Capping of Mn-Doped ZnS Quantum Dots with DHLA for Their Stabilization in Aqueous Media: Determination of the Nanoparticle Number Concentration and Surface Ligand Density

Garcia-Cortes, Marta; González, Emma Sotelo; Fernández-Argüelles, Marı́a Teresa; Encinar, Jorge Ruiz; Costa‐Fernández, José M.; Sanz‐Medel, Alfredo

doi:10.1021/acs.langmuir.7b00409

Cited by 32 publications

(27 citation statements)

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“…Colloidal water-soluble L-cysteine-capped Mn-ZnS QDs were synthesized following a previously reported protocol, using an Mn:Zn molar ratio of 3% [ 20 ]. A further surface QD functionalization step was required to control the bioconjugation process between QDs and antibodies.…”

Section: Methodsmentioning

confidence: 99%

Catalytic Gold Deposition for Ultrasensitive Optical Immunosensing of Prostate Specific Antigen

Cid-Barrio

Encinar

Costa‐Fernández

2020

Sensors

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A major challenge in the development of bioanalytical methods is to achieve a rapid and robust quantification of disease biomarkers present at very low concentration levels in complex biological samples. An immunoassay platform is presented herein for ultrasensitive and fast detection of the prostate-specific antigen (PSA), a well-recognized cancer biomarker. A sandwich type immunosensor has been developed employing a detection antibody labeled with inorganic nanoparticles acting as tags for further indirect quantification of the analyte. The required high sensitivity is then achieved through a controlled gold deposition on the nanoparticle surface, carried out after completing the recognition step of the immunoassay, thus effectively amplifying the size of the nanoparticles from nm to µm range. Due to such an amplification procedure, quantification of the biomolecule could be carried out directly on the immunoassay plates using confocal microscopy for measurement of the reflected light produced by gold-enlarged nanostructures. The high specificity of the immunoassay was demonstrated with the addition of a major abundant protein in serum (albumin) at much higher concentrations. An extremely low detection limit for PSA quantification (LOD of 1.1 fg·mL−1 PSA) has been achieved. Such excellent LOD is 2–3 orders of magnitude lower than the clinically relevant PSA levels present in biological samples (4–10 ng·mL−1) and even to monitor eventual recurrence after clinical treatment of a prostate tumor (0.1 ng·mL−1). In fact, the broad dynamic range obtained (4 orders of magnitude) would allow the PSA quantification of diverse samples at very different relevant levels.

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Section: Methodsmentioning

confidence: 99%

Catalytic Gold Deposition for Ultrasensitive Optical Immunosensing of Prostate Specific Antigen

Cid-Barrio

Encinar

Costa‐Fernández

2020

Sensors

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“…However, the achievable detection limits (LOD) depend on the element(s) of interest, instrument, experimental conditions, and possible spectroscopic interferences between the analyte elements. For NM analysis, ICP-MS and ICP-OES are often used in connection with an upstream particle separation method like high performance liquid chromatography (HPLC) or asymmetric flow field-flow fractionation (AF4) [165][166][167][168][169][170].…”

Section: Mass Spectrometry and Atomic Spectroscopymentioning

confidence: 99%

Analyzing the surface of functional nanomaterials—how to quantify the total and derivatizable number of functional groups and ligands

et al. 2021

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Functional nanomaterials (NM) of different size, shape, chemical composition, and surface chemistry are of increasing relevance for many key technologies of the twenty-first century. This includes polymer and silica or silica-coated nanoparticles (NP) with covalently bound surface groups, semiconductor quantum dots (QD), metal and metal oxide NP, and lanthanide-based NP with coordinatively or electrostatically bound ligands, as well as surface-coated nanostructures like micellar encapsulated NP. The surface chemistry can significantly affect the physicochemical properties of NM, their charge, their processability and performance, as well as their impact on human health and the environment. Thus, analytical methods for the characterization of NM surface chemistry regarding chemical identification, quantification, and accessibility of functional groups (FG) and surface ligands bearing such FG are of increasing importance for quality control of NM synthesis up to nanosafety. Here, we provide an overview of analytical methods for FG analysis and quantification with special emphasis on bioanalytically relevant FG broadly utilized for the covalent attachment of biomolecules like proteins, peptides, and oligonucleotides and address method- and material-related challenges and limitations. Analytical techniques reviewed include electrochemical titration methods, optical assays, nuclear magnetic resonance and vibrational spectroscopy, as well as X-ray based and thermal analysis methods, covering the last 5–10 years. Criteria for method classification and evaluation include the need for a signal-generating label, provision of either the total or derivatizable number of FG, need for expensive instrumentation, and suitability for process and production control during NM synthesis and functionalization. Graphical abstract

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“…In the last years, advances carried out in the rational design of nanomaterials have contributed to the development of hybrid NPs that combine the interesting size-and shapedependent properties of semiconductor QDs with a long-lived phosphorescence-type emission. In this sense, incorporation of suitable atoms or ions into host lattices have generated a novel type of QDs with that are very promising in bioanalytical applications [30].…”

Section: Metal-doped Qdsmentioning

confidence: 99%

Quantum Dot bioconjugates for diagnostic applications

Díaz‐González

Escosura‐Muñiz

Fernández-Argüelles

et al. 2020

Topics in Current Chemistry Collections

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Quantum Dots (QDs) are a special type of engineered nanomaterials with outstanding optoelectronic properties make them as a very promising alternative to conventional luminescent dyes for biomedical applications, including biomolecule targeting, luminescence imaging as well as in drug delivery.A key parameter to ensure successful biomedical applications of QDs is that the surface of these nanomaterials should be modified with appropriate functional groups to ensure stability in aqueous solutions and conjugated with recognition elements capable to ensure an efficient tagging of the biomolecules of interest.Here, we present a review summarizing most relevant strategies for QDs surface modification and for their conjugation to biomolecules for preparation of nanoplatforms for luminescent biomolecule sensing and imaging-guided targeting. Applications of conjugations of photoluminescent QDs with different biomolecules in both in vitro and in vivo chemical sensing, immunoassays or luminescence imaging are revised. Recent progress in application of functionalized QDs in ultrasensitive detection in bioanalysis, diagnostics and imaging strategies developments are here included. Finally, some key future research goals in the progress of bioconjugation of QDs for diagnosis are identified, including novel synthetic approaches, the need of exhaustive characterization of bioconjugates or the design of signal amplification schemes.

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Capping of Mn-Doped ZnS Quantum Dots with DHLA for Their Stabilization in Aqueous Media: Determination of the Nanoparticle Number Concentration and Surface Ligand Density

Cited by 32 publications

References 50 publications

Catalytic Gold Deposition for Ultrasensitive Optical Immunosensing of Prostate Specific Antigen

Catalytic Gold Deposition for Ultrasensitive Optical Immunosensing of Prostate Specific Antigen

Analyzing the surface of functional nanomaterials—how to quantify the total and derivatizable number of functional groups and ligands

Quantum Dot bioconjugates for diagnostic applications

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