Capsaicin sensitive neurons role in the inflamed TMJ acute nociceptive response of female and male rats

Bellinger, Larry L.; Spears, Robert; King, Christopher; Dahm, Fred; Hutchins, Bob; Kerins, Carolyn A.; Kramer, Phillip R.

doi:10.1016/j.physbeh.2007.01.002

Cited by 25 publications

(35 citation statements)

References 56 publications

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“…Meal duration measurements were confirmed by the mechanical sensitivity testing assay, in that, a high concentration of 17β-estradiol treatment significantly attenuated hypersensitivity in the ligated rats. While both the meal duration assay and filament assay measure orofacial nociception and hypersensitivity, respectively (Guo et al, 2010; Bellinger et al, 2007) there are some differences. Mechanical sensitivity testing produces a mechanical pressure induced response, whereas meal duration is a functional measure of nociception.…”

Section: Discussionmentioning

confidence: 99%

“…Meal patterns (i.e., food intake, meal size, meal number and meal duration) were calculated using Med Assoc. Inc. and proprietary software (Bellinger et al, 2007; Guan et al, 2005; Kerins et al, 2005; Kramer and Bellinger, 2009; Kramer et al, 2010). Meal duration has been shown to be a continuous, non-invasive measure of orofacial nociception (surface and deep) in undisturbed male and female rats (Kerins et al, 2005; Kerins et al, 2003).…”

Section: Methodsmentioning

confidence: 99%

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Infusion of Gabrα6 siRNA into the trigeminal ganglia increased the myogenic orofacial nociceptive response of ovariectomized rats treated with 17β-estradiol

Kramer

Bellinger

2014

Neuroscience

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High levels of 17β-estradiol (E2) have been found to reduce inflammatory temporomandibular joint (TMJ) pain. A search for genes effected by a high concentration of estradiol showed an increase in GABAA receptor subunit α6 (Gabrα6) in the trigeminal ganglia (TG). Blockade of Gabrα6 expression in the TG increases masseter muscle nociception in male rats, but the relationship between estradiol’s effect on nociception and Gabrα6 expression remains unclear in females. To address this knowledge gap we hypothesized that reducing Gabrα6 expression in the TG will increase the orofacial nociceptive response of ovariectomized female rats treated with estradiol. To administer hormone osmotic pumps were placed in rats that dispensed a low diestrus plasma concentration of 17β-estradiol, in addition, 17β-estradiol was injected to produce a high proestrus plasma concentration of estradiol. A ligature was then placed around the masseter tendon to induce a nociceptive response; a model for TMJ muscle pain. Gabrα6 siRNA was later infused into the TG and the nociceptive response was measured using von Frey filaments and a meal duration assay. GABAA receptor expression was measured in the TG and trigeminal nucleus caudalis and upper cervical region (Vc-C1). Ligature significantly increased the nociceptive response but a high proestrus concentration of 17β-estradiol attenuated this response. Gabrα6 siRNA infusion decreased Gabrα6 expression in the TG and Vc-C1 but increased the nociceptive response after 17β-estradiol treatment. The results suggest estradiol decreased the orofacial nociceptive response, in part, by causing an increase in Gabrα6 expression.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Infusion of Gabrα6 siRNA into the trigeminal ganglia increased the myogenic orofacial nociceptive response of ovariectomized rats treated with 17β-estradiol

Kramer

Bellinger

2014

Neuroscience

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“…However, there are potential limitations of this technique including the potential neurochemical and anatomical reorganization of the remaining A-delta nerve fibers in the dorsal horn of the spinal cord and increased tissue concentrations of histamine and 5-hydroxytryptamine in the dorsal skin of the hindpaw (Holzer et al, 1981, Nagy and Hunt, 1983, Rethelyi et al, 1986, Shortland et al, 1990). While the functional significance of these alterations in pain transmission remains unclear, neonatal capsaicin treatment remains a valuable tool to evaluate the role of capsaicin-sensitive sensory nerve fibers in the development and maintenance of pain in a variety of preclinical models of acute and chronic pain (Khan et al, 2004, Bellinger et al, 2007, Nakagawa et al, 2007). …”

Section: Discussionmentioning

confidence: 99%

Capsaicin-sensitive sensory nerve fibers contribute to the generation and maintenance of skeletal fracture pain

et al. 2009

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Although skeletal pain can have a marked impact on a patient's functional status and quality of life, relatively little is known about the specific populations of peripheral nerve fibers that drive nonmalignant bone pain. In the present report, neonatal male Sprague Dawley rats were treated with capsaicin or vehicle and femoral fracture was produced when the animals were young adults (15-16 weeks old). Capsaicin treatment, but not vehicle, resulted in a significant (>70%) depletion in the density of calcitonin-gene related peptide positive (CGRP + ) sensory nerve fibers, but not 200 kD neurofilament H positive (NF200 + ) sensory nerve fibers in the periosteum. The periosteum is a thin, cellular and fibrous tissue that tightly adheres to the outer surface of all but the articulated surface of bone and appears to play a pivotal role in driving fracture pain. In animals treated with capsaicin, but not vehicle, there was a 50% reduction in the severity, but no change in the time course, of fracture-induced skeletal pain related behaviors as measured by spontaneous flinching, guarding and weight bearing. These results suggest that both capsaicin-sensitive (primarily CGRP + C-fibers) and capsaicin-insensitive (primarily NF200 + A-delta fibers) sensory nerve fibers participate in driving skeletal fracture pain. Skeletal pain can be a significant impediment to functional recovery following trauma-induced fracture, osteoporosis-induced fracture and orthopedic surgery procedures such as knee and hip replacement. Understanding the specific populations of sensory nerve fibers that need to be targeted to inhibit the generation and maintenance of skeletal pain may allow the development of more specific mechanism-based therapies that can effectively attenuate acute and chronic skeletal pain.

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“…This model uses unrestrained rats that are continuously kept in sound-attenuated, computerized feeding modules for days to weeks to record meal patterns (Kerins et al, 2005;Kramer et al, 2010). We have shown, in male and female rats and male mice, that meal duration can be used as a non-invasive biological marker for TMJ nociception for up to 19 days in rats and 6 days in mice (Kerins et al, 2003b(Kerins et al, , 2004Bellinger et al, 2007;Kramer et al, 2010). Acute or more persistent TMJ arthritis was shown to affect the rat/mouse, such that when the animal initiated a meal, the animal ate more slowly, which significantly lengthened the A non-invasive Model for Measuring nociception after tooth Pulp Exposure meal duration.…”

mentioning

confidence: 99%

“…Acute or more persistent TMJ arthritis was shown to affect the rat/mouse, such that when the animal initiated a meal, the animal ate more slowly, which significantly lengthened the A non-invasive Model for Measuring nociception after tooth Pulp Exposure meal duration. When the TMJ arthritis was reduced by pharmacological intervention, meal duration returned to normal (Kerins et al, 2003b(Kerins et al, , 2004Bellinger et al, 2007). Patients experiencing TMJ pain also have longer chewing cycles, and the cycle length is attenuated when TMJ pain is diminished (Hansdottir and Bakke, 2004;Bakke and Hansdottir, 2008;Pereira et al, 2009).…”

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confidence: 99%

A Non-invasive Model for Measuring Nociception after Tooth Pulp Exposure

Kramer

Puri

et al. 2012

J Dent Res

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Temporomandibular arthritis will lengthen a rodent's meal duration. We hypothesized that meal duration would also lengthen after tooth pulp exposure, suggesting that this behavior could be used to measure tooth nociception. To test this hypothesis, we placed rats in feeding units and subjected 4 anterior mandibular molars to pulp exposure, with and without pre-treatment with the analgesic buprenorphine-HCl. In the first study, male Sprague-Dawley rats were placed in computerized sound-attenuated feeding modules, the pulp of 4 molars on the mandible were exposed, and meal duration was measured for 13 days. In a second study, rats were injected with either the analgesic buprenorphine-HCl or saline every 12 hrs; injections were started one day before pulp exposure. Meal duration was determined before and after treatment. In the first study, pulp exposure significantly increased daily meal duration for 8 days. In the second study, pulp exposure lengthened daily meal duration, but the group that was treated with buprenorphine-HCl showed no significant difference compared with control rats without pulp exposure. Evidence supports that a lengthening in meal duration is a response to tooth nociception and that this nociception can be measured for over a week.

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Capsaicin sensitive neurons role in the inflamed TMJ acute nociceptive response of female and male rats

Cited by 25 publications

References 56 publications

Infusion of Gabrα6 siRNA into the trigeminal ganglia increased the myogenic orofacial nociceptive response of ovariectomized rats treated with 17β-estradiol

Infusion of Gabrα6 siRNA into the trigeminal ganglia increased the myogenic orofacial nociceptive response of ovariectomized rats treated with 17β-estradiol

Capsaicin-sensitive sensory nerve fibers contribute to the generation and maintenance of skeletal fracture pain

A Non-invasive Model for Measuring Nociception after Tooth Pulp Exposure

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