Caprine herpesvirus-1 (CapHV-1) induces apoptosis in goat peripheral blood mononuclear cells

Pagnini, Ugo; Montagnaro, Serena; Monteforte, Edoardo Sanfelice Di; Pacelli, F.; Martino, Luisa De; Roperto, Sante; Florio, Salvatore; Iovane, G.

doi:10.1016/j.vetimm.2004.10.003

Cited by 14 publications

(18 citation statements)

References 41 publications

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“…Cell extracts, obtained by three cycles of freezing and thawing, were pooled, collected, and stored in aliquots at À808C until used. In some experiments FeHV-1 was inactivated by a photochemical treatment [Pagnini et al, 2005] that includes an incubation with a psoralen compound (trioxsalen) and an exposure to UV light. The trioxsalen (4,5,8-trimethylpsoralen) (Sigma, Milan, Italy) was dissolved in dimethyl sulfoxide (DMSO) at a concentration of 200 mg/ml.…”

Section: Virusmentioning

confidence: 99%

Feline herpesvirus‐1 down‐regulates MHC class I expression in an homologous cell system

Montagnaro

Longo

Pacilio

et al. 2008

J of Cellular Biochemistry

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Cytotoxic T lymphocytes (CTLs) are an essential component of the immune defense against many virus infections. CTLs recognize viral peptides in the context of the major histocompatibility complex (MHC) class I molecules on the surface of infected cells. Many viruses have evolved mechanisms to interfere with MHC class I expression as a means of evading the host immune response. In the present research we have studied the effect of in vitro Feline Herpesvirus 1 (FeHV-1) infection on MHC class I expression. The results of this study demonstrate that FeHV-1 down regulates surface expression of MHC class I molecules on infected cells, presumably to evade cytotoxic T-cell recognition and, perhaps, attenuate induction of immunity. Sensitivity to UV irradiation and insensitivity to a viral DNA synthesis inhibitor, like phosphonacetic acid, revealed that immediate early or early viral gene(s) are responsible. Use of the protein translation inhibitor cycloheximide confirmed that an early gene is primarily responsible.

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Section: Virusmentioning

confidence: 99%

Feline herpesvirus‐1 down‐regulates MHC class I expression in an homologous cell system

Montagnaro

Longo

Pacilio

et al. 2008

J of Cellular Biochemistry

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“…In addition, certain viruses induce apoptosis at late stages of replication, providing a mechanism for dissemination of progeny virus. Apoptotic changes in Herpesvirus-infected cells had been documented for several herpesviruses [Delhon et al, 2002;Pagnini et al, 2005;Gardell et al, 2006;Odeberg et al, 2006;Longo et al, 2009;Brazeau et al, 2010], in the present study we described the mechanism of cell death in a neuroblastoma cell line in order to investigate on the apoptotic process induced by CpHV-1 during infection.…”

Section: Discussionmentioning

confidence: 89%

“…Among stimuli that have been associated with apoptosis are infections with mammalian DNA and RNA viruses [Hay and Kannourakis, 2002]. Viruses possess various biochemical and genetic mechanisms to evade and/or induce apoptosis in infected cells through interactions at different stages of the apoptotic pathway and it has been demonstrated that apoptosis modulation through virusencoded proteins is a key step in herpesviruses pathogenesis [Henderson et al, 1993;Hu et al, 1997;Wang et al, 1997;Cassady et al, 1998;Pagnini et al, 2004;Pagnini et al, 2005;Marfè et al, 2006;Longo et al, 2009].…”

mentioning

confidence: 99%

“…Apoptotic phenomenon has been observed after infections with herpes simplex virus (HSV) type 2 (in the brain) [Geiger et al, 1995], varicella-zoster virus (in cultured cells) [Sadzot-Delvaux et al, 1995;Brazeau et al, 2010], and CpHV-1 (in blood mononuclear cells) [Pagnini et al, 2005]. Moreover, several herpesvirses such as HSV, VZV, and EHV-1 show a marcked neurotropism and neurovirulence [Watanabe et al, 2000;Pugazhenthi et al, 2011;Mori et al, 2012].…”

mentioning

confidence: 99%

“…Infection in adult goats remains unapparent, or may cause respiratory distress, abortion [Berrios et al, 1975;Waldvogel et al, 1981;Williams et al, 1997], vulvovaginitis or balanoposthitis [Horner et al, 1982;Tisdall et al, 1984]. Moreover, being a member of alphaherpesvirus, CpHV-1 is able to establish latent infection in trigeminal ganglia [Plebani et al, 1983;Buonavoglia et al, 1996] and to cause immunosuppression [Pagnini et al, 2005]. Thus, CpHV-1 infection is responsible for severe economic losses due to abortions, stillbirths, reproductive disorders, and susceptibility of CpHV-1-infected host to secondary bacterial infections.…”

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confidence: 99%

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Modulation of apoptosis by caprine herpesvirus 1 infection in a neuronal cell line

Montagnaro

Ciarcia

Martinis

et al. 2013

J of Cellular Biochemistry

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Caprine herpesvirus type 1 (CpHV-1), like other members of the alpha subfamily of herpesviruses, establishes latent infections in trigeminal ganglion neurons. Our groups previously demonstrated that CpHV-1 induces apoptosis in goat peripheral blood mononuclear cells and in an epithelial bovine cell line, but the ability of CpHV-1 to induce apoptosis in neuronal cells remains unexplored. In this report, the susceptibility of Neuro 2A cells to infection by CpHV-1 was examined. Following infection of cultured cells with CpHV-1, expression of cell death genes was evaluated using real-time PCR and Western blot assays. Analysis of virus-infected cells revealed activation of caspase-8, a marker for the extrinsic pathway of apoptosis, and caspase-9, a marker for the intrinsic pathway of apoptosis at 12 and 24 h post-infection. Significant increase in the levels of cleaved caspase-3 was also observed at the acme of cytopathic effect at 24 h post-infection. In particular, at 3 and 6 h post-infection, several proapototic genes were under-expressed. At 12 h post-infection several proapototic genes such as caspases, TNF, Cd70, and Traf1 were over expressed while Bcl2a1a, Fadd, and TNF genes were underexpressed. In conclusion, the simultaneous activation of caspase-8 and caspase-9 suggests that CpHV-1 can trigger the death-receptor pathway and the mitochondrial pathway separately and in parallel. Our findings are significant because this is the first published study showing the effect of CpHV-1 infection in neuronal cells in terms of gene expression and apoptosis modulation.

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Microvascular lesions and changes in cell proliferation and death, and cytokine expression in the placentas of mice experimentally infected with Equid Herpesvirus 1

Zanuzzi

Bravi

Scrochi

et al. 2016

Research in Veterinary Science

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Caprine herpesvirus-1 (CapHV-1) induces apoptosis in goat peripheral blood mononuclear cells

Cited by 14 publications

References 41 publications

Feline herpesvirus‐1 down‐regulates MHC class I expression in an homologous cell system

Feline herpesvirus‐1 down‐regulates MHC class I expression in an homologous cell system

Modulation of apoptosis by caprine herpesvirus 1 infection in a neuronal cell line

Microvascular lesions and changes in cell proliferation and death, and cytokine expression in the placentas of mice experimentally infected with Equid Herpesvirus 1

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