Caprine demineralized bone matrix (DBMc) in the repair of non-critical bone defects in rabbit tibias. A new bone xenograft

Abstract

To evaluate the use of demineralized bone matrix of caprine origin in experimental bone defects of the tibia in New Zealand rabbits. Methods: Fragments of the tibia diaphysis were collected aseptically from clinically healthy goats. The bones were sectioned into 1 cm fragments and stored at-20°C for subsequent hydrochloric acid (HCL) demineralization. A 70 mg portion of DBMc was used to fill the experimental bone defects. Twenty-four female adult New Zealand rabbits were divided into 2 groups: the MG (matrix group, left tibia) and CG (control group, right tibia). Additionally, they were separated into 4 groups with 6 animals, according to the period of analysis (15, 30, 60 and 90 days postoperatively). Using microCT, volumetric parameters were evaluated: bone volume, relationship between bone volume and total volume, bone surface area, relationship between bone surface area and total volume, number of trabeculae, trabecular thickness and trabecular separation. Results: There was a statistically significant difference (P<0.05) between groups considering bone volume (BV) and bone:total volume (BV/TV), on 15, 30 and 90 days postoperatively. Control group showed a statistically significant superiority (P < 0.05) considering the mean of the variables bone surface (BS), number of trabeculae (Tb.N) and between bone surface and total volume (BS/TV) at 15 and 90 days. Conclusions: Caprine demineralized bone matrix was safe and tolerable. No signs of material rejection were seen macroscopically. It is an alternative for the treatment of bone defects when autologous graft is not available or in insufficient quantities.

Research Hotspots and Trends of Bone Xenograft in Clinical Procedures: A Bibliometric and Visual Analysis of the Past Decade

“…To determine the effectiveness of bone-augmentation procedures and ensure implant stability, numerous researchers have studied the impact of various bone-augmentation techniques. CBCT enables researchers to quantitatively and intuitively measure and track changes in the contour of the alveolar ridge following tooth extraction and changes in bone elevation after MSFE [ 24 , 35 ], confirming the effectiveness of bone xenograft in various bone-augmentation techniques [ 36 , 37 , 38 , 39 , 40 , 41 , 42 ].…”

Histopathological and serological assessment of using rib lamb xenograft reinforced with and without hydroxyapatite nano gel for reconstruction tibial bone defect in dogs

“…Demineralization prevents rejection of implant as it has osteoinductive characteristics, but has no osteoconductive properties, which is produced by a careful selecting mineral from the bone without destroying the growth factors and other bone morphogenetic proteins (9). The demineralized bone matrix was used as a bone xenograft in the repair of non-critical bone defects in rabbit tibias (10) and xenogeneic bone putty is used with the carrier of hydrogel derived from the demineralized bone matrix (11). Hydroxyapatite (HAp) was widely used as a bone implant for more than four decades in orthopedics and this may be due to their similarity to a bone structural matrix including mineral composition it is such as biocompatibility, bioactivematerial, osteoconduction, slow-degradation, osteoinduction, and osteointegration (12).…”