan 4 gruba ayrıldı. Kurşun ve sıvı LK suya ilave edildi. I. gruptaki balıklar kontrol grubu olarak belirlendi. II. gruptaki balıklara 10 gün süreyle 1 mg/L kurşun asetat uygulandı. III. gruptaki balıklara 100 mg/L LK uygulandı. IV. gruptaki balıklar ise 1 mg/L kurşun ve 100 mg/L dozunda LK uygulandı. Karaciğer, solungaç, bağırsak ve böbrek dokularında kurşun asetatın toksik etki gösterdiği ve buna karşı L-karnitin uygulamasının koruyucu etki göstererek oluşan bu dejenerasyonların şiddetini azalttığı gözlemlendi. Elektroforetik incelemede, kurşun asetatın birçok protein bandında inhibisyona neden olduğu, LK uygulaması sonucunda da protein ekspresyonlarında artış meydana geldiği saptandı. Kurşun uygulanan grupta total protein düzeyinin yüksek, kurşun + LK verilen grubun ise düşük olduğu belirlendi (P<0.05). Globulin düzeylerinin, LK + kurşun verilen grupta istatistiksel olarak düşük olduğu saptandı (P<0.05). Kurşun uygulanan grubun Total Oksidan Kapasitesinin (TOK) kontrol grubuna göre yüksek, kurşun + LK verilen grubun TOK...