Caprine uterine and placental osteopontin expression is distinct among epitheliochorial implanting species

Joyce, Margaret M.; Gonzalez, Julissa; Lewis, Shaye; Woldesenbet, Selamawit; Burghardt, Robert C.; Newton, G. R.; Johnson, Greg A.

doi:10.1016/j.placenta.2004.05.009

Cited by 39 publications

(26 citation statements)

References 67 publications

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“…Regarding implantation, studies have focused on the ability of OPN to support integrin-mediated cell adhesion and cell migration (Senger et al 1994 ). It is likely that the expression of no other ECM protein is conserved across different species to the degree that has been observed for OPN, which is expressed abundantly within the conceptus-uterine environment of humans, mice, rabbits, sheep, goats, and pigs (Johnson et al 1999a , b ;Garlow et al 2002 ;Apparao et al 2003 ;Mirkin et al 2005 ;Joyce et al 2005 ;White et al 2006 ). In pigs, expression of OPN is initially induced by conceptus estrogens in discrete regions of the uterine LE juxtaposed to the conceptus trophectoderm just prior to implantation on day 13, expands to the entire uterine LE by day 20 when fi rm adhesion of conceptus trophectoderm to uterine LE occurs, and remains high at this interface throughout pregnancy White et al 2005 ).…”

Section: Trophoblast Attachment and Adhesionmentioning

confidence: 99%

Implantation and Establishment of Pregnancy in the Pig

Geisert¹,

Johnson²,

Burghardt

2015

Regulation of Implantation and Establishment of Pregnancy in Mammals

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Establishment of pregnancy in the pig is initiated through the release of estrogens from the rapidly elongating conceptuses. Release of estrogens from the developing conceptuses alters the movement of endometrial prostaglandin F2α from being released into the vasculature (endocrine secretion) to sequestering in the uterine lumen (exocrine secretion). Rapid trophoblast elongation, which is unique to the pig, may be triggered through production of interleukin 1β2 (IL1B2) by conceptuses. Trophoblast elongation through the uterine horns provides the mechanism to allow conceptus-endometrial interactions essential for the implantation, placentation, and maintenance of pregnancy in the pig. This chapter provides current information on conceptus signaling pathways and endometrial responses to those conceptus factors leading to establishment of pregnancy.

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Section: Trophoblast Attachment and Adhesionmentioning

confidence: 99%

Implantation and Establishment of Pregnancy in the Pig

Geisert¹,

Johnson²,

Burghardt

2015

Regulation of Implantation and Establishment of Pregnancy in Mammals

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“…We previously reported that a 45 kDa form of SPP1, with increased cell adhesion properties compared with full-length SPP1 (Agnihotri et al 2001), is secreted by uterine glands under the influence of progesterone into the uterine lumen in sheep during pregnancy (Johnson et al 2000), and is increasingly deposited at the maternal-conceptus interface throughout pregnancy in sheep (Johnson et al 2001) and other mammals (Johnson et al 1999a, 1999b, Apparao et al 2001, Joyce et al 2005, White et al 2006. SPP1 is a monomer of 278 amino acids, which undergoes extensive posttranslational modification including phosphorylation, glycosylation, and cleavage by thrombin, MMP3, and MMP7, resulting in molecular mass variants ranging from 24 to 75 kDa (Johnson et al 1999a, 1999b, Agnihotri et al 2001, and a predicted secondary structure has eight a-helices and six b-sheet segments.…”

Section: Spp1 and Lgals15 Are Potential Ecm Mechanosensors At The Matmentioning

confidence: 99%

Enhanced focal adhesion assembly reflects increased mechanosensation and mechanotransduction at maternal–conceptus interface and uterine wall during ovine pregnancy

Burghardt¹,

Burghardt²,

Taylor³

et al. 2009

Reproduction

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The integrity of the fetal-maternal interface is critical for proper fetal nourishment during pregnancy. Integrins are important adhesion molecules present at the interface during implantation; however, in vivo evidence for integrin activation and focal adhesion formation at the maternal-conceptus interface is limited. We hypothesized that focal adhesion assembly in uterine luminal epithelium (LE) and conceptus trophectoderm (Tr) results from integrin binding of extracellular matrix (ECM) at this interface to provide increased tensile forces and signaling to coordinate utero-placental development. An ovine model of unilateral pregnancy was used to evaluate mechanotransduction events leading to focal adhesion assembly at the maternal-conceptus interface and within the uterine wall. Animals were hysterectomized on days 40, 80, or 120 of pregnancy, and uteri immunostained for integrins (ITGAV, ITGA4, ITGA5, ITGB1, ITGB3, and ITGB5), ECM proteins (SPP1, LGALS15, fibronectin (FN), and vitronectin (VTN)), cytoskeletal molecules (ACTN and TLN1), and a signal generator (PTK2). Focal adhesion assembly in myometrium and stroma was also studied to provide a frame of reference for mechanical stretch of the uterine wall. Large focal adhesions containing aggregates of ITGAV, ITGA4, ITGA5, ITGB1, ITGB5, ACTN, and PTK2 were detected in interplacentomal uterine LE and Tr of gravid but not non-gravid uterine horns and increased during pregnancy. SPP1 and LGALS15, but not FN or VTN, were present along LE and Tr interfaces in both uterine horns. These data support the idea that focal adhesion assembly at the maternal-conceptus interface reflects adaptation to increasing forces caused by the growing fetus. Cooperative binding of multiple integrins to SPP1 deposited at the maternal-conceptus interface forms an adhesive mosaic to maintain a tight connection between uterine and placental surfaces along regions of epitheliochorial placentation in sheep.

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“…In goats, integrin subunits av, a4, a5, b1, and b3 are expressed on conceptus trophectoderm and endometrial LE on Days 21 and 23 of pregnancy [64]. Thus, conceptus implantation in sheep and goats does not appear to involve changes in temporal or spatial patterns of integrin expression [63,64], but appears to depend primarily on changes in expression of integrin ligands, such as LGALS15 and secreted phosphoprotein one (SPP1/osteopontin) [5,65,66]. Other galectins bind integrins, FN, and laminin, because these extracellular matrix proteins are modified with beta-galactoside sugars [22,23].…”

Section: Lewis Et Almentioning

confidence: 99%

Galectin 15 (LGALS15): A Gene Uniquely Expressed in the Uteri of Sheep and Goats that Functions in Trophoblast Attachment1

Lewis

Farmer

Burghardt

et al. 2007

Biology of Reproduction

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Galectins are a family of secreted animal lectins with biological roles in cell adhesion and migration. In sheep, galectin 15 (LGALS15) is expressed specifically in the endometrial luminal (LE) and superficial glandular (sGE) epithelia of the uterus in concert with blastocyst elongation during the peri-implantation period. The present study examined LGALS15 expression in the uterus of cattle, goats, and pigs. Although the bovine genome contains an LGALS15-like gene, expressed sequence tags encoding LGALS15 mRNA were found only for sheep, and fulllength LGALS15 cDNAs were cloned only from endometrial total RNA isolated from pregnant sheep and goats, but not pregnant cattle or pigs. Ovine and caprine LGALS15 were highly homologous at the mRNA (95%) and protein (91%) levels, and all contained a conserved carbohydrate recognition domain and RGD recognition sequence for integrin binding. Endometrial LGALS15 mRNA levels increased after Day 11 of both the estrous cycle and pregnancy, and were considerably increased after Day 15 of pregnancy in goats. In situ hybridization detected abundant LGALS15 mRNA in endometrial LE and sGE of early pregnant goats, but not in cattle or pigs. Immunoreactive LGALS15 protein was present in endometrial epithelia and conceptus trophectoderm of goat uteri and detected within intracellular crystal structures in trophectoderm and LE. Recombinant ovine and caprine LGALS15 proteins elicited a dose-dependent increase in ovine trophectoderm cell attachment in vitro that was comparable to bovine fibronectin. These results support the hypothesis that LGALS15 is uniquely expressed in Caprinae endometria and functions as an attachment factor important for peri-implantation blastocyst elongation.

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Caprine uterine and placental osteopontin expression is distinct among epitheliochorial implanting species

Cited by 39 publications

References 67 publications

Implantation and Establishment of Pregnancy in the Pig

Implantation and Establishment of Pregnancy in the Pig

Enhanced focal adhesion assembly reflects increased mechanosensation and mechanotransduction at maternal–conceptus interface and uterine wall during ovine pregnancy

Galectin 15 (LGALS15): A Gene Uniquely Expressed in the Uteri of Sheep and Goats that Functions in Trophoblast Attachment1

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